[The germ content in turbine cooling water and its reduction in a dental outpatient polyclinic].
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Biomedical subjects
Publications and source records attributed to R Hergt.
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Noise levels were measured in the Gynaecological Hospital of the University of Rostock. Patients, medical doctors, and nurses were interviewed on their own molestation. The equivalent permanent sound level, Leq, was 63.5 dB (AI) on average in patient rooms on daytime, while the average night value was 59.8 dB (AI). Maximum sound pressures, Lmax, varied between 55 and 80 dB (AI) in patient rooms. An Lmax of 85 dB (AI) was measured on a ward corridor, when instruments were cleaned in the wash-basin of a service room. Molestation by outdoor noise was reported by 88 per cent of 85 patients interviewed, while 29 per cent felt disturbed by indoor noise. The noise was perceived as disturbing also by the personnel. While the gravest role for the hospital investigated by played by outdoor noise, greatest attention should be given also to the control of indoor noise.
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It is reported on four own observations of atypical mycobacteriosis, which had been evident within the clinical examinations of a larger mixed number of patients with internal diseases. In three patients pathogenic significance must be ascribed to the proved atypical mycobacteria. All the three had other basic diseases. Only in one patient it appeared as a purely pulmonary form of manifestation. On the basis of a further observation is shown that a single proof of atypical mycobacteria may be regarded only as accidental findings. The clinical symptomatology of this group of diseases is multiform. The differential-diagnostic considerations are usually determined by the basic disease. As apart from this the proof of atypical mycobacteria is difficult, infections with these microorganisms might at present not infrequently remain infrequently. The observation of a disease by mycobacterium xenopi emphasizes that also in this microorganism good chances of success may be given by medicamentous antituberculotic therapy.
In cooperation of eight laboratories the new surface-active substance Ditalan WO hc was compared with Präwozell NBX (Nekal BX) used for homogenization and decontamination of the specimens for culture investigations of mycobacteria in routine. Under routine conditions Ditalan showed a slight, but statistically not significant advantage. Out of 1672 specimens pretreated with both detergents 482 cultures were positive in all, 429 (89,0%) after homogenization with Ditalan and 413 (85,7%) after pretreatment with Präwozell. The rate of contamination of the cultures was low in both cases (about 0,5%). Ditalan had a better mucolytic effect than Präwozell. In quantitative investigations the number of positive cultures was equal with both methods, but the number of colonies was somewhat higher and the time of growth was a little shorter after using Ditalan. In the third part the influence of both detergent-reagent-solutions on mycobacteria of different species was compared to that of physiological sodium chloride solution. The rate of growth was decreased by both the detergents in nearly the same degree. But the damaging effect is caused less by the detergent itself than by the sodium hydroxide contained in the solution used for pretreatment of the specimens.
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Sera of patients, blood donors and cattle sera were examined by means of diffusion--in--gel technique using self produced antigens, prepared from H 37 Ra, one fresh isolated strain of M. tbc and BCG Jena. 38.2% of 421 patients with pulmonary tuberculosis reacted positive. Positive reactions were observed in 55.7% of 199 patients suffering from another infectious disease and in 31;7% of normal sera. Most precipitation lines were found with the antigen prepared from H37 Ra after incubation for 7 months. In human sera as well as in cattle a dependence of reactions upon mode of antigen production was evident.
Sera of patients with pulmonary and extrapulmonary tuberculosis, of blood donors and cattle sera were researched in hemagglutination reaction, using untreated and papain-treated sheep erythrocytes, and in hemolysis reaction. 58% of patients with pulmonary tuberculosis and 23% of normal sera, reacted positive in at least one of the 3 reactions. 24 sera of 44 patients with extrapulmonary tuberculosis were found to be positive. The titer is important for diagnosis. In normal sera the highest titers were 1:64 in all reactions. Titers of 1:128 are to be considered as proof of tuberculosis. Considering the height of titers the hemagglutination-hemolysis reaction can also be used for diagnosis of tuberculosis in cattle.
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Agglutination with serotype patoc (Patoc 1) was found in 72 of 125 cases of leptospirosis. Patoc-agglutination occured frequently in leptospirosis icterohaemorrhagiae, less frequently in leptospirosis grippotyphosa and rarely in leptospirosis tarassovi. 53 cases of leptospirosis were not diagnosed when using Patoc 1 as single antigen. So we cannot recommend to use serotype patoc as the sole antigen for microscopic agglutination test. We observed 1 case in which the reaction with Patoc 1 happened earlier than the reaction with the pathogen serotypes. For this reason the battery of antigens for carrying out microscopic agglutination test should include serotype patoc, too.
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