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R Gulati

Publications and source records attributed to R Gulati.

5 recordsLinked to original sources

Generation of a nested series of interstitial deletions in yeast artificial chromosomes carrying human DNA.

We have generated a nested series of interstitial deletions in a fragment of human X chromosome-derived DNA cloned into a yeast artificial chromosome (YAC) vector. A yeast strain carrying the YAC was transformed with a linear recombination substrate containing at one end a sequence that is uniquely represented on the YAC and at the other end a truncated long interspersed repetitive element (LINE 1, or L1). Homologous recombination between the YAC and the input DNA resulted in a nested series of interstitial deletions, the largest of which was 500 kilobases. In combination with terminal deletions that can be generated through homologous recombination, the interstitial deletions are useful for mapping and studying gene structure-function relationships.

Chromosome Deletion

Calcium channel blockers and terbutaline--a positive interaction.

The role of two calcium channel blockers, nifedipine and verapamil was investigated in patients of bronchial asthma. Both the drugs given orally produced an insignificant rise in PEFR, FEF25-75% and FEV1. Given along with terbutaline, however, these drugs produced significantly greater increase in PEFR, FEF25-75% and FEV1 as compared to patients receiving terbutaline alone. The two calcium channel blockers also inhibited the terbutaline induced tremors and palpitation.

Adult