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R F Digiacomo

Publications and source records attributed to R F Digiacomo.

8 recordsLinked to original sources
PubMed1991

Naturally acquired Pasteurella multocida subsp. multocida infection in a closed colony of rabbits: characteristics of isolates.

Twelve litters, comprising 41 rabbits aged 35 to 60 days old, in a closed university colony, were monitored for acquisition of nasal Pasteurella multocida subsp. multocida infection. Isolates from 11 infected rabbits were characterized by colonial morphology, capsular type, biotype and antibiotic resistance. Selected isolates were further characterized by somatic antigen typing. Two major strains of P. multocida subsp. multocida were detected in the colony. One strain had mucoid colonies, fermented few carbohydrates and was serotype A:5, whereas, the other strain had smooth iridescent colonies, non-typeable capsular antigen, type 3 somatic antigen and fermented more than twice as many carbohydrates.

Animals↗
PubMed1978

Genital inoculation of male Macaca fascicularis with Neisseria gonorrhoeae and Ureaplasma urealyticum.

Inoculation of the urethra, conjunctiva, pharynx, and anal canal of six male crab-eating macaques (Macaca fascicularis) with urethral exudate from male patients with urethral gonorrhoea or with laboratory gonococcal strains was unsuccessful in establishing infection or producing increased polymorphonuclear leucocytes on Gram stain. Intraurethral inoculation with laboratory strains of Ureaplasma urealyticum resulted in transiently positive urethral cultures for U. urealyticum and was associated in some cases with increased numbers of polymorphonuclear leucocytes on Gram stain of urethral material. The findings suggest that the crab-eating macaque may be useful for studying the pathogenesis of urethral infection with U. urealyticum.

Animals↗
PubMed1975

Chlamydial infection of the male baboon urethra.

Two adult male baboons (Papio cynocephalus) were infected by urethral catheter with a Type D strain of Chlamydia trachomatis isolated from a male patient with nongonococcal urethritis. Chlamydial organisms were shed from the urethra for about 90 days and serum antibody developed. Intraurethral re-inoculation of homologous and heterologous (Type I) strains of Chalmydia, 4 and 11 months later, resulted in relatively shorter periods of infection of less than 15 days. The antibody titres and type-specific patterns were not substantially influenced by re-infection.

Animals↗
PubMedDate not supplied

Dientamoeba fragilis detection methods and prevalence: a survey of state public health laboratories.

Dientamoeba fragilis is a pathogenic protozoan parasite that has no cyst stage. Because of the lack of a cyst stage, the laboratory detection of D. fragilis in stool specimens is dependent on the stool processing and examination methods employed. Failure to use recommended stool fixation and permanent staining techniques almost precludes identification of D. fragilis, which is associated with gastrointestinal illness in humans. In this survey, questionnaires were mailed to all State and territorial public health laboratories requesting information on the number of ova and parasite examinations, methods of processing and examining stools, and the number of D. fragilis positive stools for 1985. Forty-three of 54 (80 percent) laboratories responded. Results showed that those laboratories which reported D. fragilis detection examined more stools using recommended stool fixation methods and were more likely to stain permanently all stools examined. Permanent staining of all stools, as compared to loose and watery stools only, resulted in a fivefold greater detection of D. fragilis. More State and territorial public health laboratories reported finding D. fragilis infections in 1985 than in a 1978 survey performed by the Centers for Disease Control. However, in 1985 only six laboratories reported 82 percent of all D. fragilis detections. To increase the probability of detecting D. fragilis in stool specimens, the findings suggest that all stools should be submitted fixed in polyvinyl alcohol fixative, sodium acetate-acetic acid-formalin fixative, or Schaudinn's fixative. Further, all specimens, regardless of consistency, should be permanently stained prior to microscopic examination.

Animals↗