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Biomedical subjects

R Farkas

Publications and source records attributed to R Farkas.

13 recordsLinked to original sources

[Stress on the upper ankle joint in tennis-specific forms of movement].

The permanently increasing number of injuries in tennis, which are mainly located at the lower extremity, indicates that the internal load e.g. applicated to the ankle during certain movements might be a critical factor. In the present study the relationship between calculated ankle load and muscle forces appearing during tennis techniques performed in a laboratorial setup and some varying parameters such as playing surface, footwear and individual differences in movement structure were investigated. It could be shown that on a felt tennis floor due to its frictional characteristics the midfootstriking subject produced the highest ankle load, while the heelstriking technique reduces the load as well as the change of the playing surface to a floor with loose granulate. In addition it should be considered that external forces (e.g. gained from a force plate) must not be used as predictors for internal load. The presented data show that the model-aided load calculation (MALC) from two trials with similar ground reaction forces leads to completely different values of e.g. the ankle-joint force.

Ankle Injuries

Appearance of the T-cell marker CD8 on B chronic lymphatic leukemia cells in long-term cultures.

Long-term cultures of highly purified B chronic lymphatic leukemia cells (B-CLL), were established. The B-CLL lymphocytes acquired the CD8 T cell marker in long-term cultures while still retaining their surface immunoglobulins. In confirmation of previous results, the cultured B-CLL lymphocytes released factor(s) into the culture medium that suppressed the allogeneic mixed-lymphocyte reaction. No correlation was found between the appearance of the CD8 T cell surface marker and onset of suppressor activity.

Antigens, Surface

Long-term cultures of chronic lymphocytic leukemia B cells generate B suppressor cells.

B suppressor cells (Bs) were generated by stimulation with PHA-P or concanavalin A of B cells from peripheral blood of B-cell chronic lymphocytic leukemia (B-CLL) patients. They suppressed well allogeneic mixed lymphocyte reactions. Long-term colonies of B cells from B-CLL were established for up to 6 weeks in liquid media, with B cells acquiring properties of suppressor cells while being cultured. Two types of cultures were observed: cells either grew in compact multicellular colonies or formed diffuse monolayers. Cells that remained alive in cultures for a number of weeks, but did not multiply, did not develop suppressive characteristics. Bs cells retained their phenotypic markers in cultures. Mitosis and lymphoblasts, but no differentiation into plasma cells, were observed. PHA-P-generated Bs were cultured in long-term colonies, retaining their suppressor properties. The establishment of long-term cultures of B-CLL cells may facilitate a better understanding of the nature and characteristics of normal and leukemic B cells.

Aged

Generation of B suppressor cells by phytohaemagglutinin.

Activation by phytohaemagglutinin-P (PHA-P) of B cells from peripheral blood of healthy subjects generated B suppressor cells which inhibited allogeneic mixed lymphocyte reactions (MLR) and PHA-induced DNA synthesis. Their action was direct, that is, not mediated through the induction of T suppressor cells. The finding that B cells can be induced by PHA to become highly effective suppressor cells may have important clinical and experimental implications.

B-Lymphocytes

Decreased aminotransferase activity of serum and various tissues in the rat after cefazolin treatment.

Treatment of rats with cefazolin in vivo significantly suppressed activity of alanine and aspartate aminotransferases in serum and in the liver, brain, kidney, and heart. Simultaneous administration of pyridoxal further reduced enzyme activity except in the liver, where there was no change. Pyridoxal 5'-phosphate partly reversed the decreased enzyme activity in the serum, liver, and kidney, but did not return it to the amount observed in the control animals; enzyme activity remained suppressed in the brain and heart. The effect of cefazolin was dose related, but there was no sex-related difference. In contrast to its action on am-notransferase activity, cefazolin elicited no effect on alkaline phosphatase (pyridoxal-5'-phosphate hydrolase) in serum or on pyruvate carboxylase in the liver, heart, and kidney. Cefazolin exposed to the hepatic microsomal mixed-function oxidase system in vitro was partly converted into metabolites that inhibited serum alanine aminotransferase activity in vitro. The latter inhibition was reversed by the addition of pyridoxal 5'-phosphate.

Alanine Transaminase