Biomedical subjects

R Honig

Publications and source records attributed to R Honig.

5 recordsLinked to original sources

PubMed1977

Somatic cell hybrids between human lymphoma lines. III. Surface markers.

Hybrids between two human lymphoma lines, Raji and Daudi (8A) and Raji BJAB (83) were examined for genetically determined and/or differentiation-related surface markers. HL-A B ce-l alloantigens, Fc and complement receptors, EBV receptors and beta2 microglobulin showed an autonomous ("co-dominant") expression in the hybrid. This is in contrast to most previous studies on other differentiation markers, involving as a rule crosses between cells of different lineages, where the differentiated pattern usually became "eclipsed" in the hybrid. Staining of activated complement and complement consumption tests showed intermediate or partially suppressed expression in the hybrids. This may be viewed in relation to the fact that these reactions do not merely depend on complement binding to the receptor, but also on subsequent activation and binding of the activated complement. A more complex interaction is also suggested for immunoglobulin production. Surface immunoglobulin showed a suppressive or intermediate pattern in both hybrids, whereas intracellular kappa chain production showed an amplification in the 83 hybrid. The beta2 microglobulin deficiency of the Daudi parent was corrected in the Raji/Daudi hybrid. Two new HL-A specificities,A10 and BW17, appeared on this hybrid which were not present on the parental lines. This suggests that the HL-A deficiency of the Daudi cell is due to its lack of beta2 microglobulin.

B-Lymphocytes↗

PubMed1976

Inducibility of the Epstein-Barr virus (EBV) cycle and surface marker properties of EBV-negative lymphoma lines and their in vitro EBV-converted sublines.

Two EBV-negative lymphoma lines of human B-cell origin, BJAB and Ramos, were compared with altogether six of their in vitro EBV-converted, EBNA- and EBV-DNA-carrying sublines (four of Ramos and two of BJAB derivation). All converted lines closely resembled the parental line with regard to karyotype and HL-A and B antigen typing. Induction of EBV antigens (EA and VCA) by P3HR-1 virus superinfection was either similar in the converted and the negative lines, or somewhat increased in certain converted lines. These findings argue against a simple, virally determined repressor model and emphasize the role or cellular controls in restricting the EBV cycle in virus-carrying B-lymphocyte lines of human origin. IUdR inducibility varied in the different converted lines. There was a possible relationship between average number of EBV-genome equivalents per cell and inducibility. Converted sublines did not differ from the original negative lines with regard to surface immunoglobulin and Fc receptors. There was a dramatic increase in complement-consuming ability, however, following EBV conversion. Among the EBV-positive lines, there was a linear relationship between complement-consuming and EBV-receptor activity, the latter measured by a quantitative absorption test.

Antigens, Viral↗

PubMed1976

The absence of B-cell antigen B2 from leukaemia cells and lymphoblastoid cell lines.

60 leukaemia patients with acute mylogenous leukaemia, chronic myelocytic leukaemia, acute lymphatic leukaemia, and chronic lymphocytic leukaemia were tested for five specificities of the new B-lymphocyte alloantigenic system. Two specificities of the first B-cell locus were significantly lower in frequency than in B lymphocytes from 105 controls. B-cell group 1 was found in 18% of leukaemia patients vs. 48% of controls (p less than 0.025) and B-cell group 2 was found in 0% of leukaemia patients vs 23% of controls (p less than 0.025). Group 2 was also absent in 37 cultured lymphoblastoid lines. It is suggested that resistance to B-cell leukaemia and to Epstein-Barr virus transformation in vitro may be linked to a B-lymphocyte specificity on the first B-lymphocyte locus.

Adult↗

PubMed1976

B lymphocyte alloantigen specificities present on cultured lymphoblastoid cell lines.

Thirty-three of 34 cultured human lymphoblastoid B cell lines have been shown to express four out of five polymorphic non-HLA specificities expressed by normal B lymphocytes. The specificities were detected by 32 alloantisera produced by absorption with pooled platelets to remove HLA activity and selected from over 400 pregnancy sera. One B group (B2) which was expressed by 23% of a panel of normal B lymphocytes was not found on any of the cultured lines tested. Four T cell lines tested and myeloid line (K562) did not react with any of the B cell alloantisera.

B-Lymphocytes↗

PubMed1976

Blocking of HLA and B lymphocyte alloantigens with F (ab')2 fragments of rabbit antibodies.

F(ab')2 fragments of rabbit antibodies specific for human B lymphocytes will block human anti-B lymphocyte alloantibodies. F(ab')2 of anti-beta2m will block HLA alloantibodies. The F(ab')2 fragments can be used to improve the typing of B cell lines and certain leukemia cells which express both HLA and B lymphocyte alloantigens. Pretreatment of these cells with anti-beta2m F(ab')2 preparations allows the observation of B cell alloantigen reactions independently of HLA reactions. Similar use of rabbit anti-B cell F (ab')2 allows HLA reactions to be observed free from the "extra reactions" caused by B alloantibodies that are present in many HLA typing sera.

Animals↗