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Biomedical subjects

R Hackler

Publications and source records attributed to R Hackler.

28 records · Page 2Linked to original sources
PubMed1990

Transferrin subtypes in Iran.

The frequency of transferrin Tf C subtypes has been determined by double one-dimensional electrophoresis of plasma samples from Moslems (n = 91), Zoroastrians (n = 97), Jews (n = 88) and Armenians (n = 88) of Iran. The Zoroastrians show the lowest frequency of TfC1 (0.4999) and highest frequencies of TfC2 and TfC3 (.02215, and 0.2783, respectively). The Jews have the highest TfC1- and the lowest TfC2- and TfC3 frequencies (0.8011, 0.1478, and 0.0512, respectively). It could be shown that the differences between Zoroastrians and Jews are highly significant (p less than 0.001). Arbitrary subtyping of transferrin Tf B and TfD phenotypes could be done on samples from three regional groups of Iran: North: n = 282, Central: n = 548, and South: n = 587 into Tf B (Iran 1, 2, 3 and 4) and Tf D (Iran 1, 2 and 3) was performed according to mobilities relative to the transferrin C protein during polyacrylamide gel electrophoresis and by relative pI deviations from the Fe2-transferrin C1 protein after isoelectric focussing. The allele frequencies found in the total sample (n = 1417) are: TfB1 = 0.0003, TfB2 = 0.0010, TfB3 = 0.0042, TfB4 = 0.0007; TfD1 = 0.0017, TfD2 = 0.0014, and TfD3 = 0.0010.

Alleles↗
PubMed1988

Flattening and/or expanding of pH gradients in isoelectric focusing gels exemplified with PhastSystem.

A simple method of flattening and/or expanding of pH gradients in isoelectric focusing is described for any pH interval desired: to modify pH gradients near one electrode a paper strip soaked with carrier ampholytes is applied onto the gel close to the opposite electrode. In order to flatten central parts of pH intervals paper strips are applied onto the gel at both electrodes. Conditions and criteria (e.g. amount and pH intervals of carrier ampholytes, width and localization of the paper strip, separation period) for optimization are presented with PhastSystem using ready-made gels with three different pH intervals and pI marker proteins (Pharmacia). Examples utilizing erythrocyte lysates are presented.

Ampholyte Mixtures↗
PubMed1987

Mentor inflatable penile prosthesis.

Technical complications requiring frequent surgical revision of the most commonly used penile component prosthesis have led to the development of a new inflatable device that has been implanted in 202 patients. This hydraulic device provides excellent functional qualities; no connector separations, aneurysms, or ruptures have been encountered in these patients. Reoperations became necessary in 8 per cent of these patients. Cosmetic and functional results are good, and patient acceptance has been excellent.

Erectile Dysfunction↗
PubMed1986

Experience with the Mentor inflatable penile prosthesis.

We present our experience with the new Mentor inflatable penile prosthesis for the treatment of organic impotence. Technical complications requiring frequent revision of the most commonly used inflatable prosthesis have led to the development of a new device, which we have used in 116 patients. This hydraulic device provides excellent functional qualities. No connector separations, aneurysms or ruptures have been encountered. Reoperations became necessary in 10.5 per cent of the patients. Cosmetic and functional results were good, and patient acceptance has been excellent.

Erectile Dysfunction↗
PubMed1980

Double one-dimensional electrophoresis of human serum transferrin: a new high-resolution screening method for genetically determined variation.

With one-dimensional electrophoresis or electrofocusing the selective demonstration of proteins and their genetically determined variants from complex compositions like the physiological body fluids often is possible only with specific staining techniques or immunofixation. By the method described here samples are separated by PAG electrophoresis and a selected detail of the electropherogram is further resolved by PAG electrofocusing. The resulting pattern of bands after a usual protein stain can easily be interpreted due to the reduction of the number of bands. Informative bands hidden in a one-dimensional pherogram by overlapping with one or more other bands can be selectively visualized by the one-dimensional combination of two electrophoretic methods. With the double one-dimensional technique several hundred samples can be analyzed per person day. Thus the application in electrophoretic screening studies appears to be of special interest. The method is demonstrated by using the polymorphism of human serum transferrin as an example. A new variant of Tf-C with a high incidence in the German population is demonstrated.

Adult↗
PubMedDate not supplied

Carbohydrate-deficient transferrin is not affected by serum separators.

We studied the possible effects on serum carbohydrate-deficient transferrin (CDT) determination by a CDTect (Pharmacia) method of serum isolation in four different types of blood-collection tubes, namely: (1) glass tubes (glass Vacutainer tubes with no additive); (2) S-Monovette Neutral tubes (plastic tubes with no additive); (3) S-Monovette Serum tubes (plastic tubes with kaolin-coated plastic granulate coagulation accelerator); and (4) S-Monovette Serum/Gel tubes (plastic tubes with kaolin-coated plastic granulate and a polymerized acrylamide resin). Using Passing and Bablok regression analysis, we did not observe significant differences in CDT concentrations determined in 58 serum samples using any of these four blood-collection systems.

Biological Factors↗
PubMedDate not supplied

CDTect-RIA and CDTect-EIA for determination of serum carbohydrate-deficient transferrin compared.

CDTect-RIA and CDTect-EIA for determination of serum carbohydrate-deficient transferrin (CDT) by radioimmunoassay and enzyme immunoassay respectively were tested for equality and precision in four European laboratories. For correlational studies, serum samples with CDT concentrations up to 130 U/l were analysed in accordance with a uniform trial schedule. The regression of CDT values obtained by the two procedures was computed for each laboratory using the method of Passing and Bablok. Slopes and intercepts of the regression functions did not differ significantly from the values 1 or 0, as proved by the corresponding 95% confidence intervals. Precision studies were computed using analysis of variance. For CDT concentrations at the upper reference limit for men, the within-day coefficients of variation (CVs) ranged between 0.7 and 6.4% (median 5.2%) for CDTect-RIA and from 4.3 to 9.2% (median 6.2%) for CDTect-EIA. The corresponding pure between-day CVs were 5.0-18.5% (median 9.8%) and 3.5-14.5% (median 10.9%). The study demonstrates the equality of CDT values obtained by CDTect-RIA and CDTect-EIA. According to this study, the two methods can be used interchangeably without getting fluctuating CDT values, e.g. in longitudinal studies.

Alcoholism↗
PubMedDate not supplied

Age-related alterations of the blood-brain-barrier (bbb) permeability to protein molecules of different size.

Transfer processes of differently sized protein molecules across the blood-brain-barrier (bbb) were studied in "normal" , respectively, diseased elderly humans. Lumbar CSF/serum ratio of albumin contents increased significantly with age in a control group, but it was diminished in patients suffering from inflammatory or non-inflammatory diseases. Ratio of alpha 2-macroglobulin contents increased with age, especially in the diseased elderly. Concentration ratios of lysozyme, orosomucoid, lactoferrin, IgG, IgA and IgM decreased with age in a control group and especially decreased in a patient group. The data are discussed with respect to two different transfer processes which appeared to be altered in age and/or disease processes.

Adolescent↗
PubMedDate not supplied

Transport and production of cerebrospinal fluid (CSF) change in aging humans under normal and diseased conditions.

In three control groups of lumbar (L), cisternal (C), and ventricular (V) cerebrospinal fluid (CSF) with prealbumin contents of ventricular origin (CSF-V-prealbumin) within the reference range, indications for an increased transport of V-CSF into spinal space are presented in the elderly as well as an elevated production of V-CSF. Indications for a diminished V-CSF production, respectively, an increased transport of CSF-V-prealbumin into the spinal space were found in elderly patients suffering from diverse CNS diseases. As these alterations proved to be not disease-specific, the age-related increase of CSF transport was discussed with respect to brain atrophy and enlarged CSF spaces in the elderly central nervous system (CNS). CSF serum prealbumin content, an indicator of blood-brain-barrier function, increased with age similarly to albumin in the "normal" elderly, respectively with diverse CNS diseases. Age- and disease-related decreases of albumin and prealbumin contents in blood serum may lead to wrong interpretations.

Adolescent↗