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R J Hodges

Publications and source records attributed to R J Hodges.

7 recordsLinked to original sources

Responses of Teretrius nigrescens toward the dust and frass of its prey, Prostephanus truncatus.

Teretrius nigrescens is considered to be a specialized predator of Prostephanus truncatus, a serious pest of stored maize and dried cassava roots. Using a bait-bag behavioral bioassay, this investigation found T nigrescens to be strongly arrested by dust and frass produced by P. truncatus on maize, whereas responses to maize flour were weak. Attempts to increase the arrestiveness of flour by altering its physical properties (coarseness or particle size range) were unsuccessful. The arrestive property of dust/frass did not degrade with long-term storage nor did it volatilize or degrade with oven baking up to 150 degrees C. However, extraction with methanol and reapplication was successful in transferring the arrestive property from the dust/frass onto maize flour. The prey dust/frass also induced more oviposition than did maize flour, as judged by production of F1 offspring. The results provide strong evidence for the existence of a high boiling point compound(s) in P. truncatus dust/frass that acts as a contact kairomone for T nigrescens. This, or another compound(s) also seems to act as an oviposition stimulant for female T. nigrescens.

Animals↗

Phenotypic plasticity of Rhyzopertha dominica pheromone signaling: the effects of different hosts and presence of conspecific females on male produced aggregation pheromone.

Male Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) release an aggregation pheromone consisting of a blend of two components, dominicalure 1 (D1) and Dominicalure 2 (D2). Pheromone from single insects, in different contexts, was collected and measured to determine if this signal is phenotypically plastic. Release rates were lowered when males were moved from maize grains to groundnut kernels or when moved from solitary occupation of maize grain to grain occupied by seven females. The pheromone release was increased again once these moves were reversed. The release of D1 was more affected than D2: thus, on groundnuts or in the presence of females, less pheromone was released and the proportion of D1 in the blend was lowered. Possible reasons for the modifications of the signal are discussed.

Animal Communication↗

Formation of LID vector complexes in water alters physicochemical properties and enhances pulmonary gene expression in vivo.

There is currently an urgent need to develop efficient gene-delivery systems for the lung that are free of inflammatory effects. The LID vector is a synthetic gene delivery system, comprised of lipofectin (L), an integrin-targeting peptide (I) and DNA (D) that has previously been shown to have high transfection efficiency in the lung. We have assessed the effect of alternative methods of complex preparation on structural features of the complex, levels and duration of reporter gene expression and the host response to the LID vector. We have demonstrated that making the complex in water affects the structure of the LID complexes making them smaller and more stable with a more cationic surface charge than complexes prepared in phosphate-buffered saline (PBS). When the LID vector was constituted in water and instilled intratracheally into the lungs of mice there was a 10-fold increase in luciferase activity compared with preparation in PBS. Furthermore, luciferase activity was still evident 1 week following vector instillation. This enhancement may be because of altered complex structure, although effects of the hypotonic vector solution on the lung cannot be excluded. The inflammatory effects of instilling the LID vector in water were minimal, even after three administrations of the LID vector, with only mild alterations in cytokine and broncho-alveolar lavage fluid (BALF) cell profiles. These results demonstrate that the LID vector can generate high, and prolonged, levels of gene expression in the lung from small quantities of DNA and that careful attention to synthetic polyplex structure may be important to optimize efficiency of gene expression in vivo.

Animals↗

Intermale variation in aggregation pheromone release in Prostephanus truncatus.

Intermale variation in pheromone signaling has been confirmed and quantified by measurements of pheromone produced by single adult male Prostephanus truncatus (Horn) (Coleoptera: Bostrichidae). Males varied in both the amounts of the two components of their aggregation pheromone and the ratio of one component to the other. The mean rates of production of the pheromone components T1 and T2 were 1.9 and 0.5 microg/day, respectively. There were repeatable differences among males in the amounts of T2 produced and the proportion of T1 in the pheromone blend over two weeks. Of the 15 males studied, one released a large burst of pheromone in a short period, while the remainder, if they did release, did so over an extended period. This suggested that there may be two alternative release strategies and the significance of this is discussed.

Animals↗

The relationships among palpator experience, milk progesterone concentration and estrus and fertility in cows with palpable corpora lutea treated with cloprostenol.

Two hundred and fifty-one cows fresh at least 37 days with normal reproductive tracts and palpable ovarian structures which clinicians believed to be corpora lutea and which were presumed to be producing progesterone were treated with 500 micrograms of cloprostenol. The cows were stratified into three groups based on milk progesterone concentrations in whole milk samples taken at the time of examination. The low group had milk progesterone concentrations less than or equal to 1 ng/ml, the intermediate group had milk progesterone concentrations between 1 and 3 ng/ml, and the high group had milk progesterone concentrations greater than or equal to 3 ng/ml. The proportion of cows in each group inseminated within 5 days of treatment and the fertility at that breeding were compared. There were no significant differences among the groups with respect to the proportion of cows that came into estrus within 5 days. However, the conception rate of the high group (54%) was significantly greater than that of the intermediate group (26%). To study the relationship between practitioner experience and diagnostic accuracy the proportion of cows with low, intermediate and high concentrations of progesterone selected by clinicians with more than 3 years experience was compared to that for clinicians with 3 years of experience or less. Less experienced clinicians were significantly more likely to make a false positive diagnosis of functional luteal tissue in cows with palpable ovarian structures than were more experienced clinicians.

Animals↗

Lead poisoning by a mug.

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Cooking and Eating Utensils↗