Biomedical subjects

R H Goodwin

Publications and source records attributed to R H Goodwin.

3 recordsLinked to original sources

PubMed1978

Gypsy moth cell lines divergent in viral susceptibility. I. Culture and identification.

A series of cell lines unique in insect virus susceptibility pattern have been isolated from the ovaries of the gypsy moth (Lymantria dispar: Lepidoptera: Lymantriidae) on a synthetic medium with mammalian and avian serum supplementation. Growth curves showed the poorest growth occurring on peptone-based media with somewhat better growth on amino-acid-based media. The best growth was obtained with combined media. Serological study distinguished the present cell lines from one another and from cell lines derived from other insect species grown routinely in the same laboratory. Baculovirus susceptibility among the new lines varied from no response to a specific complete replication response upon challenge by the homologous (gypsy moth) nuclear polyhedrosis virus.

Cell Division↗

PubMed1977

The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae).

The history and characteristics of two cells lines developed from primary explants of pupal tissue from the insect, Spodoptera frugiperda (J.E. Smith), are described. One cell line, IPLB-SF 21, was developed with hemolymph-supplemented medium and has been maintained continuously on the medium. The second cell line, IPLB-SF-1254, was developed with a medium containing a combination of vertebrate sera plus hemolymph and was adapted to hemolyphn-free medium at the 6th passage. The IPLB-SF-21 cell line has a population doubling time of 26 to 30 hr; the doubling time of the IPLB-SF-1254 line is 36 hr. The chromosomal morphology and distribution was typical of other lepidopteran cell lines. Serological studies showed that both cell lines have at least one antigen which also is common is tissue antigens from pupae of Spodoptera frugiperda.

Animals↗

PubMedDate not supplied

Insect cell culture: improved media and methods for initiating attached cell lines from the Lepidoptera.

Several cell lines from the pupae of the noctuid moth species Spodoptera frugiperda, Heliothis zea, and Trichoplusia ni were isolated on a synthetic medium containing insect hemolymph and turkey serum. These lines were progressively adapted to improved media free of insect hemolymph but containing one or more of the following sera: turkey, chicken, and fetal calf. Primary culture tissue disruption was improved by substituting collagenase for trypsin. Primary culture survival was improved by controlling the total tissue volume per unit medium volum, and by the addition of glutathione to prevent melanization and to improve cell adherence to the substrate. Culture servival was also improved by heat treatment of sera, control of medium osmolality, and changes in the basal medium and serum supplementation. Some of these changes also resulted in improved growth giving higher maximal cell counts. Comparative cell growth on the various media was graphed and generation times given.

Blood↗