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Biomedical subjects

R K Mandal

Publications and source records attributed to R K Mandal.

At least 19 recordsLinked to original sources
PubMed1991

Characterization of 2S seed storage protein of Brassica campestris and its antigenic homology with seed proteins of other Cruciferae.

The low molecular weight seed storage protein of Brassica campestris has been isolated and its amino acid composition determined. Antibody raised against this low molecular weight protein has been used to compare the antigenic similarity between the low molecular weight storage proteins of different Cruciferae seeds by immunoprecipitation and Western blotting. These studies revealed the existence of antigenically homologous proteins of identical molecular weights in seeds of other Cruciferae but absent in some other dicots like mung bean and tobacco seeds.

Amino Acids↗
PubMed1989

Organization and size class homogeneity of ribosomal RNA genes of catfish Heteropneustes fossilis.

The ribosomal RNA genes of catfish Heteropneustes fossilis Bloch were examined by Southern blot analysis of genomic DNA digested with restriction enzymes and probed with labelled catfish ribosomal RNA. The major repeat length is 12 kb and about 300 copies per haploid genome are tandemly arranged. The repeat lengths are homogeneous in size in different tissues and individuals. A restriction site polymorphism exists in some of the repeats.

Animals↗
PubMed1988

Cloning and characterization of a highly repetitive fish nucleotide sequence.

We have cloned and sequenced a highly repetitive HindIII fragment of DNA from the common carp Cyprinus carpio. It represents a tandemly repeated sequence with a monomeric unit of 245 bp and comprises 8% of the fish genome. Higher units of this monomer appear as a ladder in Southern blots. The monomeric unit has been sequenced; it is A + T-rich with some direct and some inverse-repeat nucleotide clusters.

Animals↗
PubMed1981

The nucleotide sequence at the transcription termination site of ribosomal RNA in Drosophila melanogaster.

The sequence of 300 nucleotides surrounding the transcription termination site for ribosomal RNA in Drosophila melanogaster has been determined. The precise position of the RNA 3'-end was located by sizing rDNA fragments protected from S1 nuclease by hybridization with cytoplasmic 28S rRNA or with nuclear 38S pre-rRNA. Next, the sequence of the first 10 nucleotides at the 3'-end of 28S and 38S rRNA was determined directly. The results indicate the absence of 3'-terminal processing of the 38S pre-rRNA. The nucleotide sequence from a position 27 nucleotides upstream to 150 nucleotides downstream from the termination site was identical in three uninterrupted rRNA genes and one interrupted gene, except for a single base change in the spacer region of one clone. The terminal 100 nucleotides of Drosophila 28S rRNA have more than 60 per cent homology with the corresponding region of Xenopus 28S rRNA and yeast 26S rRNA.

Animals↗
PubMed1978

Serum lactate dehydrogenase in detecting liver damage associated with pre-eclampsia.

Assessment of liver damage by estimation of serum enzymes including lactate dehydrogenase (LDH) and its hepatic fraction, LDH5, was made in 60 normal pregnant women, 20 patients with pre-eclampsia and 10 with eclampsia. Definite evidence of damage was found in patients with pre-eclampsia and eclampsia and was related to the severity of the disease.

Alanine Transaminase↗