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Biomedical subjects

R H Persellin

Publications and source records attributed to R H Persellin.

At least 37 records · Page 2Linked to original sources
PubMed1979

Human polymorphonuclear leukocyte phagocytosis in pregnancy. Development of inhibition during gestation and recovery in the postpartum period.

The phagocytosis of S. aureus by normal human PMN leukocytes was inhibited by pregnancy serum. Control sera from normal adult nulliparous women, from men, and from cord blood all functioned normally in support of phagocytosis. However, particle ingestion was reduced significantly (p less than 0.001) when leukocytes were in 15% pregnancy serum obtained at term. To determine at what stage in pregnancy the inhibition of phagocytosis could first be detected, sera were obtained from multiple pregnant donors and pooled according to week of gestation. Significantly fewer bacteria were ingested in each of the serum pools obtained after week 16 of pregnancy and the inhibitory effect persisted through gestation. Following delivery, less inhibition was detected as early as 2 days post partum. Phagocytosis assays were performed in six matched maternal and cord serum pairs. Five of the six maternal sera showed inhibition of phagocytosis; one pregnancy and all cord sera functioned normally in support of bacterial ingestion by normal granulocytes. Since neutrophils are essential to the development of rheumatoid arthritis and certian other inflammatory disorders, the subsidence of these diseases during gestation and their exacerbation post partum could be related, at least in part, to the inhibitory effects of pregnancy serum on leukocyte functions.

Blood Proteins↗
PubMed1979

Cellular augmentation of polymorphonuclear leukocyte chemotaxis.

The density of neutrophils influences the number of cells that will respond to a chemoattractant, endotoxin-activated serium. When fewer than 3 x 10(5) polymorphonuclear leukocytes (PMN) were placed in the top compartment of a modified Boyden chemotaxis chamber, the cellular response was weak. Complete membrane penetration by activated neutrophils rarely was observed. When this number of PMN was exceeded, however, both the number of cells and the percentage of neutrophils responding to the leukoattractant increased. The density of cells required for effective chemotactic response to occur was such that intimate cell-to-cell contact was suggested. This indicated that PMN exerted a kinetic influence upon one another. Extracts of disrupted PMN induced an otherwise insufficient number of neutrophils to respond to the chemotactic stimulus. The active component was isolated in the cytoplasmic fraction (postcentrifugation, 100,000 x g, 60 min) of PMN, but was not present in other subcellular fractions. This cytoplasmic augmentor of chemotaxis (CACh) increased random mobility of neutrophils, but was not, itself, a chemotactic factor. These findings suggest that PMN cooperate in their response to a leukotactic stimulus.

Cell Count↗
PubMed1978

Distribution of HLA antigens in a Mexican--American population and a comparison with two similar populations from different geographical locales.

The frequencies of 32 HLA antigens of the A and B series were determined in a previously unstudied population of Mexican-Americans in South Texas. The phenotype and gene frequencies for this group are presented and are contrasted with those determined in studies of Mexican/Mexican--American populations from two different locales. The statistically significant differences observed emphasize the need for indigenous controls when evaluating racially heterogeneous populations for disease-associated HLA antigens.

California↗
PubMed1978

Costovertebral and costotransverse joint involvement in rheumatoid arthritis.

Lesions of the costovertebral (CV) and costotransverse (CT) joints are distinctly unusual in rheumatoid arthritis. The patient presented had dramatic changes in these joints with destruction, ankylosis, and bony overgrowth. This led to a moderate respiratory impairment and a distinctive radiological presentation.

Adult↗
PubMed1975

Acute rheumatic fever in adults.

Acute rheumatic fever (ARF) in 53 adults was characterized by a severe, febrile migratory polyarthritis involving primarily large joints in the lower extremities, with evidence of an antecedent streptococcal infection. Carditis, present in only eight (15%) of the adults, was mild and transient. The characteristic abnormality in laboratory findings was an erythrocyte sedimentation rate (Westergren) greater than 100 mm/hr. Response to high-dose aspirin therapy was prompt and dramatic in all patients. Mild andevanescent abnormalities of both renal function and hepatic function (not aspirin-mediated) were detected in 51% and 64%, respectively. A common disorder in San Antonio, ARF has distinctive symptoms. It can be readily diagnosed and promptly treated. In the adult, it is almost exclusively a syndrome of events severe but transient in the joints, and mild and transient in the heart, kidneys, and liver.

Acute Disease↗
PubMed1974

Effects of steroid hormones on human polymorphonuclear leukocyte lysosomes.

Lysosomal membrane stabilization has been proposed as a mechanism for the anti-inflammatory action of corticosteroid hormones. This hypothesis was based on studies with liver organelles. We studied the action of steroids on intact lysosomes isolated from human peripheral blood polymorphonuclear (PMN) leukocytes. Both androstenedione and progesterone, 10(-3)-10(-5) M, caused leakage of acid hydrolase markers from these organelles, thus resembling their effects on liver lysosomes. But none of the anti-inflammatory steroids tested protected organelle membranes from either detergent lysis (Triton X-100) or heat incubation (37 degrees C, 90 min). Hydrocortisone (HC), HC sodium succinate, HC acetate, HC hemisuccinate, prednisone, and dexamethasone were without detectable stabilizing activity at concentrations of 10(-3)-5 x 10(-8) M. Release of the lysosomal marker, beta-glucuronidase, was not retarded by any of the compounds studied. In addition, PMN leukocyte lysosomes isolated from human volunteers receiving prednisolone were not more stable than control organelles, nor did serum from steroid-treated humans protect intact lysosomes from detergent lysis. Variations in cholesterol and phospholipid contents of liver and PMN leukocyte lysosome membranes could possibly account for the different reactivity to corticosteroids observed. We believe that the anti-inflammatory activity of adrenal corticosteroids can best be explained by their inhibitory effects on cellular metabolism rather than by their direct interaction with lysosomal membranes.

Acid Phosphatase↗