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R Ihle

Publications and source records attributed to R Ihle.

18 recordsLinked to original sources
PubMed1992

A short multilingual quality of life questionnaire--practicability, reliability and interlingual homogeneity.

International cooperative clinical trials require a multilingual quality of life questionnaire. The 'International Society for Chemo- and Immunotherapy' therefore designed a study to develop and test a 'health accentuated' quality of life questionnaire in the eight languages spoken in this society. The objective was to examine practicability, reliability and interlingual homogeneity. Versions of the questionnaire in Czech, German, Hungarian, Italian, Kroatian, Polish, Romanian and Slovakian have been prepared. The results are based on data of 1,104 adult patients. They demonstrate that the developed questionnaire is practicable. Patients need 10-15 min to answer it, usually without assistance. The proportion of missing values is for all but two questions less than 3%. The questionnaire also found high acceptance reflected by only 2.7% refusals. It is reasonably reliable in each language. Interlingual homogeneity could be shown by demonstration of strong structural similarities between the different versions using multidimensional scaling, factor analysis and comparison of mean profiles.

Adolescent↗
PubMed1989

Phenotype switch in acute leukemia patients after intensive chemotherapy.

According to Stass et al. the percentage of a lineage switch occurs in 6.7%-8.6% of patients with acute leukemia. Mostly, a conversion from the lymphoid to the myeloid phenotype is seen. In our three cases we found two switches from the myeloid to the lymphoid phenotype and only one from lymphoid to myeloid. This lineage switch is seen in relapsing and resistant leukemia cases. Different hypotheses have been discussed concerning the phenotype switch. Cytostatic chemotherapy may eradicate one leukemic cell clone, allowing another one to proliferate. Otherwise, the leukemic transformed stem cell could be influenced by the chemotherapy, resulting in a change of the differentiation program of the cell and following with a switch of marker expression. Perhaps there is some clinical importance to monitoring the phenotype switch in order to administer the best treatment.

Adolescent↗
PubMed1989

Sensitivity of CFU-GM from normal human bone marrow and leukaemic clonogenic cells (CFU-L) from blood of patients with myelogenous leukaemia to 3'-deoxy-3'-fluorothymidine in comparison to 3'-azido-3'-deoxythymidine.

3'-Deoxy-3'-fluorothymidine (FT), a thymidine analogue highly effective against HIV 1 in vitro was investigated as to its in vitro effect on normal human bone marrow CFU-GM (agar colony assay) and on human peripheral myeloid leukaemic clonogenic cells (CFU-L, colony assay in methylcellulose). For comparison, 3'-azido-3'-deoxythymidine (AZT), structurally related and used in AIDS treatment, was included in the study. Both compounds inhibit the formation of clusters and colonies from bone marrow stem cells with an [IC]50 between 10(-6) and 10(-5)M. In concentrations only 5-10 times lower than the [IC]50, FT begins to stimulate cluster and colony formation. AZT and FT also inhibit the formation of clusters and colonies from CFU-L. Compared to CFU-GM, CFU-L were more sensitive to FT, and a stimulation was not seen. It is concluded that similar side effects on bone marrow could be expected for possible use of FT against AIDS as have been found for AZT and that both compounds are potential candidates for anti-leukaemic drugs.

Adolescent↗
PubMed1989

Bone marrow stromal culture from patients with myelodysplastic syndromes and patients at different stages of acute nonlymphocytic leukaemia.

The haematopoietic microenvironment or stroma plays a decisive role for the proliferation and differentiation of haemopoietic cells. We studied if bone marrow cells from patients with myelodysplastic syndromes (MDS) and acute nonlymphocytic leukaemias (ANLL) are altered in their ability to form adherent stromal layer with active haemopoiesis in the Dexter liquid culture. Bone marrow cells were obtained from 24 normal volunteers, 28 patients with ANLL in different stages of the disease and 9 patients with MDS. There are no differences between the stromal layers of patients with ANLL in complete remission and those of normal volunteers after two weeks of cultivation. However, bone marrow cells from patients with ANLL before treatment and from patients in relapse formed a poor adherent stromal layer in most cases. In 6 of 9 cases we found the normal stromal grade of bone marrow cells from patients with MDS. There were qualitative differences in the nonadherent cell population between normal and ANLL patients in complete remission. In most cases we found morphologically recognizable erythroid cells after two-weeks Dexter liquid culture of bone marrow cells from patients with ANLL in complete remission, which were not seen with normal volunteers. This could be an indication of harmful effects on the balance of haematopoiesis caused by previous infiltration with leukaemic cells or/and high-dose chemotherapy.

Bone Marrow Cells↗
PubMed1988

[Evaluating the success of blood transfusion with erythrocytes preserved in different ways].

In a clinical study 18 patients with an indication to polytransfusion were examined by reason of secondary anaemias in diseases of the haemopoietic system. The efficiency of transfusion on the basis of body height, body mass and sex of the patients and the blood volume resulting from it as well as the exact amount of haemoglobin provided by stored blood and the increase of HB post transfusionem connected with it were found to be the criteria for the success of a transfusion. However, selected clinical parameters (LDH, Hbe, potassium, bilirubin) were constantly checked. The following values for the average efficiency of transfusion were found 16 hours post transfusionem: in washed erythrocyte concentrate 77%; in erythrocyte concentrate c. b. c. 45%; in erythrocyte concentrate GK 36%. Therefore, special emphasis is placed on an accompanying control of haemotherapy.

Blood Preservation↗
PubMed1988

[Effect of cytosine arabinoside on the differentiation of granulocyte-monocyte progenitors (CFU-GM) and myeloid leukemia blasts (CFU-L) in vitro].

The serum concentrations of Ara-C are in the range from 10(-6) to 10(-8) M in LD-Ara-C treated patients. The growth of CFU-GM from bone marrow of healthy volunteers was depressed depending on Ara-C-concentration applied in vitro. The growth of CFU-L from peripheral blood of two patients with AML (M 2) and one patient with CML in blast crisis was differently influenced by Ara-C-application in vitro. An elevated proportion of mature cells was observed in smears of cultured cells with Ara-C from two patients. The usefulness of Ara-C for a differentiation inducing therapy is discussed.

Cell Differentiation↗
PubMed1978

[Methods and techniques of sampling and performing of cytologic lymph node smears (author's transl)].

Within the diagnostics of lymphoma the indication for cytodiagnosis and the coice of gaining material and its preparation (punction and exstirpation of lymph nodes with making smears in a squeezing manner to effect simulated tissue pattern) are the basis of an appropriate and effective work of the cytologist. The good diagnostic efficiency of the cytodiagnosis of lymph nodes is in no proportion to the totally insufficient application of those methods by physicians of various medical disciplines in the hospitals and outpatient departments. The lack of an adequate teaching of students and postgraduates is a main reason for the described situation. In order that each physician may have the possibility to apply the cytodiagnosis of lymph nodes within his diagnostic programms, some special references are given for the gaining and preparation of lymph node material.

Cytodiagnosis↗
PubMed1978

[Non-secreting plasmacytomas].

In non-secernent plasmocytomas there are no characteristic changes of serum protein. Diagnostic difficulties may be overcome by cytomorphological examinations, immunofluorescence and electron microscopy. By referring to 4 own observations the value of the electron microscopic characteristics of plasma cells is demonstrated. Subtile investigations in protein diagnostics are required for avoiding incomplete monoclonal immunoglobulins which may greatly enter the kidneys to be overlooked. Generally the prognosis of non-secernent plasmocytomas is not worse than that of other forms.

Aged↗
PubMed1977

[Bone-marrow insufficiency].

In a survey the definition of the notions insufficiency of the bone marrow, panmyelopathy, panmyelophthisis and aplastic anaemia, the subdivision into congenital and acquired forms as well as to the clinical course, the epidemiologic situation as well as the distribution according to age and sex are described. More in detail the author enters the etiological factors, in which cases the etiological classification of the individual case may be connected with considerable difficulties. Exogenic noxae are subdivided into factors dependent on dose and independent on dose as pathogenetic principles. In this connection professional noxious substances as well as unwished side-effects of medicaments are discussed more in detail. Despite all variety the possibilities of therapy are limited.

Adolescent↗
PubMed1975

[Hematologic function tests in early diagnosis].

The functional tests in form of tolerance tests allow an insight into the adaptibility of the organism to changed inner and outer conditions. The majority of haematological functional tests demands a temporary and economic expediture which excludes it a priori as screening test. According to a previously published phase program for the haematological functional diagnosis a report is given on simple methods for judging the disturbed partial functions of the blood.

Adaptation, Biological↗
PubMed1975

[The significance of morphologic-hematologic methods].

A survey is given on the value of the haematologo-morphological examination methods, especially the differential counting of leucocytes, the smear of a concentrate of leucocytes and the cytomorphological judgment of the bone marrow. None of these methods is suitable for the search for cases in the sense of a preliminary diagnosis. This is also of importance especially for the differential counting of leucocytes. However, with aimed methods of proceeding these methods are of great diagnostic value, so that they may be very much in front in a graduated diagnostic program. The diagnostic evidence of a cytomorphological preparation of the bone marrow must highly be estimated concerning a situation of deficiency of iron, a diffuse or small nodular metastasizing of the bone marrow or lesion of the bone marrow by exogenic factors.

Bone Marrow Examination↗