Biomedical subjects

R F García Flores

Publications and source records attributed to R F García Flores.

At least 19 recordsLinked to original sources

PubMed1999

[Inducing high-specificity anti-cortisol antibodies for use in RIA (radioimmunoassay)].

A method for inducing highly specific cortisol antibodies for measuring plasmatic cortisol by RIA is described. The method is fast, direct (without extraction or separation), with high specificity, due to the high quality of the antibody produced in our laboratory. Reliability was demonstrated by: the sensitivity of the method, with a pattern curve of 50 pg of cortisol; the precision tests showed a variation rate intranalysis of < 9.7% and internalysis of < 17.6%; accuracy was > 87%; and specificity was demonstrated by antiserum characterization with other steroids. Measurings of cortisol in plasma of normal subjects could detect the circadian cycle pattern as well as normal supression with dexametasone. These findings support that our method allows fast measurement of plasmatic cortisol with accuracy and sensitivity, reducing operative cost.

Animals↗

PubMed1995

[Male sterility and its association with genital disease and environmental factors].

Semen quality may be affected by many factors, as there is evidence that conditions as varicocele, criptorquidia, orchitis and bacterian infections; as well as to exposure to physical agents as heat, or chemical substances, or ingestion of alcohol and drugs, may affect semen quality. The objective of this study is to investigate the risk implied in the exposure to these factors on the semen quality. The study was carried out in a prospective way in a group of males at Clínica de Infertilidad, Unidad de Biología de la Reproducción del Hospital Universitario Dr. J.E. González. Ninety nine males were studied, they received an intentioned questionnaire about antecedents of exposure to environmental factors, and urologic resolved pathology. Espermatobioscopy was done and it was classified according to OMS. Two groups were formed, one with the individuals with normal espermatobioscopy (n = 25); and the abnormal ones (n = 74). The statistical Incidences Reason, square Xi and Atributable Risk, were applied in order to determine the impact that different factors may have on semen quality. The found alterations in semen were astenozoospermia (n = 58); hypospermia (n = 22); oligozoospermia (n = 18); teratozoospermia (n = 7); polizoospermia (n = 7); and azoospermia (n = 6). The results of the mentioned statistical tests, show that in these alterations there is an associated risk factor to the use of tobacco, exposure to chemical substances, to physical aggresors; and anatomic anomalies previously corrected. It is considered that obtention of this information is a great help because once the unfavorable factors are eliminated, the environment is improved in order to obtain an espermatogenesis in optimal conditions.

Adult↗

PubMed1994

[A simplified method in determination of blood 17-hydroxyprogesterone by radioimmunoanalysis].

A practical method to measure 17-OHP, in peripheral blood by radioimmunoanalysis using a highly specific antiserum, is described. The method usefulness described in dependability studies present a sensitivity of the pattern curve of 10 picograms; precision shows a variation rate intranalysis < or = 11.9% and interanalysis of < or = 10.0%. Accuracy was > or = 95% and specificity is demonstrated by antiserum characterization with other steroids. Measuring 17-OHP4 in ng/ml in plasma of women under different physiological conditions, it was found that the levels of this hormone on day -5 of proliferative phase were 0.31 +/- 0.24 nanograms (ng)/ml, and at day +7 of secretory phase of 1.47 +/- 0.65 ng/ml. In patients with stimulation with ACTH show as to the basal sample an increase (double) of levels at 30 minutes, that keeps increasing until 90 minutes with certain trend to diminish at 120 minutes. This study shows that it is possible, to measure 17-OHP4 in human plasma with good dependability degree and easy handling reducing, in addition, operative cost.

17-alpha-Hydroxyprogesterone↗

PubMed1987