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R Kano

Publications and source records attributed to R Kano.

At least 19 recordsLinked to original sources
PubMed1998

Molecular analysis of chitin synthase 1 (CHS1) gene sequences of Trichophyton mentagrophytes complex and T. rubrum.

Nucleotide sequences of chitin synthase 1 (CHS1) gene of dermatophytes, Arthroderma benhamiae, A. simii, A. vanbreuseghemii, Trichophyton mentagrophytes var. interdigitale (T. interdigitale), and T. rubrum were analyzed for their phylogenetic relationship. About 620-bp genomic DNA fragments of the CHS1 gene were amplified from these dermatophytes by polymerase chain reaction (PCR) and sequenced. The CHS1 nucleotide sequences of these five dermatophytes showed more than 90% similarity between the species. The phylogenetic analysis of their sequences revealed that A. benhamiae, A. simii, A. vanbreuseghemii, and T. rubrum were genetically distinct from one another, but T. interdigitale was genetically very close to A. vanbreuseghemii. On the other hand, a specific restriction endonuclease site of HinfI was present in the CHS1 gene fragment of T. rubrum but not in those of A. benhamiae, A. simii, A. vanbreuseghemii and T. interdigitale. The molecular analysis of CHS1 genes will provide useful information for the identification of these Trichophyton species and the understanding of their evolution.

Animals↗
PubMed1998

The first isolation of Arthroderma benhamiae in Japan.

A clinical isolate of Trichophyton mentagrophytes from rabbit was examined by polymerase chain reaction (PCR) analysis and a mating experiment. The species-specific primers designed from the nucleotide sequences of the chitin synthase 1 (CHS1) gene in the teleomorph of Arthroderma benhamiae amplified a fragment from genomic DNA samples of A. benhamiae and the clinical isolate but not from those of A. simii and A. vanbreuseghemii. On the other hand, the species-specific primers of A. simii and A. vanbreuseghemii did not amplify any fragment from the genomic DNA of the clinical isolates. When the isolate was respectively crossed with (+) or (-) tester strains of A. benhamiae, A. simii and A. vanbreuseghemii, ascospores were produced in the crossing with the A. benhamiae (+) strain. Therefore, the isolate was identified to be A. benhamiae (-), confirming the result of molecular analysis. This is the first report on the isolation of A. benhamiae in Japan.

Animals↗
PubMed1997

Phylogenetic analysis of 8 dermatophyte species using chitin synthase 1 gene sequences.

Nucleotide sequences of chitin synthase 1 (CHS1) gene of eight species of dermatophytes, Arthroderma benhamiae, A. fulvum, A. grubyi, A. gypseum, A. incurvatum, A. otae, A. simii and A. vanbreuseghemii were obtained and analysed for their phylogenetic relationship. A 600-bp genomic DNA fragment of the CHS1 gene was amplified from these dermatophytes by polymerase chain reaction (PCR) and sequenced. The CHS1 nucleotide sequences of these eight dermatophyte species showed more than 85% similarity between the species. The phylogenetic analysis of their sequences revealed three clusters, the first cluster consisting of A. benhamiae, A. simii and A. vanbreuseghemii, the second cluster consisting of A. fulvum, A. gypseum and A. incurvatum, and the third cluster consisting of A. grubyi and A. otae. The phylogenetic analysis of CHS1 gene in this study will provide useful information for classification and understanding the evolution of these dermatophyte species.

Arthrodermataceae↗
PubMed1997

Differentiation of Arthroderma spp. by random amplification of polymorphic DNA (RAPD) and Southern hybridization.

To develop the molecular differentiation analysis of dermatophytes, we carried out RAPD and Southern hybridization analyses using genomic DNAs of six Arthroderma species, including A. fulvum, A. grubyi, A. gypseum, A. incurvatum, A. otae and A. racemosum. The RAPD analysis gave different band patterns specific to each of the six Arthroderma fungi. However, minor differences in the banding patterns were observed between the strains of plus (+) and minus (-) mating types of A. gypseum, A. fulvum and A. incurvatum. Southern blot analysis using a probe (1S) obtained from A. grubyi DNA gave specific bands only in the DNA samples of A. grubyi and A. incurvatum. On the other hand, Southern blot analysis using a probe (C3) obtained from A. otae DNA gave specific bands in all six Arthroderma species examined, and the size of the bands were specific to each species. These findings indicate that RAPD and Southern hybridization analyses are useful in the differentiation of these Arthroderma species.

Amino Acid Sequence↗
PubMed1997

A case of feline cryptococcosis treated with itraconazole.

We successfully treated a feline case of cryptococcosis with itraconazole (ITZ) at a lower dosage. The patient was a 2-year-old castrated male Abyssinian cat weighing 4.1 kg and with two masses on the head. Clinical signs were sneezing and nasal discharge. The plasma cryptococcal antigen titer measured by the latex agglutination test was proved to be high (512). The biopsy specimen from the masses disclosed yeast cells which were cultured and identified to be Cryptococcus neoformans. The cat was treated with ITZ 5 mg kg-1 given orally once a day with food. After 4 weeks, treatment of ITZ discontinued, because the cat was clinically normal and the antigen titer was low (128). However, about 7 months later, a subcutaneous nodule was detected on the same area. The nasal discharge appeared again, and the cryptococcal antigen titer was 256. ITZ treatment was continued again at the same dosage for 3 months until the antigen titer was negative (< 8). Four months after discontinuation of ITZ, the cat did not relapse and the antigen titer was in the negative range. No side-effects of ITZ were detected by physical and laboratory examination.

Animals↗
PubMed1996

Canine parvovirus binds to multiple cellular membrane proteins from both permissive and nonpermissive cell lines.

For identification of canine parvovirus (CPV) binding protein, the SDS-solubilized cell membrane fraction from a permissive cell line. CRPK, was subjected to the virus overlay protein blot assay (VOPBA). Competitive inhibition experiments showed the presence of multiple CPV-binding proteins with molecular masses of 36, 35, 33, 31, 29, 27, 25, and 23 kDa. CPV-binding proteins of same molecular masses were also detected in membrane fractions from nonpermissive, as well as other permissive, cell lines. We confirm that the mechanism of nonpermissiveness to CPV is not operative at the cellular attachment level.

Animals↗
PubMed1987

Application of a screening test for depression in the Health Counselling Center.

This paper outlines the process of development and the clinical usefulness of a screening test for depression. First we developed a draft questionnaire consisting of 13 items, each of which had three answers--(1) often, (2) sometimes, (3) none--, with reference to certain kinds of rating scales. After each answer was quantified by the sigma method, each item was statistically analyzed among 75 depressive patients and 250 almost healthy subjects visiting the AMHTS (Automated Multiphasic Health Testing and Services) center. Then 12 items effective for discriminating between the two groups were computed by discriminant analysis to produce depression scores which varied from 0 to almost 100 in proportion to the severity of depression. This questionnaire was applied to all consultees visiting the AMHTS, and revealed that almost 1% of them showed higher scores than the threshold score. Psychiatric interviews with the subjects suspected of depression indicated that all of them were in the transient maladapted state although only ten out of 16 were referred to a psychiatric outpatient clinic for further examination and treatment. The validity of this questionnaire was also confirmed by the fact that 86% of 50 additional depressive patients showed higher scores than the threshold score.

Algorithms↗
PubMed1987

A case of intestinal myiasis in Japan.

A case of intestinal myiasis caused by larvae of Sarcophaga peregrina (Japanese name: senchi-nikubae) is reported. The patient passed stools containing live fly larvae for 2 days, but was otherwise asymptomatic. The patient seems to have been infested accidentally as a result of having eaten "yakitori" in which the fly had deposited its larvae. This paper is the first report of intestinal myiasis caused by the 3rd instar of this species.

Adult↗
PubMed1984

Phylogeny and geographical distribution of the genus Boettcherisca Rohdendorf (Diptera: Sarcophagidae).

The phylogeny of the genus Boettcherisca Rohdendorf was investigated by numerical techniques. The phenetic and phyletic dendrograms were obtained by mean character difference (MCD) and Russell and Rao's coefficient (SRR) proximity analyses and unweighted pair-group (UPGA) cluster analysis, and then related to the classification of the species group and the geographical distribution of the group. Two monophyletic and one paraphyletic groups were recognized: peregrina-group, septentrionalis-group and karnyi-group, respectively. A biological evolution was postulated commencing with a hypothetical ancestor having occurred in Sundaland and involving two lines of specialization, the peregrina-group found in and around Sundaland and the septentrionalis-group in Far East, Walacea and Papualand. On the basis of the cladogram and the present geographical distribution, a Sundaland origin was postulated for the genus.

Animals↗
PubMed1984

A case report of intestinal myiasis.

A case of mixed infection of Salmonella (type C2) and Parasarcophaga crassipalpis (an intestinal myiasis) in a two-year-old girl is reported. A girl after dining in a restaurant developed typical Salmonella food-poisoning symptoms. Stool culture showed both Salmonella and fly larvae. This paper is to report a first case of this fly larvae infection and to review the recent trends of food-poisoning in Japan.

Child, Preschool↗
PubMed1984

Susceptibility of the Danish housefly, Musca domestica Linné to pyrethroid.

The insecticidal activity of a new pyrethroid, fenvalerate, and the effects of its synergists were evaluated using te 49-s and 381-s strains from Denmark which are both resistant to pyrethroid. It was found that the effect of fenvalerate was poor on the pyrethroid-resistant strains and little was expected from the addition of its synergists. Rapid development of resistance was observed in the 49-s strain during the selection with permethrin.

Animals↗
PubMed1982

The role of the psychiatrist in the general hospital. I. Investigation and analysis pertaining the status of the psychiatrist in the general hospital.

Data for this study consisted of answers to a series of questions on the image of clinical psychiatry addressed to all the non-ppsychiatric clinicians in the Tokai University Hospital, and all the referral sheets for psychiatric consultation from non-psychiatric departments of the hospital for about 2 years since the beginning of the hospital's operation. The reaction was analysed as follows: (1) psychiatric services were requested for the reasons of "no organic abnormality" or for "differential diagnosis" in 59.4% of total referrals, (2) as many as 37.9% of non-psychiatric clinicians made no attempt to refer the patient to psychiatrists in spite of their recognition that these patients apparently needed psychiatric treatment, (3) trouble in doctor-patient relationship, if it was the real reason for asking for sychiatric services, was frequently not mentioned in the referral sheet, and (4) the reason for psychiatric consultation was rarely told clearly to the patient. On the basis of the above-mentioned findings, the role of the psychiatrist in the general hospital setting was reexamined.

Attitude to Health↗
PubMed1982

The role of the psychiatrist in the general hospital--II. Psychiatric patients with somatic complaints.

In part I, one of the important roles of the psychiatrist in the general hospital was pointed out with respect to the management and treatment of psychiatric patients with somatic complaints (PPSC) referred from other nonpsychiatric departments of the hospital. Part II deals with the way in which a PPSC is brought to the psychiatrist, and the basic principles concerning the initial interview which is crucial to the management and treatment of such patients. The principles for the initial interview are to clarify: (1) the processes through which the patient came to the psychiatrist, (2) the patient's motivation for receiving psychiatric help, (3) the nature of his relationship to the physician, and (4) related anxiety, etc. Referral of the patient to psychiatric treatment should not be done in haste, but advice to the physician must be given on maintaining or improving his relationship with the patient. Advice on how patients should be introduced to psychiatric treatment is included here.

Adult↗
PubMed1979

Levels of resistance to six synthetic insecticides in the Borneo housefly.

The resistant levels of the 4 strains of the housefly collected in Borneo to six insecticides, namely DDT, Chrysron, DDVP, Baytex, Sumithion and Diazinon, were examined. It was found that most housefly population in Borneo showed a higher susceptibility than that of the Takatsuki strain of Japan, especially to DDT.

Animals↗
PubMed1979

A preliminary key to the common calliphorid flies of peninsular Malaysia (Insecta: Diptera).

A key to the common Calliphorid flies of Peninsular Malaysia is presented. Illustrations of the genitalia of some rare species, list of new localities, altitudes and other ecological data are also presented. The following species were recorded for the first time from Peninsular Malaysia; Catapicephala sinica, C. kurahashii, Taninanina javanica, Hemipyrellia tagaliana, Lucilia sinensis, Blaesoxipha kasterni, Boettcherisca javanica, Parasarcophaga misera, P. orchidea, P. albicephs, Sarcosolomonia crinita, Thyrsocnema bornensis, Sarcorohdendorfia antilope and Lioproctia pattoni.

Animals↗
PubMed1979

Studies on medically important flies in Thailand. VI. Reports on 48 species of sarcophagid flies, including the taxonomic keys (Diptera: Sarcophagidae).

Flies from different geographical parts of Thailand were collected to study their medical importance as well as to make a zoogeographical study. After examination, 48 species of 19 genera, Kanomyia, Hosarcophaga, Blaesoxipha, Bercaea, Boettcherisca, Fengia, Lioproctia, Parasarcophaga, Phallosphaera, Rosellea, Sarcorohdendorfia, Sarcosolomonia, Sinonipponia, Pierretia, Thyrsocnema, Phytosarcophaga, Harpagophalla, Seniorwhitea, and Leucomyia belonging to Sarcophagidae were identified and those are reported in this paper. This study is based on the specimens available in the authors' collection.

Animals↗