Biomedical subjects

R H Granda

Publications and source records attributed to R H Granda.

4 recordsLinked to original sources

PubMed1991

GABA immunoreactive axons and growth cones in the developing chicken optic nerve and tract.

Immunohistochemical studies of the chicken embryo optic tract using an antibody to gamma-aminobutyric acid (GABA) reveal that the tract is initially free of GABA immunoreactive axons. During the second week of incubation, GABA+ axons appear in the tract, chiasm, and optic nerve. The number of GABA+ axons in the optic nerve increases through E18, although few are recognizable after hatching. Detailed staining of GABA+ growth cones confirmed that virtually all the GABA+ axons in the optic nerve were growing toward the retina. Taken together, the findings suggest that the GABA+ axons in the chiasm and nerve are largely a transient extension of the GABA+ optic tract cells, the tectogeniculate projection, or both.

Animals↗

PubMed1991

Chicken optic tract cells showing GABA-like immunoreactivity: morphological and immunocytochemical studies.

A population of cells has been found in the chick optic tract and chiasm exhibiting GABA-like immunoreactivity (GABA+; Granda and Crossland, J. Comp. Neurol. 287:455-469, '89). It is not known, however, whether the cells are neurons. We have studied the GABA+ cells by using morphological and immunocytochemical methods. We found that there are more than 500 cells in each tract. At the light microscopic level, the cells possess processes resembling dendrites and axons. At the electron microscopic level, the organelle content of the cells is similar to that of neurons. The cells are immunoreactive with antibodies to MAP2 and neuron specific enolase, two proteins characteristic of neurons. Taken together the findings indicate that the GABA+ cells of the chick optic tract are neurons, perhaps similar to the interstitial neurons found in the white matter of other vertebrates.

Animals↗

PubMed1989

GABA-like immunoreactivity of neurons in the chicken diencephalon and mesencephalon.

The chick brain is a useful model system for studying the ontogeny and phylogeny of neural circuitry, especially that of the visual system. In this study the distribution of cells and processes showing GABA-like immunoreactivity (GABA+) in the diencephalon and mesencephalon of the posthatch chick was determined immunohistochemically with a polyclonal antibody to GABA and compared with the results of similar studies in mammals. Most of the small GABA+ cells were found in the chick visual centers such as the nucleus lateralis anterior, suprachiasmatic nucleus, ventral lateral geniculate, optic tract, dorsolateralis anterior pars lateralis, lentiformis mesencephali, ectomammillary nucleus, area pretectalis, and the optic tectum. Large GABA+ cells were found in the following nuclei: reticularis superior, posteroventralis thalami, subpretectalis, isthmi pars magnocellularis, interstitio-pretectosubpretectalis, mesencephalicus lateralis pars dorsalis. These large cell-containing nuclei receive projections from visual or auditory centers. GABA+ axons were found throughout the diencephalon and mesencephalon but were especially prominent in the ansa lenticularis, fasciculus medialis longitudinalis, and optic tract. The distribution of GABA+ cells in the chick is more widespread than in rodents and exhibits an increased association with the visual centers suggesting a correlation with the specialized visual requirements of the bird.

Animals↗

PubMed1988

Morphological plasticity in the chick ventral lateral geniculate nucleus: temporal parameters.

The retinogeniculate projection in the chick undergoes apparent augmentation following lesions in the optic tectum. Using autoradiographic tracing techniques we determined that the alteration of the retinal projection required a minimum of 4 days to be detected if tectal lesions were made at hatching and could be produced by lesions placed up to 1.5 years posthatch.

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