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R K Tompkins

Publications and source records attributed to R K Tompkins.

At least 127 records · Page 7Linked to original sources
PubMed1970

Peptide chain termination. VII. The ribosomal and release factor requirements for peptide release.

Release factors participate in release of fMet from fMet-tRNA . AUG . ribosome intermediates upon binding to ribosomes. This release requires R factor and occurs in the absence of terminator codon in reactions containing 20 per cent ethanol. Release occurs only when both 30S and 50S ribosomal subunits are present and when fMet-tRNA is located in the ribosomal P site. Release factor-dependent deacylation of fMet-tRNA is inhibited by sparsomycin, amicetin, lincocin, and chloramphenicol, antibiotics which have little effect on binding of R factor to ribosomes. The possible role of peptidyl transferase in the release reaction is discussed.

Amino Acid Sequence↗
PubMed1970

Sequential translation of trinucleotide codons for peptide bond formation, translocation, and termination.

The addition of GUA, valyl-tRNA, T factor, GTP, and ethanol to reactions containing fMet-tRNA.AUG.ribosome intermediate results in the synthesis of fMet-valyl-tRNA which can be translocated if G factor is also present. The translocated peptidyl-tRNA can interact with puromycin to form fMet-Val-puromycin. Release of fMet-valine can also take place after translocation if a terminator trinucleotide and its specific release factor are present.

Carbon Isotopes↗