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R I WISE

Publications and source records attributed to R I WISE.

At least 19 recordsLinked to original sources
PubMed1963

ABSCESS-FORMING FACTOR(S) PRODUCED BY STAPHYLOCOCCUS AUREUS. II. ABSCESS FORMATION AND IMMUNITY BY A STAPHYLOCOCCUS AND ITS MUTANTS.

Lam, Gow T. (Jefferson Medical College, Philadelphia, Pa.), Francis J. Sweeney, Jr., Charlotte M. Witmer, and Robert I. Wise. Abscess-forming factor(s) produced by Staphylococcus aureus. II. Abscess formation and immunity by a Staphylococcus and its mutants. J. Bacteriol. 86:87-91. 1963.-Three mutants were derived from a virulent parent strain of Staphylococcus aureus by ultraviolet irradiation. One mutant retained coagulase but lost its hemolytic activity; a second had no coagulase but retained its hemolysin; and a third contained hemolysin and bound coagulase but no free coagulase. The parent strain was coagulase-positive, hemolysin-positive, and possessed all the known extracellular products. All four strains were compared by bacteriophage typing, mannitol utilization, and antibiotic sensitivity tests. The four isolates were studied for their pathogenicity in Swiss albino mice and four strains of inbred mice. Their immunological characteristics as studied by repeated subcutaneous injections are reported. The importance of hemolysin and the coagulase in abscess formation is discussed. Local immunity was produced only by the parent strain, but abscesses were produced by all the strains containing coagulase.

Abscess↗
PubMed1963

ABSCESS-FORMING FACTOR(S) PRODUCED BY STAPHYLOCOCCUS AUREUS. I. COLLODION BAG IMPLANTATION TECHNIQUE.

Lam, Gow T. (Jefferson Medical College, Philadelphia, Pa.), Francis J. Sweeney, Jr., Charlotte M. Witmer, and Robert I. Wise. Abscess-forming factor(s) produced by Staphylococcus aureus. I. Collodion bag implantation technique. J. Bacteriol. 86:611-615. 1963.-A collodion bag with a pore size sufficiently large to permit the diffusion of the staphylococcal extracellular products, but small enough to retain bacterial cells, was developed from a mixture of collodion and flexible collodion. These collodion bags were filled with various staphylococcal components and bacterial cells, and then implanted for 5 days into the peritoneal cavities of Swiss albino mice and four strains of inbred mice. It was found that live organisms, cell wall, and extracellular products promote an abscesslike formation around the collodion bags. The bags were surrounded with a thick layer of fibrous tissue and infiltrated with leukocytes. The exudate was free from bacterial cells. In contrast, no encapsulation occurred when the bags were filled with sterile culture medium, saline, or extracellular products which were free from coagulase after filtration through a Millipore filter.

Abscess↗