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Biomedical subjects

R K Lu

Publications and source records attributed to R K Lu.

9 recordsLinked to original sources
PubMed2000

Comparative study on efficacy of three sperm-separation techniques.

AIM: To evaluate the comparative effectiveness of real-time sperm separation technique (Wang's tube method) and other two conventional methods in isolating high-quality sperm preparation, and to compare the spouse pregnancy rate in intrauterine insemination (IUI) with sperm preparations isolated by these methods. METHODS: The effectiveness of the real-time sperm separation technique, the conventional swim-up and the Percoll discontinuous density gradient methods in isolating sperm preparations from 60 infertile patients (20 with apparently normal semen and 40, abnormal semen contaminated with microorganisms and other impurities) was evaluated and compared. The microorganisms to be removed included bacteria, virus, Chlamydia trachomaticum, Ureaplsama urealyticum, etc. The spouse pregnancy rates in IUI with sperm preparations isolated by these three techniques from 80 oligoasthenoteratospermic patients were also compared. RESULTS: The quality (including the percentages of normal form, normal-chromatin and motile sperm, and the grade of motility) of sperm obtained by the real-time sperm separation technique was much higher (P < 0.01) as compared with those by the other two methods. The Wang's tube method was also more effective in removing microorganisms and other impurities. The method provided a higher IUI pregnancy rate than the other two sperm separation techniques (P < 0.05). CONCLUSION: The real-time sperm separation technique is the most effective method so far available in isolating high-quality sperm samples to be used in assisted reproduction.

Adult↗
PubMed1997

[Effects of semen Cuscutae, rhizoma Curculiginis, radix Morindae officinalis on human spermatozoan's motility and membrane function in vitro].

OBJECTIVE: To observe the effects of Semen Cuscutae (SC), Rhizoma Curculiginis (RC) and Radix Morindae Officinalis (RMO) on human sperm motility in vitro and cytomembrane function. METHODS: Compare the sperm motility of the above-mentioned medicinal plants with modified Tyrode's solution (MTS) and MTS added caffeine in vitro. Human spermatozoa were incubated with decoctions of medicinal plant at various concentrations in 37 degrees C for 30 minutes. The motility of sperm was evaluated by the sperm capillary penetrating test, sperm speed test and sperm activity index calculations. The spermatozoa membrane function was evaluated by spermatozoal hypo-osmotic swelling test together with eosin staining methods. RESULTS: The sperm motility improved markedly and sperm membrane function became more stabilized after incubation, the SC had best effect, RC took second place, RMO was relatively poor. CONCLUSIONS: SC decoction is an effective promoting preparation for sperm motility and the membrane function stabilized. Therefore, SC may be beneficial in treating male sterility and may raise success rate in artificial insemination.

Drugs, Chinese Herbal↗
PubMed1989

[Clinical study on LRH-A contraception by luteolysis].

Thirty-eight healthy female volunteers, aged 26-38 years with regular menstrual cycles entered into a clinical trial for contraception with Chinese made superactive stimulatory analog of LRH i.e., (D-Ala, des-GLY-EA10)-LRH yielding luteolysis. LRH-A 1 mg a day was administered by intranasal spray from the 15th through 25th day of the menstrual cycle for 1-12 consecutive cycles. During LRH-A administration no additional contraceptive was used. Luteolysis refers to reduction in plasma progesterone and shortening of the luteal phase. The results showed that luteolysis occurred in 154/158 cycles, 4 pregnancies occurred due to faulty administration. No severe side-effects were observed during administration and apparently normal cycles occurred immediately after the withdrawal of LRH-A spray. The above data indicated that LRH-A may provide a fairly good luteolytic effect. More intensive study is required for clinical application in contraceptive practice.

Administration, Intranasal↗
PubMed1988

[Luteinizing hormone-releasing hormone (LRH) agonist in the treatment of breast cancer, osteosarcoma and prostate carcinoma--analysis of 12 patients].

This paper first reports the results of 12 patients with sex hormone-dependent neoplasms, including 9 women with breast cancer, 2 men with osteosarcoma and 1 man with prostate carcinoma, treated by LRH agonist, (D-Ala6, des-Gly-NH2(10))-LRH-ethylamide (LRH-A) 100-200 micrograms, IM, QD. After 15-30 days of administration, the concentrations of plasma mean estradiol, progesterone, testosterone, serum luteinizing hormone and follicle-stimulating hormone were lowered significantly in the peripheral blood of all patients, associating with improvement of the patients' general condition and reduction of the tumors and/or metastatic foci. No serious side effects were observed except vaginal irregular bleeding in isolated patients. Three patients died and the others were alive in follow-up of 4-30 months. The results suggest that LRH-A be useful in the treatment of the sex hormone-dependent tumors and worth further study.

Adolescent↗