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R Filmon

Publications and source records attributed to R Filmon.

30 records · Page 2Linked to original sources
PubMed1984

Bone tissue in Paget's disease treated by ethane-1, hydroxy-1, 1 diphosphonate (EHDP). Structure, ultrastructure, and immunocytology.

Diphosphonates have been used in the management of Paget's disease of bone because of their experimentally demonstrated antiosteoclastic activity. Light microscopic examination of bone tissue from 13 patients with Paget's bone disease who were receiving (EHDP) treatment showed considerably reduced osteoclastic resorption accompanied by gradual replacement of irregular woven bone by lamellar tissue. Electron microscopic examination confirmed that pagetoid osteoclasts are morphologically abnormal cells and degenerate after EHDP treatment. EHDP does not affect the measles nucleocapsid-like inclusions in the osteoclast nuclei. Immunocytologic analysis showed that during EHDP treatment viral antigens of the measles type persist in the cytoplasm and nuclei of osteoclasts even when these cells are greatly altered. These findings support the hypothesis of a viral etiology in Paget's disease of bone.

Aged↗
PubMed1983

[Paget's bone disease. Preliminary serological study].

A serological study has been carried out in Paget's bone disease where the etiology still remains uncertain. Previous work on patients with the disease revealed specific osteoclast inclusions that could be linked to the presence of a virus of the paramyxovirus group. Conventional methods for exploring humoral immunity reveal no great differences in the concentration of antibodies against the various viral strains tested on sera from 46 patients with Paget's bone disease and from 46 paired controls. The viral origin of Paget's bone disease is reconsidered in the light of the results obtained. The eventuality of sub-threshold viral infection and the possible action of incomplete or defective virus leading to the chronic nature of the disease are discussed.

Aged↗
PubMed1983

[Paget's bone disease. Preliminary serological study].

A serological study has been carried out in Paget's bone disease where the etiology still remains uncertain. Previous work on patients with the disease revealed specific osteoclast inclusions that could be linked to the presence of a virus of the paramyxovirus group. Conventional methods for exploring humoral immunity reveal no great differences in the concentration of antibodies against the various viral strains tested on sera from 46 patients with Paget's bone disease and from 46 paired controls. The viral origin of Paget's bone disease is reconsidered in the light of the results obtained. The eventuality of sub-threshold viral infection and the possible action of incomplete or defective virus leading to the chronic nature of the disease are discussed.

Aged↗
PubMed1981

[Viral origin of Paget's disease of bone. Contribution of electron microscopy and immunocytology of the aetiological diagnosis (author's transl)].

Despite considerable research work the cause of Paget's disease of bone remains uncertain. In a study of bone tissue from 100 patients with Paget's disease electron microscopy demonstrated alterations of the cells involved in bone remodelling. Osteoblasts showed distinct signs of hyperactivity, and the fibrillar arrangement of the osteoid tissue they produce was irregular. Osteoclasts were very large with numerous nuclei, and their bone-resorption activity was abnormal. Microcylindric inclusions visible in the cytoplasma and nuclei were morphologically comparable to the nucleocapsids of measles virus observed in experimental infections or in human pathology. Immunocytology tests confirmed the presence in osteoclasts of an antigenic material belonging to the measles virus group and cross-reacting with it. The hypothesis of a viral origin for Paget's disease of bone is supported by the chronic course of the condition, the histopathology of bone tissue, the ultrastructural changes in osteoclasts and the immunological results. A virus of the measles virus group probably plays a major role in the etiology of the disease.

Animals↗
PubMed1981

Towards a viral etiology for Paget's disease of bone.

The etiology of Paget's disease of bone has long remained obscure. The recent discovery in electron microscopy of specific inclusions in the cytoplasm and nuclei of osteoclasts in tissue from patients with Paget's bone disease has been the starting point of investigations into a possible viral origin. The inclusions, made up of microcylinders, described by several authors as being found only in osteoclasts in Paget's bone disease, present a close morphological analogy with nucleocapsids of paramyxovirus of the measles group. Immunocytological methods have provided a complementary approach to the problem. It has been demonstrated that the osteoclasts in Paget's bone disease contain antigenic material which reacts positively with sera containing measles antibodies. Both the morphological and the immunocytological evidence is strongly in favour of a viral etiology for Paget's bone disease.

Humans↗
PubMed1980

Bone tissue in Paget's disease of bone. Ultrastructure and Immunocytology.

Ultrastructural observation in Paget's disease of bone clarify aspects of bone cells in bone tissue and demonstrate the presence of specific intranuclear inclusions composed of microcylinders in the osteoclasts. The morphologic analysis of these structures suggests an analogy with virus material of the measles group. Results obtained using indirect immunofluorescence and immunoperoxidase techniques lend further support to the hypothesis of a viral etiology in Paget's disease of bone.

Animals↗
PubMed1979

[Demonstration by immunofluorescence and immunoperoxidase of an antigen of the measles type in the osteoclasts of Paget's disease of bone].

The intra nuclear and intra cytoplasmic inclusions described in osteoclasts in PAGET's bone disease are morphologically similar to those observed in subacute sclerosing panencephalitis. Immunological techniques using different specific immune sera demonstrate the presence of an antigenic structure of viral origin in osteoclasts in PAGET's bone disease. A measles or a measles like virus is most likely to be involved and may play a role in the etiology of the disease.

Antigens, Viral↗
PubMed1975

[Osteoclastic inclusions in Paget's disease of bone].

An electron microscope study of 35 bone biopsies from patients with Paget's disease confirmed the constnat presence of intra-nuclear and intracytoplasmic inclusions, which seemed to be the most specific anomalies of the osteoclasts. The presence of these inclusions leads to their comparison with other inclusions described in certain affections for which the viral etiology is at present under discussion.

Biopsy↗