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Biomedical subjects

R G Reid

Publications and source records attributed to R G Reid.

5 recordsLinked to original sources
PubMed1991

Determination of teicoplanin in plasma using microbore high-performance liquid chromatography and injection-generated gradients.

A reversed-phase isocratic high-performance liquid chromatographic method for the determination of total teicoplanin in plasma is reported. The method developed uses a bracketing injection technique in conjunction with large injection volumes on a 1 mm diameter column to form a limited injection-generated gradient. The chromatography yields adequate resolution among all the major components for individual quantitation and also allows quantitation of total teicoplanin in plasma using ultraviolet detection. Pretreatment is by solid-phase extraction which uses C8 Bond Elut cartridges and gives effective clean up from endogenous materials. The method offers a faster and simplified means to determine total teicoplanin in plasma than those previously reported, and has a detection limit of 50 ng/ml.

Anti-Bacterial Agents↗
PubMed1987

Chromatography of progesterone and its major metabolites in rat plasma using microbore high-performance liquid chromatography columns with conventional injection and detection systems.

A separation of progesterone and its metabolites 17 alpha-hydroxyprogesterone, 20 alpha-hydroxy-4-pregnen-3-one, androstenedione and testosterone by high-performance liquid chromatography with a ternary solvent system is demonstrated. It is found that using a 100 X 1 mm microbore column, higher sensitivity is obtained in the ultraviolet detection of these compounds by using conventionally sized flow cells despite the higher efficiency resulting from the use of a micro-flow cell. It is also shown that, when a non-eluting solvent is used for injection, large injection volumes do not reduce column efficiency, and a 14-fold increase in sensitivity is obtained with a 1-mm column in place of one 4.6 mm in diameter. Using solid-phase extraction and concentration of the sample as pretreatment, progesterone and its hydroxylated metabolites are determined in 0.5-ml samples of rat plasma. The progesterone levels are compared with those obtained by radioimmunoassay.

17-alpha-Hydroxyprogesterone↗
PubMed1976

Purification and radioimmunoassay of human alpha-1-fetoprotein: the effect of aggregates on the radioimmunoassay.

A simple method for the extraction of alpha1-fetoprotein from fetal serum in high yield and of sufficient purity for use as radio-iodinated tracer in a radioimmunoassay is described. The purified alpha1-fetoprotein formed aggregates during storage. The presence of 125I-labelled aggregates affected the double antibody radioimmunoassay by increasing the non-specific bound, resulting in decreases assay sensitivity. Freshly prepared 125I-labelled monomer alpha1-fetoprotein is prepared for each assay. The radioimmunoassay described is sensitive to between 100 and 200 pg per tube. Normal adult serum levels were found to be in the range from less than 1 mug/l to 16 mug/l.

Electrophoresis, Disc↗
PubMedDate not supplied

Multidrug assay method for antimalarials.

A general separation strategy, involving solid-phase extraction followed by reversed-phase ion-pairing HPLC with an organic counter ion for a set of 11 widely used antimalarial drugs and metabolites has been developed. The basis underlying the separation has been explored and work, including quantitative data, has been carried out on illustrative separations which form the basis of novel quantitative assays of groups of antimalarials which are relevant to current prophylaxis and treatment of malaria.

Antimalarials↗