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Biomedical subjects

R I Cue

Publications and source records attributed to R I Cue.

16 recordsLinked to original sources

Implications of day-to-day variability on measurements of usual food and nutrient intakes.

Day-to-day variability in dietary intake makes it difficult to measure accurately the "usual" intake of foods and nutrients. The objectives of the present study were to estimate within- and between-subject variability for foods and nutrients by adjusted and unadjusted models and to assess the number of days required to assess nutrient and food group intakes accurately by two different methods. Adult men and women aged 18-65 y (n = 1543) in the Food Habits of Canadians Study provided a 24-h recall. A repeat interview was conducted in a subsample to estimate components of variability. Within- and between-subject variability were determined by mixed model procedure (crude and adjusted for age, gender, education, smoking, family size and season). The number of days required to obtain various degrees of accuracy was ascertained by two methods, one that uses the variance ratio for groups and one that considers within-subject variability alone for individuals. Variance ratios were higher using the adjusted compared with the unadjusted method (e.g., for men, energy 1.07 vs. 0.49). More days were required to reflect usual intake with accuracy using the adjusted model (energy 5 vs. 2 d), indicating the need to control for confounders to obtain reliable estimates of intakes.

Adult↗

Impact of type traits on functional herd life of Quebec Holsteins assessed by survival analysis.

Survival analysis was used to study the effects of composite and descriptive linear type traits on functional herd life of Quebec Holsteins. Functional herd life was defined as the length of life from first calving to death, culling, or censoring, and was adjusted for 305-d milk production. The dataset contained information from 331,105 cows from Quebec province calving for the first time between 1981 and 1995; 58% of the records had type information. Weibull models were fitted to analyze the data. The hazard function was described as the product of a baseline hazard function and the time-independent effects of age at first calving and type, and the time-dependent effects of year of calving, stage of lactation x lactation number, annual change in herd size, 305-d milk production, and herd-year (random). Analyses were done one at a time for each type trait. The strongest relationships between survival and composite type traits were found for final score, mammary system, and feet and legs. Among the linear type traits, the highest impact on functional herd life was found for traits related to the udder.

Animals↗

Effects of insulin, recombinant bovine somatotropin, and their interaction on insulin-like growth factor-I secretion and milk protein production in dairy cows.

This trial was designed to test the effects of insulin, recombinant bovine somatotropin (rbST), and their interaction on milk protein and selected blood parameters in dairy cows. Eight Holstein cows (86 +/- 10 d in milk) were divided in two groups and used in two replicates of a Latin square design with four animals, four periods, and four treatments: 1) intravenous infusion of saline, 2) infusion of saline and subcutaneous administration of 40 mg of rbST per day, 3) intravenous infusion of 12 mg of insulin per day coupled with glucose infusion, and 4) rbST administration combined with insulin and glucose infusion. The glucose infusion rate was adjusted to maintain euglycemia. Each experimental period lasted 14 d: treatments were administered during the first 6 d, and no treatment was administered during the following 8-d resting phase. The average daily amount of glucose infusion needed to avoid hypoglycemia was 2.8 kg/cow when only insulin was infused as opposed to 2.2 kg/cow when both insulin and rbST were administered, indicating that either rbST causes a peripheral resistance to insulin or rbST increased liver gluconeogenesis or both. Data from the last 3 d of infusion were analyzed by using the SAS system for mixed models. Percent protein of milk tended to be lower (2.84 vs. 2.79%) and milk urea content was lower (16.6 vs. 14.8 mg/dl) during rbST administration, regardless of insulin infusion. Insulin infusion increased percent protein (2.78 vs. 2.85%) and percent casein (2.36 vs. 2.46%) and decreased milk urea content (17.1 vs. 14.3 mg/dl) regardless of rbST administration. For milk yield, protein yield, casein yield, lactose percent, and lactose yield, there were significant interactions between insulin and rbST administration. For example, casein yield averaged 1.17, 1.12, 1.20, and 1.28 kg/d for saline, insulin, rbST, and insulin combined with rbST, respectively. Similarly, there was a significant interaction between insulin and rbST on IGF-I levels, which were 122.5, 181.3, 342.3, and 492.2 ng/ml for saline, insulin, rbST, and insulin combined with rbST, respectively. In conclusion, these results clearly demonstrated that insulin interacts with bST in early lactation to improve milk protein synthesis and yield in dairy cows. These effects are probably mediated through a combination of bST nutrient mobilization, bST-induced gluconeogenesis, bST-induced insulin peripheral resistance, and bST/insulin synergism on insulin-like growth factor-I secretion and on mammary epithelial tissue.

Animal Nutritional Physiological Phenomena↗

Genetic analysis of herd life in Quebec Holsteins using Weibull models.

Survival analysis methodologies were used to study herd life in Canadian Holstein cows. Herd life was defined as true herd life or the length of time between first calving and censoring. True herd life adjusted for 305-d milk production was defined as functional herd life. Lifetime record (censored or completed) were from 331,147 Holstein cows registered in the Programme d'Analyse des Troupeaux Laitiers du Québec (PATLQ) that calved for the first time between March 1, 1981 and March 31, 1995. The Weibull (proportional hazards) model used to analyze true herd life and functional herd life contained a Weibull baseline hazard function and the time-dependent effects of year of first calving, lactation number by stage of lactation, annual change in herd size and herd-year (random), and the time-independent effects of the milk recording option (supervised or not) and age at first calving. The model for functional herd life included also the time-dependent effect of herd-year-parity class of 305-d milk production. Genetic differences between sires with regard to the hazard function of their daughters was clearly demonstrated. The hazard rate followed a different pattern in later lactations, particularly in the first 240 d in milk. Older age at first calving was found to be associated with higher risks of culling. Changes in herd size had a small impact on the hazard function of animals. The hazard decreased as production of the cow increased. Heritability in the log scale was 0.09 for true herd life and 0.08 for functional herd life, but when heritability was expressed on the original scale, the estimates for the two traits were 0.19 and 0.15, respectively. The difference in the median survival between a bull with an estimated transmitting ability of 0.6 and another bull with an estimated transmitting ability of 1.3 was 690 d or 1.7 lactations. Rank correlations between the official estimated transmitting abilities for true herd life and functional herd life and those obtained in this study were 0.62 and 0.66, respectively.

Aging↗

Correlations between first lactation and lifetime performance traits of Canadian Holsteins.

Data on 82,835 Holstein cows, daughters of 703 sires and with first calvings from 1979 to 1984 in 2384 herds enrolled in Quebec Dairy Herd Analysis Service, were analyzed for relationships of first lactation traits with traits for partial and total lifetime performance using multitrait REML methodology and a linear mixed model. Effects of herd-year-season, age at first calving, and proven sires were considered to be fixed, and effects of young sires and residuals were considered to be random. All known additive genetic relationships among sires were accounted for in construction of the relationship matrix. Individual lactation records were precorrected for the fixed effect of year and month of calving before lifetime totals were calculated. Each cow was given at least a 5-yr opportunity for production. All genetic and phenotypic correlations were positive except correlations of first lactation percentage traits. Correlations with partial lifetime performance were highest. Genetically, first lactation milk yield was highly correlated with most measures of lifetime performance, .64 to .92; correlations with measures of longevity were relatively smaller. Given high positive genetic correlations, selection singly on first lactation milk yield will improve all measures of lifetime performance; however, some measure of longevity should be considered in selection programs of dairy cattle.

Animals↗

Multitrait restricted maximum likelihood estimates of genetic and phenotypic parameters of lifetime performance traits for Canadian Holsteins.

Data on 82,835 Holstein cows, daughters of 703 sires and with first calving from September 1979 to December 1984 from 2384 herds enrolled in the Quebec Dairy Herd Analysis Service, were used to estimate genetic and phenotypic parameters of partial and total lifetime performance traits with REML. The model included herd-year-season of first calving, age at first calving, and proven sires as fixed effects and young sires and residuals as random effects. Individual lactation records were precorrected for year-month of calving before lifetime totals were calculated. Only cows with at least 5 yr of opportunity for production were analyzed. The ranges of heritability estimates were .11 to .13 for lifetime production and profit, .07 to .09 for measures of longevity, and .28 to .32 for yield per day of productive life. Correlations among total lifetime yield and profit traits and among measures of longevity were > or = .93. Genetic and phenotypic correlations, respectively, of early partial (two parities total) with total life-time yield and profit and longevity traits ranged from .81 to .94 and .66 to .78. Selection on early performance seems to be desirable and, given the high positive genetic correlations, should increase both lifetime yield and longevity.

Animals↗

Estimates of repeatability of reproductive measures in Canadian Holsteins.

Repeatabilities and heritabilities of days to first service, days open, and number of services per conception were estimated from 235,589 records on 80,333 Holstein cows, daughters of 306 sires obtained from the Quebec Dairy Herd Analysis Service, by REML. The model for statistical analyses included herd-year-season as a fixed effect and sire, cow (sire's daughter) within sire, and error as random effects. Variance components corresponding to sire, cow within sire, and error were then estimated by REML. Heritability estimates, obtained from the sire variance component, were .03, .05, and .03 for days to first service, days open, and number of services per conception, respectively. Repeatability estimates, obtained from the sire and cow within sire variance components were .08, .10, and .07 for days to first service, days open, and number of services per conception, respectively.

Animals↗

Genetic correlations between lifetime production and linearized type in Canadian Holsteins.

Genetic and phenotypic correlations were estimated between 6 lifetime production and 28 linearized type traits using REML. The data set contained 34,322 cows, each with a record for all 34 traits. The analyses accounted for the fixed effects of herd, year-month, classifier, age at first calving, and stage of lactation. Heritabilities were low for lifetime traits and moderate for most type traits except stature, size, capacity, thurl width, and pin setting, which had high heritabilities. Most phenotypic correlations between lifetime production and type were in the range of .15 to .20 except for capacity, rump, and feet and legs, which were around .07. Genetic correlations were strong between lifetime production and angularity (.44 to .55) and dairy character (.53 to .56). Genetic correlations were low to moderate between life-time production and stature (.14 to .25), size (.07 to .18), texture (.19 to .26), style (.11 to .27), head (.15 to .23), pin setting (.10 to .16), rear udder (.19 to .25), and rear attachment (.10 to .22). The only notable negative genetic correlations were lifetime production with rear heel (-.16 to -.27), thurl width (-.18 to -.24), and fore udder (-.05 to -.11).

Animals↗

Estimation of repeatability of calving ease in Canadian Holsteins.

Restricted maximum likelihood was used to estimate repeatabilities and heritabilities for calving ease from a data set of 636,972 Holstein parturitions obtained from the Quebec Dairy Herd Analysis Service. Subsets of the data, chosen to reflect different stages of cow maturity, were analyzed separately and compared. Initial least squares analyses were used to define the fixed effects to include in the model. The effects of herd-year-season, sex of calf, parity, and sex by parity interaction were significant in all subsets. Age and parity by age interaction were significant in subsets with primarily younger animals. Variances for sire of calf, cow, and residual effects were then estimated by REML. Repeatabilities ranged from 5.98 to 7.87% and heritabilities for calving ease as a direct effect ranged from 1.97 to 4.70%.

Age Factors↗

Correlations between udder conformation traits and somatic cell count in Canadian Holstein cows.

Heritabilities of, and genetic and phenotypic correlations between, type traits and the arithmetic mean of lactational SCC were estimated using minimum norm quadratic unbiased estimation and two multitrait REML methods. Three sets of data were analyzed. Heritabilities of all traits were small. For SCC, heritabilities varied between .09 and .11 and for type traits, between .08 and .14. Genetic correlations between SCC and type traits varied between -.22 and .30. Phenotypic correlations were very low. In general, correlations indicate a rather favorable association between SCC and udder conformation traits; that is, a desirable score on type would be associated with low SCC in milk.

Animals↗

Relationships of calving ease with type traits.

Type information collected by the Holstein Association of Canada was combined with calving ease data from the Quebec Dairy Herd Analysis Service. Type traits considered were overall score, general appearance, dairy character, capacity, rump, rump thurl width, rump pin setting, and set of rear legs. Calving ease was considered as a direct effect and as a maternal effect in both heifers and adult cows. After editing there were 24,618 type records, 47,023 direct effect calving ease records, and 37,068 maternal effect calving ease records from 107 sires in the analyses of heifer data. there were 26,996 type records, 16,4726 direct effect calvin ease records, and 45,261 maternal effect calving ease records in the analyses of calving involving adult cows. Multiple-trait REML was used to estimate genetic correlations between calving ease and type. The heritability of calving ease in heifers was approximately 4%, for both the direct and maternal effect, and in adult cows was approximately 1.5%. Heritability of the type traits ranged from 4 to 45%. There was a tendency for the genetic correlations between type and the direct effect of calving ease to be opposite in sign to the genetic correlations between type and the maternal effect of calving ease and for the absolute value of the correlations to be lower in adult cows than in heifers.

Analysis of Variance↗

Correlations between production traits in first lactation Holstein cows.

Genetic and phenotypic correlations between milk yield, fat (yield and percent, protein (yield and percent), and somatic cell count in first lactation Holstein cows were estimated using a multivariate restricted maximum likelihood algorithm. There were 18,189 daughters of 257 sires in 928 herds. Genetic correlations between pairs of yield traits were all positive (.73 to .88), but phenotypic correlations with somatic cell count were small and negative. Genetic correlations between somatic cell count, and fat percent, and protein percent were negative, -.11. Milk yield, fat yield, and protein yield had heritabilities of .36, .38, and .25.

Animals↗

Correlations of various direct and maternal effects for calving ease.

Multitrait restricted maximum likelihood methods were used to estimate heritabilities of direct and maternal effects for calving ease and their genetic correlation for heifers and cows. Heritabilities were 4.9% for direct effects and 4.8% for maternal effects of heifers and 1.1% and .7% for cows. Genetic or direct maternal correlations were--.40 in heifers and .07 in adult cows. Restricted maximum likelihood methods also were used to estimate the genetic correlation (.995) between direct effects for calving ease measured in heifers and in cows.

Animals↗

Correlations between calving ease and calf survival.

Multitrait restricted maximum likelihood methods were used for estimation of heritabilities and genetic and phenotypic correlations between calving ease and calf survival. These analyses examined both direct and maternal effects. Genetic correlation for direct effects between calving ease and calf survival was approximately -.8 in both heifers and cows, indicating favorable associations, whereas phenotypic correlations were -.32 and -.22.

Animals↗

Effect of sire of fetus on subsequent 90-day production of the Holstein dam.

Data on calving ease and 90-day milk, fat, protein, fat percent, and protein percent were available on 8,817 Holstein cows enrolled in the Quebec Dairy Herd Analysis Service. These observations were distributed in 802 herds, and they represented 124 service sires. Estimates of the variance components associated with the service sire (sire of fetus) were obtained by Minimum Norm Quadratic Unbiased Estimation procedures. Two models were fitted; one model included herd, month of calving, age of cow, and sire of cow as fixed effects and service sire and residual as random effects, whereas a second model included calving ease as an additional fixed effect. Variance components and percentages of variance accounted for by service sire were similar under both models. Proportions of variance accounted for by service sire were 1.1, .3, .5, .2, and .3% for 90-day milk, fat, protein, fat percent, and protein percent.

Animals↗

Components of variance and covariance between direct and fetal effects in production traits.

The correlation between sire of fetus effect and sire of cow effect on three production traits - milk yield, fat yield, and protein yield - in first lactation cows was investigated. Restricted maximum likelihood techniques were used. There was a small sire of fetus variance for milk yield, h2 .23%, while the sire of fetus variances for fat and protein were very small and negative. For milk yield the genetic correlation between sire of fetus effect and sire of cow effect was -.82. The negative sire of fetus variances for fat and protein precluded calculation of genetic correlations, both with sire of cow effects for fat and protein, and with each other and with milk yield for sire of fetus effects.

Animals↗