Further studies on the rhamnomannans and acidic rhamnomannans of Sporothrix schenckii and Ceratocystis stenoceras.
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Biomedical subjects
Publications and source records attributed to R H Haskins.
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Seven commercial inhibitors of sterol biosynthesis were tested for their effect upon vegatative growth and sexuality in Pythium acanthicum and Zygorhynchus moelleri. Five compounds (SKF 525, Chlorpropamide, Tolbutamide, Benzmalacene, AY 9944) inhibited both growth and sexuality of both fungi and two (SKF 7732 and SKF 7997) were ineffective. Pythium was more sensitive to the activity of the inhibitors than was Zygorhynchus. None of the compounds tested inhibited sexuality without similarly reducing vegetative growth.
Electron microscope studies were made of wall regeneration by Pythium protoplasts. Wall regeneration began with the formation of a loose network of fibrils on the surface of the protoplast followed by increase in density of the fibrillar mesh and deposition of granular mitrix material. The majority of the protoplasts did not develop beyond the loose fibrillar network stage, however a small percentage were able to complete wall formation and to form hyphal tubes. A clear zone of demarcation was visible between the fibrillar surface of the protoplast and the smooth surface at the base of the developing hyphal tube.
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m-Cresol has been isolated from culture filtrates of Valsa friesii. The yield after 10 days of growth on glucose-phytone broth was 160 mg/liter.
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A fungus of Pythium species survives high and low temperatures longer when a suitable sterol is added to the growth medium.