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R J Billing

Publications and source records attributed to R J Billing.

35 records · Page 2Linked to original sources
PubMed1976

The absence of B-cell antigen B2 from leukaemia cells and lymphoblastoid cell lines.

60 leukaemia patients with acute mylogenous leukaemia, chronic myelocytic leukaemia, acute lymphatic leukaemia, and chronic lymphocytic leukaemia were tested for five specificities of the new B-lymphocyte alloantigenic system. Two specificities of the first B-cell locus were significantly lower in frequency than in B lymphocytes from 105 controls. B-cell group 1 was found in 18% of leukaemia patients vs. 48% of controls (p less than 0.025) and B-cell group 2 was found in 0% of leukaemia patients vs 23% of controls (p less than 0.025). Group 2 was also absent in 37 cultured lymphoblastoid lines. It is suggested that resistance to B-cell leukaemia and to Epstein-Barr virus transformation in vitro may be linked to a B-lymphocyte specificity on the first B-lymphocyte locus.

Adult↗
PubMed1976

Blocking of HLA and B lymphocyte alloantigens with F (ab')2 fragments of rabbit antibodies.

F(ab')2 fragments of rabbit antibodies specific for human B lymphocytes will block human anti-B lymphocyte alloantibodies. F(ab')2 of anti-beta2m will block HLA alloantibodies. The F(ab')2 fragments can be used to improve the typing of B cell lines and certain leukemia cells which express both HLA and B lymphocyte alloantigens. Pretreatment of these cells with anti-beta2m F(ab')2 preparations allows the observation of B cell alloantigen reactions independently of HLA reactions. Similar use of rabbit anti-B cell F (ab')2 allows HLA reactions to be observed free from the "extra reactions" caused by B alloantibodies that are present in many HLA typing sera.

Animals↗
PubMed1976

Isolation and characterization of human B cell alloantigens.

Human B lymphocyte alloantigens were solubilized from malignant spleen cell membranes from lymphoma patients by detergent treatment or papain digestion. Antigenic activity was detected by inhibition of cytotoxicity of rabbit and human anti-B cell antisera. Extracts from one patient (AC) specifically inhibited only B alloantisera of B groups 1 and 3; sera from B groups 4 and 5 were not inhibited. Inhibitory titers were greater than 1:400 against positive sera and less than 1:2 against negative sera. The papain extracts were purified by QAE-A50 Sephadex and Concanavalin A Sepharose 4B chromatography, and preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis (PAGE) under nondenaturing conditions. By testing gel slices for antigenic activity the m.w. of the papain-extracted B antigen was 58,000 whereas the detergent-extracted antigen was 65,000. Antigenic activity detected by rabbit and human antisera copurify together, suggesting that these sera were reacting with the same molecules. Immunoprecipitation of sodium deoxycholate-solubilized extracts of a cultured human B lymphoid cell line 8392 with the rabbit anti-B cell antisera revealed two B cell polypeptides of apparent m.w. 27,000 and 35,000 that were not found on the paired T line 8402. It is suggested that these polypeptides might be subunits of the 65,000 dalton native B lymphocyte alloantigen. The polypeptide subunits do not appear to be linked covalently by disulfide bonds.

B-Lymphocytes↗
PubMed1969

Changes in the patterns of synthesis of ribonucleic acid species in immature rat uterus in response to oestrdiol-17 beta.

1. After treatment of immature rats with diethylstilboestrol, the wet weight and RNA content of uterine tissue increased rapidly, reaching a peak at 40hr. After an initial lag of a few hours, the acid-soluble ribose and protein contents also rose to maxima at 40hr. No increase in DNA content occurred until at least 24hr. after treatment. 2. The RNA from immature rat uterus isolated at various times up to 6hr. after administration of oestradiol-17beta was labelled by injecting [(3)H]uridine and [(3)H]guanosine intraperitoneally 30min. before the animals were killed. It was fractionated on columns of kieselguhr coated with methylated serum albumin and the radioactivity in fractions corresponding to transfer RNA, 7s RNA, ribosomal RNA, Q(1)-RNA, Q(2)-RNA and DNA-like RNA was determined. 3. The radioactivity of the whole RNA increased steadily for 6hr. after hormone treatment. The earliest changes occurred in the Q(1)-RNA (ribosomal RNA precursor), whereas at longer time-intervals the radioactivity of the ribosomal RNA, 7s RNA and transfer RNA increased by four- to five-fold. The radioactivity of the DNA-like RNA increased by about 50%, but only at the longer time-intervals. 4. It is concluded that one of the earliest changes in response to oestradiol treatment is a major increase in synthesis of ribosomal RNA followed later by a similar increase in synthesis of transfer RNA and by a much smaller increase in synthesis of DNA-like RNA. The change in synthesis of ribosomal RNA in immature rat uterus may represent one of the most important responses to oestradiol treatment.

Animals↗