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R Guinet

Publications and source records attributed to R Guinet.

53 records · Page 3Linked to original sources
PubMed1983

[Antigenic detection and identification of Haemophilus influenzae and quantitative immunoelectrophoresis].

A one-step polyvalent rocket-line immunoelectrophoresis (PRLIE) for antigenic detection and identification of Haemophilus influenzae is described. A standard pattern of six lines corresponding to each individual antigen-antibody system has been established. The polyvalent antigen was an adequate mixture of each serotype antigen and the polyvalent antiserum was a pool of the six monospecific antiserum raised in rabbits (Institut Pasteur de Lyon). Each line has been identified with six reference individual antigens in PRLIE. The resulting sum pattern of rockets standing on the corresponding lines permits a direct characterization of the antigen content in a number of samples by means of a known line spectrum. The method is simple, reproducible, inexpensive (the polyvalent antiserum was used at 1% in the antibody-containing gel) and allowed quantitative determination.

Antigens, Bacterial↗
PubMed1982

Serological grouping of streptococci: a collective evaluation in six laboratories of three rapid methods.

Three serological methods for streptococci grouping: latex agglutination after pronase extraction (LAP), latex agglutination after nitrous acid extraction (LAN) and counterimmunoelectrophoresis after nitrous acid extraction (CIEN) were evaluated in six laboratories with 310 strains of streptococci. To reduce the possible influence of a particular laboratory, each serological procedure was performed in two different laboratories with identical protocols and strains. Between the three procedures, a total agreement of 81.3% with six identical results was obtained. Since only one discordant result was obtained in 12.2% (CIEN 4.8%-LAN 5.8%-LAP 1.6%) and two discordant results with two different serological methods in 2.6%, it could be concluded that agreement between the three procedures was 96%. For B (99 strains tested) and D(100 strains tested) groups, total agreements of respectively 95% and 92% were achieved. In conclusion, LAP, LAN, and CIEN gave equivalent results for streptococci grouping, but LAN was the most simple needing no heating, centrifugation, or electrophoresis equipment and lacking cross-reactivity of group C antibody.

Counterimmunoelectrophoresis↗
PubMed1982

Antigen overlay technique for line immunoelectrophoresis and crossed-line immunoelectrophoresis of thermolabile antigens.

A simple antigen overlay technique for line immunoelectrophoresis and crossed-line immunoelectrophoresis is described. A blind gel is overlaid with antigen-impregnated filter paper instead to mix antigens with molten agarose and to pour them in a uniform sample gel. This makes it easier in line and crossed-line immunoelectrophoresis comparison of different antigen samples, simplifying technique as compared with the conventional method. Moreover, this technique is designated to obtain reproducible patterns from thermolabile proteins since they are unaffected by being heated.

Animals↗
PubMed1981

[Repartition of Haemophilus influenzae biotypes responsible of purulent meningitis. (Study of 50 strains) (author's transl)].

The repartition of the Haemophilus influenzae biotypes responsible of meningitis in world were reviewed. In Senegal the vast majority of strains of Haemophilus influenzae isolated from CSF belonged to biotype I (94%), the biotypes II and IV were rarely encountered (respectively 4% and 2%). The biotypes of repartition in Africa is similar to this encountered in other world areas.

Haemophilus influenzae↗
PubMed1980

[Quantitative immunoelectrophoretic methods applied to the immunochemistry of Candida].

Cytoplasmic soluble extracts of yeasts were compared by quantitative immunoelectrophoretic methods. Comparative studies of Candida albicans and Candida tropicalis permitted to confirm the antigenic complexity of fungi and the existence of important cross-reacting antigens between this two yeast, to demonstrate the specificity of the Candida tropicalis cell-wall antiserum and the presence of soluble cell-wall constituents in cytoplasmic yeast extracts. Crossed-line immunoelectrophoresis with and without absorption of antibodies in situ demonstrated four specific antigens unique to Candida albicans group A cytoplasmic extract not shared with the group B. The other procedures used were not sufficient for identification, since the resulting patterns were confusingly complex.

Animals↗
PubMed1978

[Anti-Candida albicans IgM antibody: correlation between immunofluorescence and ultracentrifugation (author's transl)].

The indirect fluorescent antibody (IFA) technique for specific IgM anti-Candida albicans was applied to 158 sera and compared with sucrose density gradient centrifugation. For detection of specific IgM results of both methods were in agreement for 157 sera (99.36%). Specific IgM titers determined with IFA on unfractionated sera followed those found on fractionated sera. The results show that specific IgM anti-C. albicans can be identified and titrated by the immunofluorescent technique.

Antibodies, Fungal↗
PubMedDate not supplied

Treponemal antigens detected by immunoblotting with serum and CSF antibodies of neurosyphilitic patients.

Treponemal-antigen-eliciting antibodies in neurosyphilis were investigated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis immunoblotting with 17 sera and cerebrospinal fluid (CSF) (16 pairs) from 10 neurosyphilitic patients. Sera and CSF detected identical proteins. IgG antibodies in sera and CSF mainly revealed Treponema pallidum proteins of MW 48, 45, 38 and 37 Kd, and T. phagedenis proteins of MW 59, 54, 41, 40, 35 and 33 Kd. Few proteins were detected by IgM antibodies. Although no particular protein elicited antibodies specific for neurosyphilis, the immunoblot detection of antibodies to T. pallidum or T. phagedenis antigens in CSF or sera should be useful as a diagnostic tool for neurosyphilis.

Antibodies, Bacterial↗
PubMedDate not supplied

[Fast identification of Streptococcus pyogenes serotype M.12 in throat swabs by an improvised coagglutination technique].

A rapid procedure for identification of Streptococcus pyogenes serotype M. 12 directly in throat swabs, is reported and compared with standard culture method on blood agar plates and typing of group A Streptococci isolated, with double gel immuno-diffusion. This procedure consist of chlorhydric acid extraction of swabs and testing of the extract towards specific M. 12 protein serum using extemporaneous coagglutination technique. We have tested 1100 throat swabs, with this procedure and with standard culture procedure. Identification of group A Streptococci serotype M. 12 with reported method is obtainable within 30 to 45 minutes of receipt of the clinical specimen. This method is easy to perform, with a sensitivity and a specificity respectively: 89.7% and 98.8%.

Agglutination Tests↗
PubMedDate not supplied

[Specific soluble cell-wall antigens of "Candida tropicalis" demonstrated by crossed immunoelectrophoresis (author's transl)].

In the present study, soluble cytoplasmic extracts of Candida albicans and C. tropicalis were prepared and showed respectively 67 and 47 precipitates in crossed immunoelectrophoresis performed with a commerical goat anti-C. albicans immunserum. Purified cell walls of C. tropicalis have been prepared, and corresponding antiserum was got in rabbits. Although important cross-reacting antigens exist between soluble cytoplasmic extracts of C. albicans and C. tropicalis, the C. tropicalis cell wall antiserum precipitated antigens in crossed immunoelectrophoresis only in C. tropicalis soluble cytoplasmic extracts. The present study: - confirms that C. albicans shared 70% of the antigens with C. tropicalis; - demonstrates that soluble cytoplasmic antigens prepared by homogenization of yeasts contain cell wall constituents; - allows us to prepare a specific immune serum for C. tropicalis.

Animals↗