Biomedical subjects

R H Sawyer

Publications and source records attributed to R H Sawyer.

At least 19 recordsLinked to original sources

PubMed1996

Dorsal dermis of the scaleless (sc/sc) embryo directs normal feather pattern formation until day 8 of development.

We have examined the ability of the scaleless (sc/sc) backskin dermis (6 to 16 days of incubation) to regulate pattern formation using the presumptive scutate scale epidermis from 11-day normal embryos as the responding tissue. Prior to 8 days of incubation the sc/sc backskin dermis is able to induce hexagonally patterned and uniformly oriented feather germs in normal epidermis. This ability is lost during day 8 and follows a central to lateral gradient. Such gradients are characteristic of normal feather development in the spinal tract. We discuss the change in the inductive ability of the sc/sc dermis in relation to the stabilization of the feather pattern, which occurs all at once throughout the dorsal dermis at 7.5-8 days of development. After day 8 until day 10, the sc/sc backskin dermis only supports the formation of sporadic, unpatterned feather germs; thereafter it will not support feather formation.

Animals↗

PubMed1995

Histidine-rich protein B of embryonic feathers is present in the transient embryonic layers of scutate scales.

Based on its amino acid composition and N-terminal sequence, a polypeptide (HRP-B) has been identified as a member of the avian histidine-rich protein (HRP) family. An antiserum against HRP-B has been used to localize this polypeptide in developing feathers and scales of chick embryos. HRP-B was first detectable in the barb ridge cells of feathers at 13 days of incubation and progressively appeared in the distal/proximal and peripheral/central gradients observed previously for the feather-type beta keratins in developing feathers. The HRP-B polypeptide was detected only in the embryonic layers of scutate scales. It first appeared at 16 days of incubation and was not found in the differentiated beta strata of these scales. At no time during the development of reticulate scales or apteric skin regions did the epidermal cells or cells of the embryonic layers express HRP-B. The transient expression of HRP-B by the embryonic layers of the scutate scale epidermis is discussed in light of the feather-forming potential of the presumptive epidermis of the scutate scale-forming region.

Amino Acid Sequence↗

PubMed1995

Domiciliary radiography: an important service?

General practitioners' (GP) perceptions, comparative costs and potential influence on patient management of a domiciliary radiography service were reviewed. A postal questionnaire was sent to 159 local GPs, 130 of whom responded. Seventy-one per cent of respondents had requested domiciliary radiography in the previous year and 79% felt loss of the service could potentially be detrimental. Although 98% recognized that a chest X-ray was a suitable domiciliary radiography examination, 6% thought that a barium swallow could be adequately carried out with domiciliary equipment. Forty-nine per cent perceived a need for a domiciliary ultrasound service. Sixty-three per cent believed that domiciliary radiography was cheaper than return ambulance transport with departmental examination, although estimates showed a domiciliary visit to cost 82 pounds versus 60 pounds for a departmental visit. A retrospective review of 50 consecutive domiciliary radiography examinations showed that elderly and immobile patients were imaged most frequently. Malignancy was diagnosed in 14%, infection in 26% and a fracture in 14%. Overall there was an anticipated management change in 50% of patients.

Adult↗

PubMed1994

Role of epidermal-dermal tissue interactions in regulating tenascin expression during development of the chick scutate scale.

During normal chicken development tenascin begins to accumulate in the dermis of anterior metatarsal skin at the time of scutate scale ridge formation, and is localized in a distinct pattern along the outer scale surface. Anterior metatarsal skin from scaleless (sc/sc) embryos, which do not form scutate scales, begins to accumulate tenascin 4 days later than normal skin. This study shows that normal and scaleless anterior metatarsal dermis accumulate the same tenascin isoforms and undergo the same isoform changes in the post-hatch period, but there is less tenascin accumulated in scaleless dermis and there is no pattern to its distribution. In both normal and scaleless anterior metatarsal skin, tenascin mRNA is localized in the dermis and is distributed in the same way as the protein. Thus, scaleless skin is defective in the ability to accumulate appropriate amounts of tenascin and to maintain the tenascin in the patterned manner of normal. Recombinant skin cultures show that epidermal-dermal interactions are required for tenascin accumulation. The dermis specifies the way that tenascin is organized, but interaction with epidermis is required to maintain this organization. The epidermal role appears to be permissive because in heterotypic recombinants, neither scaleless anterior metatarsal epidermis nor normal footpad epidermis changes the way that tenascin appears in the normal anterior metatarsal dermis; and in reciprocal recombinants, normal anterior metatarsal epidermis does not change the way tenascin is accumulated in either scaleless anterior metatarsal dermis or normal footpad dermis.

Animals↗

PubMed1994

Pattern formation in chick feather development: distribution of beta 1-integrin in normal and scaleless embryos.

We have examined the immunolocalization of beta 1-integrin during feather development in the spino-lumbar tract of the backskin from normal and scaleless chick embryos. beta 1-integrin appears during early feather development in three distinct phases which correspond to important developmental events. The first phase (5-5 1/2 days of incubation; Hamburger and Hamilton [H.H.] stage 27) represents the period prior to the formation of dermis. During this phase, beta 1-integrin antiserum labels mesenchymal cells located in the central region of the spino-lumbar tract where the initiation site for feather development is located. The second phase (5 1/2-7 1/2 days of incubation; H.H. stages 28-32) corresponds to the period during which dermis is formed. The cells that make up the dermis are readily distinguished by their lack of beta 1-integrin immunostaining. The third phase (7 1/2-10 days of incubation; H.H. stages 33-36) begins with the sudden appearance of beta 1-integrin in the central and lateral regions of the dermis. The pattern of beta 1-integrin immunostaining in scaleless backskin becomes different from that of normal backskin during this phase. In normal backskin the dermal condensations of feather germs are not labeled with the beta 1-integrin antiserum. This produces a heterogeneous immunostaining pattern very similar to the pattern seen for Type I collagen (Mauger et al. [1982] Dev. Biol. 94:93-105). In contrast, homogeneous immunostaining is observed in the dermis of scaleless backskin. The initial time of appearance, manner of appearance, and pattern of integrin expression in the third phase suggest that beta 1-integrin may be involved in the stabilization of the feather pattern. We also observed the appearance of beta 1-integrin on the epidermal basal cells during the time of feather follicle formation. The beta 1-integrin antiserum reacts strongly with the baso-lateral surfaces of normal basal cells, yet the basal surfaces of the scaleless basal cells are unstained. This lack of immunostaining along the basal surfaces of the scaleless basal cells may relate to the abnormal adhesion between the epidermis and dermis in scaleless backskin.

Animals↗

PubMed1994

Mammographic appearances in Paget's disease of the breast.

The mammographic appearances in 17 patients with Paget's disease of the breast were retrospectively reviewed. Appearances were normal in only five cases. Microcalcification was present in 10 including one where it encircled the nipple. A radiologically-visible tumour was seen in nine breasts with carcinoma being diagnosed in a further three by the pattern of calcification alone. Multifocal tumours were present in four breasts but no lymphadenopathy or contralateral tumours were seen. Despite high quality radiography a tumour was only detected in 71% of cases of Paget's disease and radiographers should be trained to recognize the condition clinically. Patients without a visible tumour may be suitable for radiotherapy.

Adult↗

PubMed1993

Region-specific patterns of beta keratin expression during avian skin development.

The transient embryonic layers primarily composed of a periderm and subperiderm cover most regions of the chick embryo and are the first suprabasal cell layers covering the body ectoderm. This study presents evidence for regional variation in the expression of beta keratin in the embryonic layers. Here we show that the embryonic layers covering the anterior metatarsal region of the chicken hindlimb (scutate scale forming region) produce several members of the beta keratin family of polypeptides, designated beta (beta) 1-7. These specific polypeptides are later expressed in this region exclusively in the thick, cornified beta strata of mature scutate scales. In contrast to this sequence of events, the embryonic layers overlying the epidermis of the ventral foot pad (reticulate scale-forming region) and those covering the epidermis in apteric regions of the body produce beta keratin polypeptides beta 1-3 and beta 2,3, respectively, but no subsequent expression of these proteins occurs in the mature epidermises of these regions. Furthermore, we find that the embryonic layers of the skin overlying the anterior metatarsal region of birds homozygous for the mutation "scaleless" (sc/sc), which completely lack scutate scales, produce the same members of the beta keratin family, beta 1-7, as the embryonic layers and beta strata of normal scutate scales. Thus, the accumulation of specific beta keratin polypeptides in the developing anterior metatarsal region appears to occur in two distinct phases; first, an early region-specific expression in cells of the embryonic layers followed by a second phase of expression which occurs in conjunction with appendage morphogenesis.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMed1992

Avian scale development. XVII: The epidermis of the scaleless (sc/sc) anterior metatarsal skin is determined, but the dermis lacks permissive cues for the patterned expression of the determined state.

Embryos homozygous for the gene scaleless (sc/sc) completely lack scutate scales and the beta strata which characterize terminal differentiation of the scale ridges located on the anterior metatarsal region of the foot. Although the sc/sc epidermis cannot undergo scale morphogenesis, it can respond to the inductive dermal ridges of normal scutate scales by generating beta strata. Recently, we discovered that the anterior metatarsal epidermis of normal embryos becomes committed to the formation of beta strata prior to morphogenesis of definitive scale ridges. Here, we examined the possibility that the sc/sc anterior metatarsal epidermis also becomes determined, i.e., committed to scutate scale-specific terminal differentiation. Experimental tissue recombinants were used to assess the ability of the sc/sc epidermis to generate beta strata. The results show that the germinative cells of the 15-day sc/sc epidermis are committed to generating beta strata, even though they have not undergone scutate scale morphogenesis. Thus, the mechanisms involved in establishing epidermal determination must differ form those regulating scale morphogenesis. In addition, we examined the formation of patterned, permissive cues in the anterior metatarsal and footpad dermises of sc/sc embryos. Analysis of recombinants showed that both the 15- and 20-day dermises from the sc/sc anterior metatarsal region fail to provide cues for beta stratum formation, when associated with the determined 15-day scutate scale epidermis. Likewise, the 15-day sc/sc footpad dermis cannot support beta stratum formation. However, 20-day sc/sc footpad dermis is able to support the generation of a few abnormally patterned beta strata, demonstrating that sc/sc dermis which has experienced even limited morphogenesis is able to provide permissive cues for the terminal differentiation of the scutate scale epidermis.

Animals↗

PubMed1992

Avian scale development. XVI. Epidermal commitment to terminal differentiation is prior to definitive scale ridge formation.

Germinative cells of the scutate scale epidermis from 15-day embryos are committed to appendage-specific, beta stratum formation in association with a foreign dermis. Commitment precedes the time (17 days of development) at which beta strata are actually present in their site-specific locations along the outer surface of each scutate scale. This observation suggested the possibility that commitment to beta stratum formation might be occurring as the outer epidermal surface of each scutate scale first becomes established between 12 and 13 days of development. It is at this time that the scale epidermis loses its ability to participate in feather morphogenesis and cell proliferation becomes restricted to a true stratum basale. To examined the ability of the presumptive scutate scale epidermis to generate beta strata in the absence of the inductive scutate scale dermis, scutate scale epidermis from 11-, 12-, and 13-day embryos was recombined with 15-day reticulate scale dermis and grown for 7 or 9 days. The dermis of reticulate scales does not induce beta stratum formation, but it does support differentiation of a beta stratum by the determined 15-day scutate scale epidermis. Using immunohistological and biochemical analyses of beta-keratins, we find that each of these presumptive scutate scale epidermises is competent to generate appendage-specific beta strata in the absence of the scutate scale dermis. This determination is occurring prior to scale ridge morphogenesis and differentiation of the epidermis into the distinct outer and inner epidermal surfaces of the scale ridge. The restricted distribution of beta strata to the apical domes of individual reticulate-like scales demonstrates that the germinative cells of the committed epidermises are responding to patterned cues. This study also suggests that all basal cells of the presumptive scutate scale epidermis are initially endowed with the ability to generate cells that form a beta stratum.

Animals↗

PubMed1991

Expression of the cell adhesion molecules, L-CAM and N-CAM during avian scale development.

To examine the involvement of cell adhesion molecules in the inductive epithelial-mesenchymal interactions during avian scale development, a study of the spatiotemporal distribution of L-CAM and N-CAM was undertaken. During scutate scale development, L-CAM and N-CAM are expressed together in cells of the transient embryonic layers destined to be lost at hatching. The ongoing linkage of the cells of these layers by both CAMs sets them apart, early in development, as unique cell populations. L-CAM and N-CAM were also expressed simultaneously at the basal surface of the early germinative cells where signal transduction is presumed to occur. In spite of the differences in cell shape, adhesion, density and proliferative state between populations of epidermal placode and interplacode cells, the expression of L-CAM and N-CAM appeared to be uniform and nondiscriminating for these discrete cell lineages. The same pattern of L-CAM and N-CAM expression was observed during morphogenesis of reticulate scales that develop without placode formation. While L-CAM and N-CAM are present during the early stages of scale development and most likely function in cell adhesion, the data do not support a role for these adhesion molecules in the formation of the morphogenetically critical placode and interplacode cell populations. In both scale types, L-CAM became predominantly epithelial, and N-CAM became predominantly dermal as histogenesis occurred. Initially, N-CAM was concentrated near the basal lamina where it may be involved in the reciprocal epidermal-dermal interactions required for morphogenesis. However, as development of the scales progressed, N-CAM disappeared from the tissues. L-CAM expression continued in the epidermis and was intense on all suprabasal cells undergoing differentiation into either an alpha-stratum or beta-stratum. However, L-CAM was more prevalent on the basal cells of alpha-keratinizing regions than on the basal cells of beta-keratinizing regions.

Animals↗

PubMed1991

Region-specific expression of scutate scale type beta keratins in the developing chick beak.

This study shows that different patterns of scutate scale type beta keratins are accumulated in the three adjacent structures of the embryonic chick beak: periderm, egg tooth, and cornified beak. The cornified beak accumulates all of the beta keratins of scutate scale except pp2,3. The periderm, which is the outermost, multilayered covering of the whole embryonic beak, accumulates only beta keratins 2,3, and p2,3 of the scutate scale pattern. The egg tooth, which is the rounded elevation on the dorsal surface of the upper beak, and the embryonic claw accumulate greatly reduced levels of 2,3 and p2,3 compared to scutate scale. Like cornified beak, the claw does not accumulate pp2,3, but both tissues express a potentially new beta keratin, beta keratin 8. Neither the histidine rich "fast" proteins (HRPs), which are expressed in embryonic scutate scales and feathers, nor the avian cytokeratin associated proteins (cap-1 and cap-2), which are expressed in scutate and reticulate scales, are expressed in any of the embryonic beak structures or in the claw. The implications of these findings with regard to regulation of terminal differentiation of avian skin are discussed.

Animals↗

PubMed1991

Biochemical identification and immunological localization of two non-keratin polypeptides associated with the terminal differentiation of avian scale epidermis.

The expression of two previously uncharacterized polypeptides produced in epidermal cells of chick reticulate and scutate scales during late embryonic scale histogenesis and in hatchling birds has been studied biochemically and immunologically. These polypeptides have been identified by two-dimensional pH gradient gel electrophoresis as basic in charge, with apparent molecular weights of 20 and 23 kD, and they have been characterized immunologically and by amino acid analysis as non-keratin in nature. Monoclonal antibodies which react with both polypeptides have been used for immunohistochemical and immunogold electron-microscopic localization. Immunoreactivity was observed in suprabasal cells of reticulate scale epidermis, where it codistributed with bundles of alpha-type cytokeratins in the alpha-keratin-rich layers of epidermis known as the alpha stratum and in suprabasal cells of the outer epidermal surface of scutate scales, where it codistributed with alpha- and beta-type keratin filament bundles in the beta-keratin-rich layers of epidermis known as the beta stratum.

Aging↗

PubMed1991

Avian scale development. XIII. Epidermal germinative cells are committed to appendage-specific differentiation and respond to patterned cues in the dermis.

The ability of the germinative cell population of scutate scale epidermis to continue to generate cells that undergo their appendage-specific differentiation (beta stratum formation), when associated with foreign dermis, was examined. Tissue recombination experiments were carried out which placed anterior metatarsal epidermis (scutate scale forming region) from normal 15-day chick embryos with either the anterior metatarsal dermis from 15-day scaleless (sc/sc) embryos or the dermis from the metatarsal footpad (reticulate scale forming region) of 15-day normal embryos. Neither of these dermal tissues are able to induce beta stratum formation in the simple ectodermal epithelium of the chorion, however, the footpad dermis develops an appendage-specific pattern during morphogenesis of the reticulate scales, while the sc/sc dermis does not. Morphological and immunohistological criteria were used to assess appendage-specific epidermal differentiation in these recombinants. The results show that the germinative cell population of the 15-day scutate scale epidermis is committed to generating suprabasal cells that follow their appendage-specific pathways of histogenesis and terminal differentiation. Of significance is the observation that the expression of this determined state occurred only when the epidermis differentiated in association with the footpad dermis, not when it was associated with the sc/sc dermis. The consistent positioning of the newly generated beta strata to the apical regions of individual reticulate-like appendages demonstrates that the dermal cues necessary for terminal epidermal differentiation are present in a reticulate scale pattern. The observation that beta stratum formation is completely missing in the determined scutate scale epidermis when associated with the sc/sc dermis adds to our understanding of the sc/sc defect. The present data support the conclusion of earlier studies that the anterior metatarsal dermis from 15-day sc/sc embryos lacks the ability to induce beta stratum formation in a foreign epithelium. In addition, these observations evoke the hypothesis that the sc/sc dermis either lacks the cues (generated during scutate and reticulate scale morphogenesis) necessary for terminal differentiation of the determined scutate scale epidermis or inhibits the generation of a beta stratum.

Animals↗

PubMed1991

The initial expression and patterned appearance of tenascin in scutate scales is absent from the dermis of the scaleless (sc/sc) chicken.

Morphogenesis of the anterior metatarsal skin (scutate scale region), from 9.5 to 12 days of development, results in the formation of orderly patterned scale ridges. It is after the initial formation of the Definitive Scale Ridge that the characteristic outer and inner epidermal surfaces differentiate. The hard, plate-like beta stratum, with its unique beta keratins, characterizes the epidermis of the outer surface, while the epidermis of the inner surface elaborates an alpha stratum. The anterior metatarsal region of the scaleless mutant does not undergo scale morphogenesis. Therefore, scale ridges do not form nor do the outer and inner epidermal surfaces with their characteristic beta and alpha strata. We have found that the extracellular matrix molecule, tenascin, first appears in the scutate scale dermis at 12 days of development when the scale ridge is established. Tenascin is found in the dermis only under the scale ridge and is not associated with the dermal-epidermal junction. Tenascin is not found in scaleless anterior metatarsal dermis at this time. As outgrowth of the Definitive Scale Ridge takes place, tenascin distribution correlates closely with the formation of the outer epidermal surface of each scale ridge. By 16 days of development tenascin is also found in close association with the dermal-epidermal junction. Tenascin does not appear in scaleless anterior metatarsal dermis until 16 days of development and then it is randomly and sparsely distributed at the dermal-epidermal junction. Tenascin's initial appearance and pattern of distribution in the scutate scale dermis and its abnormal expression in the scaleless dermis suggest that morphogenesis plays a significant role in regulation of its expression.

Animals↗

PubMed1990

Beta-keratin expression in avian tongue cell aggregates.

Rotation-mediated aggregation was used to test the capacity of early embryonic tongue cells from different regions of the tongue to reorganize histotypically and terminally differentiate in vitro. Cells dissociated from the anterior ventral (AV) or posterior ventral (PV) surface of the 12 day embryonic tongue were cultured 6 days, fixed, and embedded in paraffin. Indirect immunofluorescence microscopy was used to demonstrate the occurrence and distribution of beta-keratins, the presence of which represented regional and differentiation specific markers of late tongue development. Aggregates of AV cells were organized into a beta-keratin-producing stratified epithelium. Similar PV aggregates formed only an alpha-keratin-producing stratified epithelium. The epithelial cells of these tongue tissues constructs expressed alpha- and beta-keratins in a manner consistent with the temporal and histotypical expression of keratins observed in vivo.

Animals↗

PubMed1989

Immunocytochemical localization and biochemical analysis of alpha and beta keratins in the avian lingual epithelium.

The alpha and beta keratins are found as 10-nm and 3-nm cytoplasmic filaments, respectively. While the alpha keratins are produced in essentially all vertebrate epithelia (Franke et al.: Exp. Cell Res., 116:429-445, 1978; Sun et al.: Proc. Natl. Acad. Sci. USA, 76:2813-2817, 1979), the beta keratins have been demonstrated only in specific epithelial tissues of birds and reptiles (Sawyer et al.: In: Biology of the Integument: Vertebrates. J. Bereiter-Hahn, A.G. Matoltsy, and K.S. Richards, eds. Springer-Verlag, Berlin, Vol. 2, pp. 194-238, 1986; Landmann: In: Biology of the Integument: Vertebrates. J. Bereiter-Hahn, A.G. Matoltsy, and K.S. Richards, eds. Springer-Verlag, Berlin, Vol. 2, pp. 150-187, 1986). Recently, Homberger and Brush (Zoomorphology, 106:103-114, 1986) have demonstrated that within the lingual epithelium of parrots, beta keratins are expressed exclusively in the anterior ventral region. While it is well established that epidermal-dermal interactions are important for the regional expression of the beta keratin genes in the avian scutate scales and feathers, little is known about the expression of beta keratins in other epithelial structures such as the tongue. We have used biochemical and immunocytochemical techniques to analyze the alpha and beta keratins of the lingual epithelium of the chick as an initial step in the characterization of this model system for developmental studies. We have found that alpha keratins are present throughout the lingual epithelium. The anterior ventral epithelium contains alpha keratin polypeptides characteristic of skin-type differentiation, while the epithelium of the dorsal and posterior ventral regions contains alpha keratin polypeptides characteristic of esophageal-type differentiation (O'Guin et al.: In: Current Topics in Developmental Biology: The Molecular and Developmental Biology of Keratins. A.A. Moscona and A. Monroy, eds. R.H. Sawyer, vol. ed. Academic Press, New York, Vol. 22, pp. 282-306, 1987). Beta keratins are produced only in the differentiated epithelial cells of the anterior ventral region of the tongue. Immunoelectron microscopy demonstrates that the alpha and beta keratins of the stratum intermedium and corneum of the anterior ventral region are found together in the large filament bundles characteristic of this region. The preexistence of the alpha keratins in the cells destined to produce beta keratins as well as the colocalization of these keratins in the filament bundles of these cells suggests that a functional relationship may exist between the alpha and beta keratins.

Animals↗

PubMed1989

Keratinization of the outer surface of the avian scutate scale: interrelationship of alpha and beta keratin filaments in a cornifying tissue.

The outer surface of adult Gallus domesticus scutate scale was studied as a model for epidermal cornification involving accumulation of both alpha and beta keratins. Electron-microscopic analysis demonstrated that the basal cells of the adult epidermis contained abundant lipid droplets and that filament bundles and desmosomes were distributed throughout the cell layers. Indirect immunofluorescence microscopy and double-labeling immunogold-electron microscopy confirmed that the stratum germinativum contained alpha keratin but not beta keratin. Beta keratins were first detected in the stratum intermedium and were always found intermingled with filament bundles of alpha keratin. As the differentiating cells moved into the outer regions of the stratum intermedium and the stratum corneum, the large mixed keratin filament bundles labeled increasingly more with beta keratin antiserum and relatively less so with alpha keratin antiserum. Sodium dodecyl sulfate-polyacrylamide gel analysis of vertical layers of the outer surface of the scutate scale confirmed that cells having reached the outermost layers of stratum corneum had preferentially lost alpha keratin. The mixed bundles of alpha and beta keratin filaments were closely associated with desmosomes in the lower stratum intermedium and with electron-dense aggregates in the cytoplasm of cells in the outer stratum intermedium. Using anti-desmosomal serum it was shown that these cytoplasmic plaques were desmosomes.

Animals↗

PubMed1988

Avian scale development: XI. Immunoelectron microscopic localization of alpha and beta keratins in the scutate scale.

Epithelial-mesenchymal interactions play important roles in morphogenesis, histogenesis, and keratinization of the vertebrate integument. In the anterior metatarsal region of the chicken, morphogenesis results in the formation of distinct overlapping scutate scales. Recent studies have shown that the dermis of scutate scales is involved in the expression of the beta keratin gene products, which characterize terminal differentiation of the epidermis on the outer scale surface (Sawyer et al.: Dev. Biol. 101:8-18, '84; Shames and Sawyer: Dev. Biol. 116:15-22, '86; Shames and Sawyer: In A.A. Moscona and A. Monroy (eds), R.H. Sawyer (Vol. ed): Current Topics in Developmental Biology. Vol. 22: The Molecular and Developmental Biology of Keratins. New York: Academic Press, pp. 235-253, '87). Since alpha and beta keratins are both found in the scutate scale and are members of two different multigene families, it is important to know the precise location of these distinct keratins within the epidermis. In the present study, we have used protein A-gold immunoelectron microscopy with antisera made against avian alpha and beta keratins to specifically localize these keratins during development of the scutate scale to better understand the relationship between dermal cues and terminal differentiation. We find that the bundles of 3-nm filaments, characteristic of tissues known to produce beta keratins, react specifically with antiserum which recognizes beta keratin polypeptides and are found in the embryonic subperiderm that covers the entire scutate scale and in the stratum intermedium and stratum corneum making up the platelike beta stratum of the outer scale surface. Secondly, we find that 8-10-nm tonofilaments react specifically with antiserum that recognizes alpha keratin polypeptides and are located in the germinative basal cells and the lowermost cells of the stratum intermedium of the outer scale surface, as well as in the embryonic alpha stratum, which is lost from the outer surface of the scale at hatching. The alpha keratins are found throughout the epidermis of the inner surface of the scale and the hinge region. Thus, the present study further supports the hypothesis that the tissue interactions responsible for the formation of the beta stratum of scutate scales do not directly activate the synthesis of beta keratins in the germinative cells but influence these cells so that they or their progeny will activate specific beta keratin genes at the appropriate time and place.

Animals↗