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Biomedical subjects

R Jankie

Publications and source records attributed to R Jankie.

7 recordsLinked to original sources
PubMed2000

Precise analysis of primary amino acids in urine by an automated high-performance liquid chromatography method: comparison with ion-exchange chromatography.

A precise, simple and rapid method for the quantitative determination of primary amino acids in urine based on high-performance liquid chromatography and o-phthaldialdehyde pre-column derivatization is described. All primary urinary amino acids could be determined within 49 min (injection to injection). Amino acid concentrations in 40 urinary samples were measured by this method and the results were compared with those measured by ion-exchange chromatography. The correlation coefficient for the common amino acids was greater than 0.90. This is the first study in which such a detailed comparison has been made on urine samples. It appeared that the method described is an excellent alternative to the classical ion-exchange method for the quantitation of urinary amino acids.

Adolescent↗
PubMed1984

Should corneas from donors receiving a high dose of salicylate be used as grafts: an animal experimentation.

Rabbits were injected intravenously with a single dose of sodium salicylate (350 mg kg-1) in order to study the following in vivo: (i) the distribution of the drug in the various eye tissues and fluids, particularly the cornea and aqueous humour, and (ii) transfer of the drug from corneal grafts obtained from donors receiving sodium salicylate to the eye tissues of the recipient. In additional experiments, the in vitro effects of sodium salicylate on the growth and protein synthesis of corneal cells grown in tissue culture were also studied. In vivo experiments showed that the periods during which we observed the highest concentration of salicylate in the serum and in the eye tissues were within 30 min and 2 hr respectively following the injection. These experiments also showed that salicylate was transferred from the treated donor via corneal graft to the recipient's eye tissues where it could still be detected 48 hr after the operation. In vitro experiments showed that a 50% inhibition of cell growth was obtained at a concentration of about 1000 micrograms salicylate ml-1 while protein synthesis was decreased by 50% at a concentration of about 200 micrograms ml-1. A consideration of our data from the in vivo and in vitro experiments together suggests that ingestion by the donor of high concentrations of salicylate may have the potentiality of subjecting the corneal endothelial cells to cytotoxic concentrations of the drug thus jeopardizing the success rate of corneal graft operations.

Animals↗
PubMed1984

Ocular distribution of sodium pentobarbital after injection of lethal and anesthetic doses and after transfer via corneal grafting.

The distribution of sodium pentobarbital in the eye after lethal and anesthetic doses and the possibility of the drug's transfer via corneal grafting were studied in rabbits. The drug was found in all the ocular tissues and humours following the intravenous injection of a single lethal (60 mg/kg) or anesthetic (30 mg/kg) dose, and it was still present 24 hours after the anesthetic injection. In both instances the largest quantity was found in the cornea. After grafting of corneal tissue containing pentobarbital the drug dispersed into the recipients' corneas and other ocular tissues. In-vitro studies showed that 250 micrograms of the drug per millilitre of incubation medium reduced the rate of growth of the corneal fibroblasts to 50%, and 300 micrograms/mL reduced their protein synthesis to 66%. Calculations showed that these concentrations could be reached in the cornea or the aqueous humour in vivo following either the lethal or the anesthetic injection of the drug.

Anesthesia↗
PubMed1983

Distribution of morphine in the eye tissues and fluids of rabbits after systemic administration and after transfer via corneal grafting.

To determine whether corneas from animals receiving morphine could be used for grafting, rabbits were given a single intravenous injection of tritium-labelled morphine and killed 10 minutes later. Of the drug entering the eye, 12% was found in the cornea, 36% in the retina and optic nerve, 29% in the uveal tissues, 8% in the aqueous humour, 8% in the choroid, 4% in the vitreous humour and 2% in the lens. Another group of rabbits then received full-thickness corneal grafts from rabbits treated with morphine. The grafts contained the drug for up to 21 days after transplantation. From the graft bed the agent dispersed into the different solid ocular tissues and intraocular fluids. None of the grafts failed, and none of the recipients' eyes showed any adverse reaction. In-vitro tests showed that the amount of morphine found in the cornea and the aqueous humour of the donor did not have any significant effect on cell division or protein synthesis of the corneal cells. Higher concentrations of the drug, however, decreased both cell division and protein synthesis.

Animals↗
PubMed1982

Distribution of penicillin in the cornea, tears and aqueous humour at various times following a single intravenous injection.

Bioassays performed by the disc diffusion technique on samples of blood, tears, aqueous humour and cornea from 30 rabbits killed 4 to 24 hours following a single intravenous injection of benzylpenicillin (40 mg/kg) showed that the penicillin content of these fluids and tissues progressively decreased with time. However, even after 24 hours the agent could be detected in the samples. The peripheral and central cornea contained similar amounts of penicillin up to 16 hours; thereafter, the peripheral cornea contained more of the agent.

Animals↗
PubMed1981

Effect of hydrocortisone on the mobilisation of leucocytes in corneal wounds.

We have studied in rabbits the effect of subconjunctivally injected hydrocortisone on the polymorphonuclear leucocyte invasion of corneal wounds at different times after an injury. One group of rabbits was treated with the steroid (hydrocortisone group) and the other not (control group). After making nonpenetrating trephine incisions on the cornea we obtained cellular samples by the impression technique at a given postoperative period (2, 4, or 6 hours), and then the animal was killed. The cornea was processed for histological study of the infiltrating cells. At any postoperative period the number of polymorphonuclear leucocytes in the corneal wounds of the hydrocortisone group was significantly less than the number in the identical wounds of the control group (p less than 0.01 to 0.001).

Animals↗