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Biomedical subjects

R Kester

Publications and source records attributed to R Kester.

6 recordsLinked to original sources
PubMed1990

Extraction of ribosomal RNA from soil for detection of Frankia with oligonucleotide probes.

Sequences of 16S rRNA of the nitrogen-fixing Frankia strain Ag45/Mut15 and the ineffective Frankia strain AgB1.9 were used to design a genus-specific oligonucleotide probe. Hybridization experiments of this Frankia probe and a second probe, specific for Nif+-Frankia strains only, were used to detect Frankia specific target sequences in RNA isolations from soil. A method is described for direct isolation of RNA from a loamy soil and a peat. Yields of about 10 ng RNA/g wet soil are obtained without detectable contamination with humic acids. Isolation of RNA after initial extraction of bacteria from soil resulted in significantly lower RNA yields, compared to the direct isolation procedure. Hybridization with both probes against rRNA isolations from Frankia-containing soil could detect target sequences within RNA isolations from 1 g wet soil with an estimated detection limit of 10(4) cells.

Actinomycetales↗
PubMed1990

A comparison of teicoplanin vs. cephradine and metronidazole in surgical prophylaxis: an interim analysis.

The preliminary results of a trial to examine and compare the safety, tolerability and efficacy of a single dose of teicoplanin with three doses of cephradine combined with metronidazole are presented in a series of 113 patients undergoing elective vascular surgery. There were no obvious differences in the infection rates and sepsis indicators in either group. Neither drug regimen produced any evidence of renal or hepatic damage, though the levels of three hepatocellular enzymes, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and gamma-glutamyl-transferase (GGT), were seen to be transiently elevated, peaking 7 days post-operatively. The trial continues.

Administration, Rectal↗
PubMed1982

Nonstress test and maternal serum glucose determinations.

Serum glucose levels were measured in 50 nondiabetic pregnant women between 33 and 43 weeks' gestation following a nonstress test (NST) to determine the relationship between maternal glucose levels and the results of the NST. Two comparable groups of 25 patients each were formed based on the NST results, reactive and nonreactive. Overall, maternal glucose level for the study population was 71.3 +/- 20.1 mg/dl. Although the difference was not statistically different, the nonreactive NST group did demonstrate slightly higher glucose levels (71.6 +/- 17.3 mg/dl) than did the reactive group (70.9 +/- 17.3 mg/dl). Furthermore, patients studied for suspected intrauterine growth retardation or who had a history of a prior stillbirth and significantly lower (P less than .025) glucose levels than those tested for other indications. These results suggest that there is no correlation between the NST results and maternal serum glucose concentrations in a nondiabetic population. The significantly lower maternal glucose levels observed in intrauterine growth retardation and prior stillbirth groups warrant further investigation.

Adult↗