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R H Dennin

Publications and source records attributed to R H Dennin.

29 records · Page 2Linked to original sources
PubMed1985

[Herpes simplex infection after kidney transplantation under immunosuppression by cyclosporin. Diagnostic and therapeutic experiences].

Incidence and course of herpes simplex infections was determined prospectively in 22 patients who had a kidney transplant and were treated with cyclosporin. In addition to clinical findings, serial studies were undertaken of throat washings for herpes simplex virus in cell culture, as well as of patient sera for herpes-specific IgG and IgM antibodies. There were 13 clinically manifest infections, 12 of them localized, while one had dissemination with necrotizing retinitis. Virus demonstration was successful in all cases in which virostatic drugs had not yet been used. Asymptomatic virus excretion was noted in three cases. Significant IgG titre rise occurred in six of the 13 cases, but a positive IgM titre in only two. Acyclovir proved to be an effective virostatic drug with few side effects. The outcome in the localized infections was favourable, but in the disseminated one residual defects remained.

Acyclovir↗
PubMed1985

[Modification of the incidence and course of CMV infections following kidney transplantation by passive immunization--initial experiences with a hyperimmunoglobulin].

In 45 recipients of a renal transplant the CMV-antibody titer was measured preoperatively (ELISA method). If possible, the donors were examined likewise. All seronegative recipients of grafts of a seropositive donor were immunized passively with a CMV-hyperimmunoglobulin for 6 months (2 ml/kg bw in 3 weeks intervals). 7 patients showed that constellation, and they were treated. 4 of them demonstrated neither serological nor clinical signs of a CMV-infection at any time. In 3 patients an infection was found serologically, but only 2 showed concomitant clinical symptoms. No serious complications (pneumonia etc.) were seen. One year later all patients are doing well with a functioning graft. For this reason we think the passive immunization of a seronegative recipient of a graft from a seropositive or unexamined donor to be advisable.

Adult↗
PubMed1984

[Serologic diagnosis of cytomegalic inclusion disease using the Elisa technic in kidney transplant patients].

During the first six months after transplantation cytomegalovirus-(CMV-)specific serum IgM and IgG antibody titres were determined regularly with the Elisa technique in 25 renal transplant recipients and compared with results of the complement binding reaction (CBR). Active CMV infection was diagnosed in six patients: two primary infections and four reactivations. Titres of 1 : greater than 40 were considered positive. IgG titre increases of up to 1 : 20 000 and of 1 : 4000 for IgM were observed. IgM titres of 1 : greater than 200 are suspicious of active CMV infection, four-fold titre increases establish the diagnosis. Such IgM titres were accompanied by clinical symptoms of active CMV infection. The Elisa technique is a reliable method from the first week of disease for early demonstration of CMV infection. The prognostically relevant differentiation between primary CMV infection and reactivation of latent disease is possible using the IgG/IgM antibody titre relations. IgM titres indicate cure or persistence of infection. CBR with CMV antigen is of no use for diagnosis.

Adult↗
PubMed1984

[Manifest chickenpox reinfection in pregnancy with low varicella antibody titer?].

The report concerns a case of an obvious varicella reinfection during pregnancy and the course of varicella-IgG and -IgM titers, respectively. By means of early diagnosis with an enzyme-linked immunosorbent assay (Elisa) and immediate application of a high - titered commercial varicella-zoster immunoglobuline, an infection of the foetus was probably prevented.

Adult↗
PubMed1983

[Evaluation of the effects of active rubella vaccination. Comparative studies of the evaluation of seroconversion using the ELISA technic and the hemagglutination inhibition test].

31 women were vaccinated with attenuated rubella-virus (RV) strains. According to the prevaccinal antibody status they were grouped as follows: I: HIT 1: less than 10, RV-specific IgG 1: less than 20; II: HIT 1: less than 10, RV-specific IgG 1: greater than 20; III: HIT 1: greater than or equal to 10, RV-specific IgG 1: greater than 30. The postvaccinal geometric mean titers for RV-ELISA-IgG were somewhat higher in group II compared to group I, eventually because of a weak booster effect based on low level prevaccinal RV-antibody titers--but the difference is not significant for 5%. The difference between RV-ELISA-IgG in group II and group I is significant. Within group II there is a low titer rise upon vaccination although elevated prevaccinal titers were already present. The quotient "RV-ELISA-IgG-titer: HIT-titer" is in the range of greater than or equal to 23:1 for the postvaccinal period (8-10 weeks after vaccination) compared to about 8:1 resp. in stationary sera of reconvalescent individuals. These quotients could be helpful for interpretation of suspicious elevated titers during pregnancy.

Antibodies, Viral↗
PubMed1982

[Determination of cytomegalo-, herpes simplex- and rubellavirus-antibodies resp. during perinatal period by Elisa-technique (author's transl)].

Cytomegalovirus (CMV)-infections attract increased attention among viral infections in the pre- und perinatal period resp. The decreasing cell mediated immunity by the end of pregnancy may favour conditions for primary infections or reactivations with CMV. With the Elisa-technique we looked for CMV-specific antibody status in the perinatal period with special respect to striking titers. The same serum samples were screened for antibody status to Herpes simplex virus (HSV) type 1 specific IgG and rubellavirus specific IgG resp. 67% were positive for CMV-IgG, 90% for HSV-type 1-IgG and 93.5% for rubellavirus IgG. CMV-specific IgM antibodies were found in 3.6% but only 1.5% were suspicious for recent CMV-infections according to higher CMV specific IgM titers.

Adult↗
PubMed1981

[Serologic rubella diagnosis. The determination of minimal rubella antibody levels].

75 serum specimens from different individuals protocolled negative (1 : less than 10) by routine rubella-hemagglutination inhibition test were found to contain rubella specific antibodies by means of a commercial rubella-ELISA-test kit in 38,7%. From the 3 groups assayed: pregnant women, routine medical inspection of applicants and medical inspection of children were positive: 19%, 33%, 47%, respectively.

Antibodies, Viral↗
PubMed1979

DNA of free and complexed origin in human plasma: concentration and length distribution.

The DNA content in plasma of five healthy human individuals has been estimated. Structures ascertained by electron microscopy have been identified as double-stranded DNA. Depending on the isolation procedure applied the DNA molecules found may originate from free and/or complexed forms of DNA. The lengths range from less than 0.2 microns up to about 13 microns. DNA concentration is in the range of 3 to 11 ng/ml.

Centrifugation, Density Gradient↗
PubMed1978

[Rotavirus as a causative agent of infantile gastroenteritis. Diagnosis and epidemiology (author's transl)].

Some years ago it was not possible in spite of intensive bacteriological and virological work on the etiology of acute infantile gastroenteritis to find any agent that may readily be linked to this disease. Since five years now early sporadic reports about virus-like particles have proved worldwide to be correct and in the meantime have substantially been supported by new findings in that these particles are the main causative agent of infantile gastroenteritis. As part of our electron-microscopic studies some morphological characteristics are shown. The physico-chemical as well as structural properties of the new virus apparently justify its classification into the family of the reoviruses. Rotavirus positive stool specimens were found in 46% of the material sent in by paediatric clinics.

Child↗