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Biomedical subjects

R K Sumarah

Publications and source records attributed to R K Sumarah.

6 recordsLinked to original sources
PubMed1984

Isolation of Legionella pneumophila from the blood of a patient with Legionnaires' disease.

Legionella pneumophila is rarely isolated from blood cultures. Presently most cases of Legionnaires' disease are diagnosed retrospectively from the results of indirect fluorescent antibody tests, which possess inherent disadvantages. An 81-year-old woman with a history of diabetes mellitus presented symptoms of Legionnaires' disease. Five hours before her death 1.5 mL of blood was withdrawn from a scalp vein and seeded to a culture medium. Following incubation for 3 days L. pneumophila serotype 1 was isolated.

Aged↗
PubMed1984

A synthetic based medium for the isolation of Listeria monocytogenes.

We have compared two media (Tryptone Soya Nalidixic Acid Agar and Tryptone Soya Gum Base Nalidixic Acid Medium) for the isolation of L. monocytogenes from pathological and environmental specimens. These media were used in conjunction with Henry's Oblique Light System. We found that the gum based medium gave the better results. The medium is uniformly transparent, partially suppressive to unwanted organisms and allows colonies of Listeria to be readily identified.

Culture Media↗
PubMed1978

Assessment of expectorated sputum for bacteriological analysis based on polymorphs and squamous epithelial cells: six-month study.

Samples of sputum were examined microscopically to determine their suitability for routine culture. When the number of squamous epithelial cells per field was less than 10, the number of bacterial species generally fell within the range of one to four. Squamous epithelial cells were not always a true indication because some unmarked transtracheal specimens showing more than 10 squamous epithelial cells also gave a range of isolation falling between one and four. When the presence of 25 or more polymorphs was used as the parameter, the number of bacterial isolates generally fell within the range of one to three, but this resulted in positive overbiasing with consequent rejection of valid specimens. Later it was found that when a differential system using both polymorphonuclear cells and squamous epithelial cells was applied, a significant number of specimens could be salvaged which would otherwise have been discarded.

Epithelial Cells↗
PubMed1975

Comparison of four culture media for the isolation of Mycobacterium tuberculosis: a 2-year study.

Four media, Lowenstein-Jensen, Middlebrook, Petragnani, and ribonucleic acid, were tested for comparative ability to detect Mycobacterium tuberculosis. Specimens used included sputum, urine, tissue, and gastric washings. Three types of comparison were used: (i) comparison derived from randomized specimens; (ii) comparison of cultures from newly diagnosed cases that had received no prior therapy; and (iii) comparison of cultures from specimens whose initial direct smears were negative. Overall, ribonucleic acid medium performed best, but the differences among the four media were small.

Cerebrospinal Fluid↗