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R Fidler

Publications and source records attributed to R Fidler.

11 recordsLinked to original sources
PubMed1989

[Isolated symplasts and stromal syncytial buds of the normal human placenta at 6 to 14 weeks of pregnancy].

Formation of isolated symplasts (IS) ("deported trophoblast", according to English nomenclature) and stromal syncytial buds (SSB) has been studied in 10 normal patients at pregnancy of 6-14 weeks by means of the indirect immunoperoxidase method. Monoclonal antibodies against beta-subunit of the human chorionic gonadotropin (beta-HCG), serial paraffin slices, stained with hematoxylin-eosin, semithin slices, stained with toluidin blue-pyronine and scanning electron microscopy have been used. A great amount of syncytiotrophoblastic processes and IS, analogous by their structure to syncytiotrophoblast are revealed. Presence of beta-HCG in the syncytiotrophoblast and its absence in the cytotrophoblast are proved. Concentration of beta-HCG in the IS is higher than in the syncytiotrophoblast of the villi. Maximal concentration of beta-HCG in the SSB is described for the first time. The functional role of the IS and SSB is discussed in the system mother-placenta-fetus.

Cell Fusion↗
PubMed1989

[Obtaining of monoclonal antibodies to human growth hormone and their characteristics].

The aim of present study was to obtain the hybridomas producing monoclonal antibodies against human growth hormone (Mabs hGH), to investigate the properties of the obtained Mabs and the possibility of their application in immunoanalytical systems. Two hybridomas secreting Mabs against hGH and belonging to the IgGI subclass have been obtained. The cross-reactivity of the Mabs with structurally similar to hGH hormones (hGHbio, hPL, hPRL, bGH, bPRL, pPRL) using indirect IFA has been studied. It has been shown that Mabs hGHI and Mabs hGH2 are directed to common specific antigenic determinant i.e. they have the same epitope specificity and don't react with other structurally related hormones, i.-e. this determinant is unique for hGH. The obtained Mabs hGH2 would find application for determination hGH by immunochemical methods in fractions while the hormone isolation from pituitaries and hGH obtained recombinant DNA methodology. The development of immunosorbents on the basis Mabs hGH2 seems to be perspective. Application of this immunosorbent may give possibility to optimize hormone isolation process.

Animals↗
PubMed1988

[Monoclonal antibodies against the beta subunit of human chorionic gonadotropin].

The aim of investigation was creation of hybridomas, which produce monoclonal antibodies to the beta-subunit of human chorionic gonadotropin (beta-hCG), characterization of monoclonal antibodies, which necessary for hCG immunoassay in biological fluids, as an immunological methods of detection of early stage of pregnancy and choriocarcinoma. 4 hybridomas, producing monoclonal antibodies to-hCG of IgG1 isotype, were created. On the base of monoclonal antibodies, which produced by D2 hybridoma cell line, test-systems for RIA of hCG in blood serum and urine were elaborated. These test-systems can be used in medical practice for diagnosis of early stages of pregnancy and choriocarcinoma.

Animals↗
PubMed1983

A search for malignant cells in peritoneal fluid from patients with colorectal adenocarcinoma.

Cytologic examination of peritoneal fluid aids in staging ovarian malignancies and in selecting gastric adenocarcinoma patients for intraoperative adjuvant chemotherapy. Tissue culture of peritoneal fluid could be potentially valuable in confirming cell viability and establishing sensitivity to a variety of anti-cancer agents. It also could be a more sensitive diagnostic tool than cytologic examination alone. We obtained peritoneal fluid specimens from 29 patients at the time of celiotomy: 22 had colorectal adenocarcinoma and seven were controls. Cytologic examination on a portion of each specimen produced only one positive result for malignant cells. Tissue culture of the remainder of the specimens grew cells from all but one of the patients with colon cancer; however, their morphology was similar to the fibroblasts and mesothelial cells that grew from the seven controls. We speculate that these negative results stem from the absence or reduced number of tumor cells in the specimens. Without additional refinement of our methods of specimen collection and processing, cytologic examination and tissue culture of peritoneal fluid from patients with colorectal cancer are of minimal value. Nevertheless, we believe that, with the necessary refinements, they may eventually become invaluable in the management of these patients.

Adenocarcinoma↗
PubMed1977

Prolongation of survival of rat parathyroid allografts by enhancing serum and tissue culture.

To determine whether survival of allografted rat parathyroid tissue could be enhanced either by the administration of antidonor serum (enhancing serum) or by tissue culture of the graft, parathyroids from Lewis x Brown Norway F1 (LBNF1) rats were transplanted beneath the fascia and between hamstring muscle fibers of hypocalcemic parathyroidectomized Lewis recipients (serum calcium 4.3 +/- 0.13mg%--mean +/- SEM). A drop in serum calcium below 7.5 mg% indicated rejection. Seven group 1 rats that received isogeneic parathyroids recovered from their hypocalcemic state and remained normocalcemic indefinitely. Seven group 2 rats that received LBNF(1) parathyroids experienced a reduced mean maximal response (MMR) of calcium increase of 4.1 +/- 0.2 mg% and the grafts had a median survival time (MST) of 16.0 +/- 1.32 days. Group 3 rats that received 1 ml of enhancing serum by intraperiotoneal injection, cytotoxic titer 1:128, on alternate days for five doses experienced an MMR of 5.0 +/- 0.7 mg% and had an MST of 19.3 +/- 1.07 days. When enhancing serum, cytotoxic titer 1:256, was administered to group 4, the MST of the grafts was increased to 29.7 +/- 1.19 days. When LBNF(1) parathyroid tissue was preserved in tissue culture for 14 to 21 days and then transplanted into Lewis recipients, the MST was 22.5 +/- 1.48 days. The administration of enhancing serum to recipients of cultured parathyroids further extended graft survival, MST 57.5 +/- 2.32 days. In summary, survival of allografted parathyroid tissue can be prolonged by the administration of enchancing serum or tissue culture of the graft. When enhancing serum was administered to recipients of cultured allografted parathyroid tissue, survival was further increased.

Animals↗
PubMedDate not supplied

[Monoclonal antibodies to bovine prolactin interacting with human prolactin].

Two stable hybridomas producing antibodies (Mab 1 and Mab 2) to bovine prolactin and belonging to the IgG1 subclass have been prepared. The cross-reactivity of Mab 1 and Mab 2 with some structurally similar pituitary protein (human, pig, whale, rat prolactins, bovine and human somatotropins) using indirect immunoenzymatic assay, was studied. It has been shown that Mab 2 reacts specifically only with bovine prolactin whereas Mab 1 interacts with human prolactin and prolactins of different animals. The specificity of Mab 1 to human prolactin was confirmed by immunoradiodetection assay on nitrocellulose filters. The data obtained give evidence of the existence of at least two different sterically nonoverlapping epitopes: one of them is specific exclusively for bovine prolactin and the other one is common, i.e. extraspecific.

Animals↗
PubMedDate not supplied

[Expression of human growth hormone in cultured mouse fibroblasts].

The new system for the transfer and expression of foreign genes based on retroviral vectors pPS-neo, conferring neomycin resistance was constructed. The BALB/c mouse cell lines producing highly active human growth hormone (more than 7 micrograms/ml into culture medium) were constructed using these vectors. An antibody column was used to purify the growth hormone from cell culture medium. Possibilities of producers to be applied for gene therapy are discussed.

Animals↗