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R J Lanzafame

Publications and source records attributed to R J Lanzafame.

At least 19 recordsLinked to original sources
PubMed2004

Acute tensile strength analysis of collagen solder for mesh fixation to the peritoneal surface.

BACKGROUND: In this study, we assessed the feasibility of laser-assisted tissue welding as a means of fixing mesh prostheses to the peritoneum. We then tested the initial tensile strength of the bonds. METHODS: Fresh porcine peritoneal coupons were lap-joint bonded with laser-activated solder. Anesthetized New Zealand white rabbits and Yorkshire pigs also underwent laparotomy. Vicryl mesh (2.0 x 1.0 cm) was attached to the peritoneum using a laser system (1.43 mu, 2.5 W, 60 degrees C), solder formulations and configurations, and a 1 cm2 bond area. Control segments were affixed with 4.8-mm staples. After the animals were killed, the segments were excised en bloc. Tensile strength assessment was conducted by measuring peak force breaking strength. RESULTS: The strength of the solder bonds were similar in all groups (range, 261.5 +/- 170.3-465.3 +/- 288.2 g/cm2) and were not statistically different from the controls (215.8 +/- 117.8 g/cm2). CONCLUSIONS: These values are similar to the 200-500 g/cm2 acute strengths reported for sutured or stapled peritoneal closure. Mesh fixation by solder is feasible, and further development of this technology is warranted.

Animals↗
PubMed2001

830-nm irradiation increases the wound tensile strength in a diabetic murine model.

BACKGROUND AND OBJECTIVE: The purpose of this study was to investigate the effects of low-power laser irradiation on wound healing in genetic diabetes. STUDY DESIGN/MATERIALS AND METHODS: Female C57BL/Ksj/db/db mice received 2 dorsal 1 cm full-thickness incisions and laser irradiation (830 nm, 79 mW/cm(2), 5.0 J/cm(2)/wound). Daily low-level laser therapy (LLLT) occurred over 0-4 days, 3-7 days, or nonirradiated. On sacrifice at 11 or 23 days, wounds were excised, and tensile strengths were measured and standardized. RESULTS: Nontreated diabetic wound tensile strength was 0.77 +/- 0.22 g/mm(2) and 1.51 +/- 0.13 g/mm(2) at 11 and 23 days. After LLLT, over 0-4 days tensile strength was 1.15 +/- 0.14 g/mm(2) and 2.45 +/- 0.29 g/mm(2) (P = 0.0019). Higher tensile strength at 23 days occurred in the 3- to 7-day group (2.72 +/- 0.56 g/mm(2) LLLT vs. 1.51 +/- 0.13 g/mm(2) nontreated; P < or = 0.01). CONCLUSION: Low-power laser irradiation at 830 nm significantly enhances cutaneous wound tensile strength in a murine diabetic model. Further investigation of the mechanism of LLLT in primary wound healing is warranted.

Animals↗
PubMed2001

Laser use and research in gastroenterology, gynecology, and general surgery: a status report.

OBJECTIVE AND BACKGROUND DATA: Despite the burgeoning growth of laser applications in dermatology and plastic surgery, applications in other specialties have declined. Laser use in gastroenterology, general surgery, and gynecology was examined over the past 3 years. Future trends and opportunities are discussed. METHODS: The MEDLINE database was scanned for scholarly publications between January 1, 1997, and January 1, 2000, and the number of publications in these specialties was determined. A questionnaire was distributed to 362 general surgeons from the American Society for Laser Medicine and Surgery (ASLMS) to assess current use and future needs. These results were compared to the actual cases performed at a laser center over the same period. RESULTS: Of 3,331 publications, 21 (0.6%) covered gastroenterology (GE), general surgery (GS), gynecology (GYN), or laparoscopy (LAP). Keyword citations were 2 GS, 3 GYN, 7 GE, and 12 LAP. Questionnaire results and actual utilization were well correlated. CONCLUSIONS: Lasers are being used in these specialties despite slow development of novel uses. Opportunities exist for future applications.

Adult↗
PubMed2000

In vitro effects of low-level laser irradiation at 660 nm on peripheral blood lymphocytes.

BACKGROUND AND OBJECTIVE: The effects of low-level laser light irradiation are still highly contested, and the mechanisms of its action still unclear. This study was conducted to test the effects of low-level laser irradiation at 660 nm on human lymphocytes and to investigate the possible mechanisms by which these effects are produced. STUDY DESIGN/MATERIALS AND METHODS: Whole blood obtained by phlebotomy was irradiated at 660 nm by using energy fluences between 0 and 5.0 J/cm(2). The lymphocytes were isolated after irradiation of the whole blood. For the control experiment, the lymphocytes were first isolated and then irradiated at the same wavelength and energy fluence for comparison. The proliferation of lymphocytes and the formation of free radicals and lipid peroxides were monitored. Hemoglobin was also irradiated in a cell-free environment to test for the production of lipid peroxides. RESULTS: Lymphocyte proliferation was significantly higher (P<0.05) as expressed by a Stimulation Index in samples irradiated in the presence of whole blood compared with lymphocytes irradiated after isolation from whole blood. Free radical and lipid peroxide production also increased significantly when samples were irradiated in the presence of red blood cells. CONCLUSION: The present study supports the hypothesis that one mechanism for the photobiostimulation effect after irradiation at 660 nm is the reaction of light with hemoglobin, resulting in oxygen radical production.

Blood↗
PubMed1999

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Humans↗