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R Fabre

Publications and source records attributed to R Fabre.

4 recordsLinked to original sources
PubMed1992

Detection by DGGE of a new polymorphism closely linked to the adenomatous polyposis coli region.

The EF5.44 locus is in close proximity to the chromosome 5 region to which the genetic defect responsible for familial adenomatous polyposis has been mapped. We have devised two oligonucleotides that promote the specific polymerase chain reaction (PCR) amplification of a 365-bp sequence in this region. Analysis by denaturing gradient gel electrophoresis of the resulting fragment has unravelled individual differences that could be identified as a single base pair change in a MnlI restriction site. This PCR assayable polymorphism increases the informativeness at this locus, and should be useful in the presymptomatic diagnosis of familial adenomatous polyposis.

Adenomatous Polyposis Coli↗
PubMed1992

Evaluation of an immunoenzymatic assay detecting specific anti-Toxocara immunoglobulin E for diagnosis and posttreatment follow-up of human toxocariasis.

In order to complete the immunodiagnosis of human toxocaral disease, an immunoenzymatic assay with excretory-secretory antigens from Toxocara canis larvae was developed for the detection of specific immunoglobulin E (sIgE enzyme-linked immunosorbent assay [ELISA]). The specificity of the assay was evaluated in patients presenting with various allergic or helminthic diseases. The sensitivity was assessed in patients exhibiting clinical and biological symptoms indicative of toxocariasis, serodiagnosis of which was made by the Western blot (WB; immunoblot) procedure that used the same antigen as that used in the sIgE ELISA but that detected specific IgG. The value of the sIgE ELISA for posttreatment follow-up was tested in two groups of patients: one group was treated with diethylcarbamazine; the other group was not treated with DEC. Results showed that the specificity and sensitivity of the sIgE ELISA were moderate. Thus, the sIgE ELISA appeared to be insufficient for properly ensuring the serodiagnosis of toxocariasis when it is used alone. However, sIgE ELISA might be an interesting complementary method for the detection of specific IgG. It was the only assay that was found to be positive in sera from some hypereosinophilic patients. sIgE ELISA values decreased significantly among the patients treated with DEC, indicating that this test would be useful for posttreatment follow-up assessment.

Antibodies, Helminth↗
PubMed1991

Application of the western blotting procedure for the immunodiagnosis of human toxocariasis.

To improve the immunodiagnosis of human toxocaral disease, a sensitive and specific assay using the Western blotting procedure (WB) with excretory-secretory antigens from Toxocara canis larvae (TES) was developed and compared with the standard enzyme-linked immunosorbent assay method (TES-ELISA) using the same antigens. We tested groups of sera from laboratory animals or patients presenting with toxocariasis or other helminthic diseases and a group of sera from people dwelling in an area endemic for toxocariasis who exhibited hypereosinophilia. Statistically, the WB assay correlated well with TES-ELISA, but the former was more specific for banding patterns corresponding to low-molecular-weight fractions, thus avoiding problems of cross-reactivity with sera infected with other helminthic diseases.

Animals↗