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Biomedical subjects

R Fuchs

Publications and source records attributed to R Fuchs.

At least 127 records · Page 7Linked to original sources
PubMed1984

Screening for ochratoxin A in blood by flow injection analysis.

A micromethod for ochratoxin A detection in human sera by flow injection technique is described. The method requires 50 microliter of sera, and it is designed to distinguish samples containing less than 10 ng ochratoxin A per ml. The method is based on fluorescence measurement following a simple extraction procedure for which very small amounts of chemicals are needed. Since the method is not confirmatory, all samples showing fluorescence above a certain intensity have to be reanalysed with some other method where a confirmation step in included. Because of the small amount of serum needed and the rapid procedure (less than 15 min), a large number of samples can be analysed very quickly. The method may therefore be applicable for large screening campaigns conducted to determine the presence of ochratoxin A in blood. This conclusion is based on 1675 samples and 147 standards analysed concurrently by the flow injection technique and an earlier published enzymic method. The method is also suitable for monitoring ochratoxin A levels in the blood of experimental animals.

Chromatography, High Pressure Liquid↗
PubMed1984

Conversion of ochratoxin C into ochratoxin A in vivo.

The conversion of ochratoxin C to ochratoxin A was studied in rats after oral and intravenous administration. The concentration of ochratoxin A in the blood as a function of time was the same after oral administration of equivalent amounts of either ochratoxin C or ochratoxin A. The maximum ochratoxin A concentrations were measured 60 min after administration. Given intravenously, ochratoxin C was also converted to ochratoxin A. Maximum concentrations were reached after 90 min. It is concluded that ochratoxin C is readily converted to ochratoxin A after both oral and intravenous administration. There is reason to believe that a comparable toxicity of the two toxins is based upon this conversion and that only interference with the biotransformation mechanisms may cause a difference in their toxicity.

Administration, Oral↗
PubMed1984

[Rationalization and limits of diagnostic and operative measures in invasive cholestasis diagnosis (percutaneous transhepatic cholangiography) in primary medical care].

In a case of jaundice PTC enables the surgeon to detect its mechanical cause without further delay. This method is especially helpful in smaller hospitals without CT or even sonographic equipment. Explorative laparotomy is no longer necessary. An immediate decision can be made whether to perform a palliative or curative procedure or only to install a percutaneous bile drainage.

Biliary Tract Neoplasms↗
PubMed1981

Improved criteria for admission to cardiac care units.

Patients requiring specialized cardiac care unit interventions (CCUIs) were identified from 414 consecutive admissions with known or suspected myocardial infarction (Ml). Cardiac care unit interventions included administration of lidocaine hydrochloride, atropine sulfate, sodium nitroprusside, or vasopressors; Swan-Ganz or arterial catheterization; insertion of temporary pacemaker; and electroshock. Almost all interventions occurred in a high-risk group that had one or more of three findings: (1) ongoing chest pain, (2) pulmonary rales, or (3) one or more ventricular premature contractions (VPCs) on 12-lead ECG. Of 306 high-risk patients, 41% received at least one CCUI, and 4% died in the CCU. In contrast, of the 108 low-risk patients with none of the three criteria, only 6% received any CCUI, and none died in the CCU. This study suggests that patients who do not have ongoing pain, congestive heart failure, of VPCs when first evaluated have a very low risk of early complications and may not require intensive care.

Coronary Care Units↗
PubMed1981

Phospholipid composition of chlorophyll-free mitochondria isolated via protoplasts from oat mesophyll cells.

Mitochondria were isolated from oat primary leaves via mesophyll protoplasts and subjected to phospholipid analysis. In mesophyll cells mitochondria account for only small proportions of cellular phospholipids (in the order of 5%) and proteins (in the order of 2%). Contamination by lipids from other membranes was insignificant as indicated by the absence or very low levels of chlorophyll, galactolipids and steryl glycosides. The absence of 3-trans-hexadecenoic acid in phosphatidylglycerol from mitochondria of green cells serves an an additional criterion of purity. The phospholipid mixture extracted from these mitochondria resembles phospholipids in mitochondria from non-green tissues regarding composition as well as fatty acid profiles. Therefore, mitochondria maintain a rather constant lipid profile and in contrast to plastids do not respond at this level to differences in the physiological status of their housing cell. Palmitic acid in mitochondrial phosphatidylcholine and phosphatidylethanolamine is primarily localized at the C-1 position of the glycerol moiety. Two enzymatic activities so far not described in mitochondria, formation of acylgalactosyl diacylglycerol and hydrolysis of acyl-CoA, were found in the purified mitochondrial fraction.

Chlorophyll↗
PubMed1980

[Staging of morbus Hodgkin by explorative laparotomy (author's transl)].

75 patients suffering from Hodgkin's disease underwent explorative laparotomy. This meant to correct the initial staging in a percentage of 34. There was an increase of postoperative complications in secondary laparotomies. In 4 our of 25 patients an unexpected involvement of the spleen could be demonstrated.

Adolescent↗