Biomedical subjects
Publications and source records attributed to R G Williamson.
The diagnosis of pancreatic disease is often difficult because of its retroperitoneal location and the non-specific nature of abdominal pain. There is no one test which provides all the information needed, and a series of complementary tests are generally required. This article discusses the different modalities available for investigating the patient with pancreatic disease.
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Semen was collected from Single Comb White Leghorn roosters, diluted 1:4 with Beltsville Poultry Semen Extender at 35 degrees C and cooled at various rates to 5 degrees C. DMSO was added to the semen between 10 and 120 min after ejaculation at temperatures between 15 and 5 degrees C, respectively. Following the addition of DMSO, the semen was allowed to equilibrate for 2 h in a 5 degrees C environment. The semen was then frozen at 1 degree C per min from 5 degrees C to - 20 degrees C, transferred into liquid nitrogen vapour for 4 to 10 min and then immersed in liquid nitrogen for 4 to 60 days. The thawed semen was inseminated on two consecutive days and fertility was calculated during 5 and 7 days commencing on the second day after the last insemination. In general, fertility was unaffected by the rate of cooling and the temperature at which the DMSO was added. In one trial, however, the fertilizing capacity was significantly greater if the DMSO was added within 45 min when the temperature of the semen was 15 degrees C. Approximately 42 to 71 p. 100 of eggs laid by hens during the 5 day period after the second insemination were fertile regardless of the method of cooling or the temperature of the semen when the DMSO was added. The motility of the ejaculates after thawing and before insemination varied between 15 and 55 p. 100 and this measure of physiological quality was a poor indicator of fertilizing capacity. Embryonic mortality was unaffected by the cooling and freezing procedure and hatchability of fertile eggs was not significantly different for the hens inseminated with either fresh or frozen semen.
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Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.