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Biomedical subjects

R K Cunningham

Publications and source records attributed to R K Cunningham.

28 records · Page 2Linked to original sources
PubMed1975

Phagocytosis as a surface phenomenon. V. Contact angles and phagocytosis of rough and smooth strains of Salmonella typhimurium, and the influence of specific antiserum.

The angle made by a drop of saline in contact with a monolayer of Salmonella typhimurium or phagocytic cells, the contact angle, is a measure of their relative interfacial tension, and is predictive of a successful phagocytosis. Smooth strains of S. typhimurium possess a contact angle lower than the phagocytic cells and resist phagocytosis. Rough strains have an angle higher than the phagocytes and are readily engulfed. The lower contact angle of smooth strains can be increased by treatment with specific antibody resulting in more efficient phagocytosis.

Animals↗
PubMedDate not supplied

Studies of the recovery and the cost of low-glycerol cryopreserved human red blood cells.

Red blood cells were equilibrated with 28 per cent (v/v) glycerol and 3 per cent mannitol in 0.65 g/100 ml sodium chloride. The units were frozen by immersion into liquid nitrogen and stored at -160 C. After thawing, they were reconstituted and washed using the IBM 2991 Blood Cell Processor. Freeze-thaw rate curves, the effect of thawing techniques, the effect of varying postthaw washing and processing techniques, estimates of red blood cell losses because of hemolysis, and in vitro recovery were determined. In vivo recovery was determined by 51Cr techniques 24 hours after infusion and Ashby survivals and subsequent life span were measured. Metabolic, scanning electronmicroscopy, cost estimates and quality control studies were done on the reconsituted red blood cells. Recipients were evaluated before and after transfusion for metabolic erythrocyte characteristics and for evidence of hemolysis. The modified method requires less wash solution and less technician time than does the standard low-glycerol method. Two units for the same recipient could be passed through the IBM software with no alteration of cell survival or loss. Revision of the IBM 2991 processing procedure provided excellent recovery of viable previously frozen red blood cells at probably a lower cost.

Blood Preservation↗
PubMedDate not supplied

Cryopreservation of complement-coated erythrocytes.

Cells coated with complement components (C3b-C4b and C4b cells) were prepared by various methods and stored in liquid nitrogen using a low-glycerol, rapid freeze technique. Freshly coated cells, and cells coated and then frozen were tested versus various antiglobulin and anticomplement reagents to evaluate the reactivity of such frozen, stored, complement-coated human red blood cells. Liquid nitrogen preservation of such coated cells proved to be feasible when such cells were compared with freshly coated control cells.

Animals↗