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R Kinsky

Publications and source records attributed to R Kinsky.

At least 37 records · Page 2Linked to original sources
PubMed1975

[Immunocytological characterization of cells recognizing antigen and producing antibody during immunization to oxazolone and E. coli lipopolysaccharide].

A preferential cell mediated response is obtained after stimulation with oxazolone () and a humoral response is seen after E. coli lipopolysaccharide (LPS) immunization (). Thus a cytological study of selectively isolated antigen recognizing cells which form rosettes and antibody producing cells which form hemolytic plages has been performed during oxazolone and lipopolysaccharide stimulations. This investigation shows quantitative and qualitative differences of these immunocyte subpopulations in the two stimulations. First the antigen recognizing cells antibody producing cells ratio is fundamentally much higher at all the stages of the oxazolone stimulation than after lipopolysaccharide immunization. On the other hand among these immunocyte subpopulations, T and B cells are identified by electron microscopy in the oxazolone immune reaction, while only B cells are observed after LPS immunization.

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PubMedDate not supplied

The in vivo antibody response to hen egg white lysozyme in H-2b-compatible responder and non-responder mice: is it regulated by its N-terminal peptide at the level of antigen-presenting cells?

We studied the in vivo antibody responses of three H-2b strains, BALB/b, C57BL/6 and BALB/B x C57BL/6 F1 to various lysozymes, REL and HEL, after priming with HEL, REL or the HEL N-terminal peptide. It was confirmed that C57BL/6 is a non-responder strain to HEL and that BALB/b is responder strain. The C57BL/6 non-responder trait was associated with HEL or peptide induction of suppressor cells, as shown by adoptive transfer experiments. We further demonstrated that the suppressor/non-responder trait is dominant in BALB/b x C57BL/6 F1 hybrids and that appropriately pulsed macrophages of BALB/b mice can bypass such suppression in these F1 mice. Possible mechanisms are discussed.

Amino Acid Sequence↗
PubMedDate not supplied

Electron microscopic study of thymus and bone marrow derived mouse lymphocytes: morphological differences and evolution of surface markers before and after in vitro mitogenic stimulation.

T and B lymphocytes from spleen, lymph nodes, thymus and bone marrow of unstimulated CBA mice have distinct ultrastructural features: respectively the Th type (dense dark appearance, smooth margin, high nucleocytoplasmic ratio, rare cytoplasmic organelles) and the Bm type (low electronic density, villous margin, low nucleocytoplasmic ratio, numerous cytoplasmic organelles). Correlations between Th or Bm morphology and presence of specific T or B surface markers (theta antigen or surface Ig) have been established. The Th/T and Bm/B equivalence do not however hold in all circumstances: first, there are morphologically intermediate types termed In (less than 13%) that may be theta- or Ig-positive; second, some Bm lymphocytes are theta-positive in CBA thoracic duct and some Th lymphocytes are Ig-positive in Nude mice spleen. Purified T-or B lymphocyte populations stimulated by selective mitogens (ConA or LPS respectively) undergo ultrastructural modifications before their surface markers (theta or Ig respectively) disappear. A time, some theta-positive T lymphocytes show a Bm-like morphology. The results suggest that the basis for the usual T-B ultrastructural differences in unstimulated mice resides in the normally different functional state of metabolic activity of these two types of cells: the cell metabolism would be higher in Bm (the usual form of unstimulated B lymphocytes) than in Th (the usual form of unstimulated T lymphocytes). This view may explain the paradoxical results observed in thoracic duct and Nude mice spleen as well as conflicting data reported by several authors.

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