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R Giustolisi

Publications and source records attributed to R Giustolisi.

At least 55 records · Page 3Linked to original sources
PubMed1984

T-subset abnormalities in thalassaemia intermedia: possible evidence for a thymus functional deficiency.

Peripheral blood T-lymphocyte subsets, evaluated by means of a series of monoclonal antibodies, were assessed in 14 patients affected by thalassaemia intermedia, 7 of them previously splenectomized. A significant reduction of T+4 cells ('helper' T cells) was found in almost all patients, whereas T+8 cells ('suppressor/cytotoxic') showed a marked increase only in splenectomized subjects. Together with these quantitative T-subset abnormalities, which seemed to be partly affected by either splenectomy or high serum iron levels, an unusual circulating T-cell subpopulation labelled by T6 monoclonal antibody was detected in all patients. Complete disappearance of T+6 cells ('thymocyte-like' T lymphocytes) and normalization of the T4/T8 ratio was observed after 'in vitro' incubation of patient's lymphocytes with a crude thymus extract (Thymostimulin). This would suggest the presence of a so far unreported thymus-dependent defect of T-lymphocyte phenotypic maturation occurring in thalassaemia intermedia.

Adolescent↗
PubMed1983

[Fc gamma+ T-lymphocytes and depressed polyclonal immunoglobulin synthesis in multiple myeloma].

In the present study the effects on the polyclonal Ig synthesis of peripheral MM T gamma and T non-gamma cell fractions when cocultured with autologous and normal allogenic B-lymphocytes have been reported. Five untreated MM patients (three IgG-kappa, two IgG-lambda) were studied employing a five-day PWM-stimulated co-culture technique, in which the percentage of cells with detectable amounts of cytoplasmic Ig was evaluated by direct immunofluorescence. Our results indicated that depressed polyclonal Ig synthesis in human MM could be ascribed not only to an increased "suppressive" activity of T gamma cells but also to an impaired "inducer" function of T non-gamma lymphocytes and to an intrinsic B-lymphocyte disfunction as well. In addition, it was noteworthy that MM T gamma cells showed on normal Ig synthesis a selective suppressive effect mainly for those Ig chains corresponding to the monoclonal Ig which characterizes the own myeloma type. The significance of this datum, so far not yet reported, remains to be established.

Humans↗
PubMed1982

'Mu' heavy chain type 'non-excretory' myeloma.

An unusual case of 'non-excretory' myeloma is described in which, using immunofluorescence, only 'mu' heavy chains were detected in almost all bone marrow plasma-cell cytoplasm. Cytoplasmic light chains were completely lacking, and neither monoclonal whole immunoglobulin (Ig) nor free heavy nor light chains were detected in serum and urine, although the clinical and morphological features showed the classical pattern of myeloma. The possible mechanism which could play a role in the disturbance of the Ig-chain secretion observed in this case is discussed.

Aged↗
PubMed1980

Radioisotopic microassay of adenosine deaminase in human lymphocytes.

A radioisotopic method for a rapid assay of adenosine deaminase in human lymphocytes is proposed here. [2-3H] adenosine, as substrate, has been employed for the enzymatic assay. The products of reaction have been resolved by thin layer chromatography on PEI cellulose. The plates were developed with distilled water and inosine spots absorbing in the U.V. were eluted with 0.1 N HCl. The eluates obtained from the inosine spots were employed for radioactive measurements.

Adenosine↗