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Biomedical subjects

R Flos

Publications and source records attributed to R Flos.

14 recordsLinked to original sources
PubMed1992

Determination of textile dyes in organs of Oncorhynchus mykiss W. after in vitro exposure.

The quantitative determination in organs of Oncorhynchus mykiss W. of a premetallized dye (C.I. Acid Violet 66) and the azoic base (C.I. Acid Red 217), used in wool dyeing, is studied in this paper. UV-VIS Spectrophotometry was used for the chromatic quantification and atomic absorption spectrophotometry for the detection of the metal. The method described here is as satisfactory for the detection of some commercial dyes as the methods developed by ETAD (Ecological and Toxicological Association of the Dyestuffs Manufacturing Industry).

Animals↗
PubMed1991

Cadmium induction of metallothioneins in several dogfish organs.

Dogfish Scyliorhinus canicula were exposed to 50 mg/l Cd for 4 days for inducing metallothionein synthesis. Spleen, pancreas, kidney and gonads were dissected out and metallothionein presence was checked by means of gel filtration chromatography in Sephadex G-75 and sodium dodecyl sulphate polyacrylamide electrophoresis. In pancreas and kidney, a cadmium-binding protein with spectroscopical and electrophoretic properties similar to those of dogfish liver metallothionein was found. In the other organs, the existence of an analogue protein but at very low concentrations is feasible.

Animals↗
PubMed1987

Effects on dogfish haematology and liver composition after acute copper exposure.

1. Dogfish Scyliorhinus canicula was subjected to experimental acute copper pollution. At the lowest concentration no changes were recorded on haemoglobin levels whilst decreases of the erythrocyte number and haematocrit was found. 2. At higher concentrations near to the lethal doses, a general reduction of all blood parameters was observed. 3. Liver composition (protein, glycogen and lipid levels) was not affected by any copper concentration, over the duration of the experiments. 4. Results are discussed in relation to physiological responses of fish, acute concentration and metal action.

Animals↗
PubMed1987

Acute toxicity of copper to mediterranean dogfish.

Acute copper toxicity to dogfish (Scyliorhinus canicula) was determined. Median lethal concentrations (LC50) for 24 and 48 hr were respectively 16 and 4 mg/l. These results are compared with those for zinc in dogfish and with the values for copper toxicity in several teleosts obtained by other authors.

Animals↗
PubMed1986

Effects of confinement stress and additional zinc treatment on some blood parameters in the dogfish Scyliorhinus canicula.

Several blood parameters were studied in the dogfish Scyliorhinus canicula subjected to different confinement conditions (transport and maintenance) and zinc contamination. Confinement stress significantly decreased erythrocyte counts (RBCC), haematocrit (Ht), haemoglobin (Hb), leucocrit (Lt), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC). Mean corpuscular volume (MCV) did not change with this stress and plasma glucose concentration significantly increased. In fish subjected to confinement stress conditions, Zn treatment significantly decreased Hb, MCH and MCHC and plasma glucose concentration. Ht and MCV did not change and Lt and RBCC significantly increased. Results are discussed related to other stress effects and effects of zinc treatment on fish confined in non-stressful conditions.

Animals↗
PubMed1986

Metallothionein in the liver of the small lizard Podarcis muralis.

A cysteine-rich protein presenting optical and biochemical features typical of metallothionein and a similar amino acid composition was found in the liver of the small lizard Podarcis muralis. Animals were given either CdCl2 (0.8 mg Cd2+/kg body wt) or saline (NaCl 0.9%) by i.p. injection for 3 days. A second group of animals were injected with a single dose of [35S]cysteine plus CdCl2 or saline. Lizard MT contained Zn and Cu when injected with saline and also Cd when injected with CdCl2. Metallothionein induction by cadmium was demonstrated by radioactive labelling.

Amino Acids↗
PubMed1986

Dogfish metallothionein--I. Purification and characterization and comparison with rat metallothionein.

A low-molecular weight metal-binding protein has been found in the liver of the dogfish (Scyliorhinus canicula) in both control and cadmium-treated animals and in both male and female animals. This protein is present as Zn, Cu-protein in control and as Cd, Zn, Cu-protein cadmium-treated (both water and i.p. administration) animals. This hepatic protein was characterized as metallothionein from several properties related to mammalian metallothionein. A different response to cadmium administration between sexes is suggested.

Amino Acids↗
PubMed1986

Dogfish metallothionein--II. Electrophoretic studies and comparison with rat metallothionein.

A low-molecular weight metal-binding protein has been isolated and characterized as metallothionein in the liver of the dogfish (Scyliorhinus canicula). This protein is present in both male and female animals and both control and cadmium-treated (both water and i.p. administration) animals as revealed by SDS-PAGE studies. Dogfish metallothionein shows the same metal-dependent anomalous behaviour as rat metallothionein in SDS-PAGE. SDS-PAGE is a good method to identify a protein as metallothionein.

Animals↗
PubMed1985

Cd-, Zn-, Cu-binding protein in the elasmobranch Scyliorhinus canicula.

A Cd-, Zn-, Cu-binding protein was isolated from the liver of dogfish subjected to environmental experimental contamination (50 ppm Cd). It was also found in liver from untreated fish. This protein has a high absorption at 250 nm and a low absorption at 280 nm, suggesting a mercaptide bond and a lack of aromatic amino acids. The SDS-PAGE pattern and changes in absorption spectrum on adding Cd or lowering pH are found as for mouse MT. The protein contains Cu and Zn in control and Cu, Zn and Cd in treated fish. Cd levels in the protein are significantly higher in females and significantly increase with treatment duration. Copper levels decrease after 96 hr in males and 6 days in females.

Animals↗
PubMed1978

Stress simulation: ACTH and zinc effect on trace metals in liver and spleen.

Effect of ACTH and zinc acetate subcutaneous injection on the trace metals in liver and spleen was investigated in male Wistar rats. Iron, copper, zinc and manganese in liver, and iron, copper and zinc in spleen were analyzed by AAS method. The iron, copper and zinc levels in liver, and iron levels in spleen showed a significant decrease at 1-1 h 30 min after administration of 30 UI of ACTH but values returned to normality or showed slightly higher levels at 4 h 30 min-7 h 45 min. Zinc acetate injection only elicited an increase in liver and spleen zinc levels. Results are discussed in relation with results given in previous papers.

Adrenocorticotropic Hormone↗
PubMed1978

Stress simulation and iron metabolism: adrenaline administration.

The effect of stress simulation on Wistar male rats through adrenaline administration (subcutaneous injection, 0.5 mg/kg) was investigated. Parameters measured were: plasma iron, copper and zinc levels; caeruloplasmin concentration and total ferroxidasic activity in plasma; iron, copper, zinc and manganese levels in liver and iron; copper and zinc levels in spleen. No significant short term effect of adrenaline administration on any of these parameters was observed.

Animals↗