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Biomedical subjects

R J Heddle

Publications and source records attributed to R J Heddle.

26 records · Page 2Linked to original sources
PubMed1980

Specific IgM and IgG antibodies in IgA deficiency.

Evidence of an abnormality of IgM- and IgG-specific antibody responses was sought in subjects with selective IgA deficiency. Twenty-four patients having a serum IgA level less than 0 . 37 g/l were detected by local-population screening. Total IgM and IgG levels were measured as well as specific antibody to the lipopolysaccharides of six 0 serotypes of E. coli which are frequently isolated from human faeces. Serum IgM-class E. coli antibodies were significantly lower in IgA-deficient patients than in age- and sex-matched controls. The respective IgG-class antibody was generally elevated although more variation existed in this class. There was no correlation of specific antibody in either class with the degree of IgA deficiency. Possible explanations for these patterns of antibody response are discussed. It is concluded that some subjects with 'selective IgA deficiency' may be deficient in specific IgM antibody responses to certain antigens.

Antibody Specificity↗
PubMed1978

In vitro degradation of mouse, rabbit and dog antibodies to Vibrio cholerae by succus entericus.

Purified antibodies to Vibrio cholerae from mouse, rabbit and dog were digested in vitro by homologous intestinal secretions. When assessed with regard to their complement-dependent vibriocidal activity, IgG antibodies were generally more susceptible to degradation than IgM antibodies, High levels of tryptic inhibitors were required to inhibit this digestion. Rabbit IgG was unusual in being quite resistant to digestion. Gel filtration studies demonstrated that secretory IgA, isolated from mouse intestinal secretions, was resistant to proteolysis. Similar studies on dog IgG and mouse IgM demonstrated production of F(ab') 2-like fragments. Digestss of these antibodies, while devoid of Fc-mediated vibriocidal activity, retained significant protective activity for baby mice.

Animals↗
PubMed1978

The source of IgM and IgG in the dog intestine.

The source of IgM and IgG2a,b in the dog intestine was investigated, using labelled dog immunoglobulins and chronic Thiry-Vella loops of dog small intestine. Clearancess of IgM and IgG2a,b from serum into intestinal secretions were similar, reflecting transfer of a greater mass of IgG2a,b than of IgM. Only 20% of the intestinal IgM derived from serum, irrespective of the marker used. About one third of the total intestinal IgG, of all sub-classes and accrued from all sources, was serum-derived IgG2a,b. This indicates that serum is a major contributor to dog intestinal IgG2a,b.

Animals↗
PubMed1975

The effect of lysozyme on the complement-dependent bactericidal action of different antibody classes.

Preparations of rabbit, dog and sheep IgA, IgA and IgM were examined for their antibacterial effects using a complement-dependent bactericidal assay. IgM and IgG were efficient bactericidal antibodies in the presence of complement; IgA, however, contained negligible activity. Except for sheep IgG no enhancement of bactericidal activity was observed in the presence of added lysozyme.

Animals↗
PubMed1975

Dog immunoglobulins. II. The antibacterial properties of dog IgA, IgM and IgG antibodies to Vibrio cholerae.

Secretory IgA antibodies to Vibrio cholerae were purified from the parotid saliva and mammary secretions of locally and orally immunized dogs using gel filtration, ion-exchange chromatography and anti-immunoglobulin immuno-absorbents. IgM and IgG antibodies were isolated from serum by gel filtration and ion-exchange chromatography. IgA antibodies proved to have minimal, if any, activity in direct killing of bacteria in the presence of complement or in the promotion of phagocytosis. The minimal activity which IgA had in these assays could be accounted for by extremely small quantities of IgM antibody. The same IgA antibodies, mixed with the challenge innoculum of Vibrio cholerae and fed to infant mice, protected these mice as efficiently as IgG or IgM antibodies.

Animals↗
PubMed1975

Dog immunoglobulins. I. immunochemical characterization of dog serum, parotid saliva, colostrum, milk and small bowel fluid.

The levels of immunoglobulins A, M and G were measured in dog serum, colostrum, milk, parotid saliva and small bowel fluid using the single radial immunodiffusion method. All the external secretions except early colostrum, by contrast with serum, were found to be rich in IgA with small quantities of IgM and IgG. Exocrine immunoglobulins were partially characterized by gel filtration.

Absorption↗
PubMedDate not supplied

Fatal anaphylaxis following jack jumper ant sting in southern Tasmania.

The "jack jumper" ant (Myrmecia pilosula) is a major cause of anaphylaxis in Tasmania. We describe four deaths attributed to stings by this ant between 1980 and 1999. All victims were men aged 40 years or over with significant comorbidities; two were taking angiotensin-converting enzyme inhibitors, which may increase risk of severe anaphylaxis. Three victims had known ant-sting allergy, but only one carried adrenaline, which he did not use. Another believed he was protected by previous attempts at hyposensitisation with whole ant-body extract. There is potential to prevent deaths by careful education of people with known allergy, prescribing of adrenaline for auto-injection and development of an effective hyposensitisation therapy.

Adult↗