[Semimicromethod and screening test for determination of ALA in urine].
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Biomedical subjects
Publications and source records attributed to R Grisler.
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In order to verify if the method for the titration of salivary secretory IgA (s-IgA) by the technical of radial immunodiffusion is trustworthy, have been carried out the precision tests in the series and among the series in two saliva samples with very different s-IgA concentrations, and precision and accuracy tests in the series and among the series using a solution with a known titre of IgA. The results of the first battery tests have shown a precision ranging from 93.04 to 95.43%. The results of the tests of the second battery have shown a precision ranging about 96% and an accuracy about 97%. The tested method is, therefore, sufficiently trustworthy.
A simple enzymatic colorimetric procedure for the total serum bile acids determination was evaluated. In 518 normal patients values of statistical analysis ranged from 0.60 to 7.75 mumol/l (mean = 3.38 and S.D. = 2.00).
The simple Sclavo test based on succinyldicholine hydrolysis and on the following reaction with 5,5'-dithio-bis-2-nitrobenzoic acid for detection of the atypical patterns of serum cholinesterase has been investigated. A yellow staining appears but in presence of the atypical forms of the enzyme. Analysis of 4055 serum samples by the 430 Selective Analyzer Sclavo allowed the identification of one atypical homozygous patient. Prolonged apnoea induced by use of succinyldicholine as a neuromuscular blocking agent during anaesthesia may be avoided by use of this test.
A semiautomatic method for the determination of urine iron during treatment with deferoxamine B has been evaluated: samples were diluted 1:10 with a TCA 10% and thioglycolic acid 1.1% solution and processed after centrifugation by the aca III discrete clinical analyzer Du Pont. In this way an overestimation of values depending on the turbidity caused by proteins is avoided and the bad smell is limited thanks to the very low concentration of the thioglycolic acid solution. A good correlation with the atomic absorption spectrophotometry, specially for values less than or equal to 20 mg/l, was observed examining urine samples of 150 patients treated with deferoxamine B.
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