Biomedical subjects
R Kajioka
Publications and source records attributed to R Kajioka.
An assessment of urease-based enzyme-linked immunosorbent assay.
The use of urease in enzyme-linked immunosorbent assays (ELISAs) offers the advantages of convenience and safety. However, urease-based ELISA, performed in standard microtitre format, could result in false positive reactions upon prolonged incubation. False positive reactions appeared when wells containing substrate solution absorbed ammonia liberated from a reactive well nearby. Thus, the intensity of the false reaction was proportional to that of the urease reaction. The transfer of ammonia was demonstrated by pyrolysis-mass spectrometry. When urease conjugates were compared with peroxidase conjugates in the detection of IgG and IgM, there was no evidence that one enzyme was superior to the other in terms of increasing the sensitivity or the speed of ELISA.
Comparison of Gen-Probe commercial kit and culture technique for the diagnosis of Mycoplasma pneumoniae infection.
The Gen-Probe rapid diagnostic system was compared with a culture method for the detection of Mycoplasma pneumoniae in clinical specimens. Of 116 clinical specimens, 103 (88.8%) yielded identical results. The relative sensitivity and specificity of the probe were both 89%. Rapid turnaround time and its sensitivity and specificity indicate that the probe test is a practical method for the rapid diagnosis of M. pneumoniae infections.
The pleiotropic effect of spontaneous single-step variant production in Mycobacterium intracellulare.
A strain of M. intracellulare, AT 13786, derived from human sputum, gave rise to transparent and opaque colony forms which were cloned and investigated. From the results the conclusion was drawn that the opaque form was a mutant of the transparent type and possessed alterations in the cell envelope which were responsible for enhanced permeability. The opaque form was more susceptible to a number of antibiotics; it grew faster in standard medium, and was not dependent on tween for dextrose utilization. Tween enhanced the antibiotic susceptibility of the opaque form more than that of the transparent. The avirulent opaque colony form of the pathogenic strain serotype Boone also revealed a loss of tween dependence for dextrose utilization. The significance of the overall change has been discussed with respect to the question of virulence.
Permeability barrier to rifampin in mycobacteria.
A strain of Mycobacterium intracellulare that exhibited natural resistance to rifampin was isolated. The ribonucleic acid polymerase was found to be susceptible to rifampin. Studies with Tween 80 suggested that a permeability barrier against rifampin was present in the intact organism. A type strain of Mycobacterium smegmatis, ATCC 607, considered resistant to rifampin also demonstrated a permeability barrier. The possibility that rifampin resistance in naturally occurring strains of mycobacteria and variable levels of resistance in strains of certain species of this organism are due to permeability barriers is discussed.
Some studies on the status of polyoma-specified RNA after transcription.
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