Biomedical subjects
Publications and source records attributed to R G Kinsky.
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Placental extracts obtained from CBA (H-2k) mice during syngeneic (CBA female X CBA male) pregnancy were evaluated for their capacity to deviate the immune reaction of CBA mice toward A/J(H-2a) immunizing spleen cells, as tested by alloantibody subclass formation and Sa 1 allograft accelerated rejection-or enhancement. The immunomodulatory activity appeared to be located in the soluble and in the insoluble extracts of placenta. The sodium deoxycholate (SDO) solubilized proteinic material, fractionated on Concanavalin A [Con A] and injected to CBA mice simultaneously with A/J spleen cells caused a suppression of the hemagglutinating titer and C-mediated cytotoxicity (IgG2). However, it also favoured antibody-mediated mast cell degranulation (IgG1) and Sa 1 allograft enhancement. The presence and role of IgG1 antibodies in both tumor enhancement and gestation has been described. It is concluded that placenta contains Con A-binding glycoproteins which are located in the membranes and released in soluble form. They exert their immunomodulatory effect in a way which might help the successful outcome of pregnancy as it helps to enhance the development of allografted sarcoma.
Allo-immune sera were prepared by injecting CBA mice with spleen or thymus cells, or by two consecutive skin grafts of A/Jax origin. When absorbed 6 to 8 times on glutaradahyde treated A/Jax red blood cells (RBC), these sera lost all their haemagglutinating activity (anti- "SD" antibodies, predominantly H-2D) While their cytotoxic activities on A/Jax lymphocytes (anti-LD antibodies, perdominantly Ia, H-2IC) were partially preserved. Antibodies (anti-"XD") eluted form A/Jax RBC treated with an 0.5 M NaCl--HCL glycincing activity of these preparations was tested using SaI (A/Jax) grafts in CBA mice. Our results suggest that SD antigens are the main target of the enhancing effect. Further results obtained with DBA/2 anti-EL4 (C57Bl/6) sera confirmed this interpretation. Experiments performed with anti-idiotype and anti-recognition structure sera using tumor allograft targets, did not reveal any clear cut effect. Their relevance in tumor enhancement appears therefore doubtful.
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Previous studies have demonstrated that anti-K/D antibodies obtained by specific absorption on and elution from relevant red blood cells were capable of inducing enhancement of tumour cells in allogeneic hosts. Anti-Ia antibodies were not found enhancing except when alloimmune sera prepared by skin grafts were used. In the present report, anti-H-2K and anti-H-2D or anti-H-2I sera were obtained in mouse recombinant strains, in order to obviate such absorption and elution procedures. It has been shown that SaI-tumour cells (H-2a, i.e. H-2k/d) can be enhanced in allogeneic recipients, CBA (H-2k) or C57BL/Ks (H-2d) by antisera directed towards H-2Dd or H-2Kk. Alloimmune sera directed towards H2i-coded antigens prepared by immunization with spleen cells had no enhancing activity on the same tumour allografts in the CBA-A/J strain combination anti-IC'D,Sd,Gd) but caused a transitory effect in the C57BL/Ks-A/J combination (anti-IA'K,IBk,IJk,IEk).
CBA females pregnant of A/J males were tested for the presence in their sera of antiidiotypic antibodies, capable of combining with antipaternal Ig determinants. Radioimmunoassay tests have shown that in comparison to females pregnant of isogeneic males, allopregnant sera presented values indicating the presence of an antiidiotypic reaction in five out of seven trials. The possible role of antiidiotypic reactions in the control of maternofoetal relationship is discussed.