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Biomedical subjects

R Klimas

Publications and source records attributed to R Klimas.

7 recordsLinked to original sources
PubMed1989

Polymorphonuclear neutrophils function in splenectomized patients.

Some essential functions of polymorphonuclear neutrophils (PMN) were evaluated in 30 patients splenectomized because of rupture of the spleen. These cells revealed normal random migration, adherence, unstimulated O2- and H2O2 production. Phagocytosis of viable staphylococci was higher than in controls, whereas chemotaxis, bactericidal capacity, aggregation and stimulated O2- and H2O2 production were significantly impaired. PMN from splenectomized patients manifested also the decreased intracellular myeloperoxidase activity. The percentage of cells with receptor for Fc IgG in peripheral blood was markedly decreased. Plasma of these patients induced increased adherence of autologous as well as control neutrophils. The possible mechanisms leading to the observed events are discussed.

Adolescent↗
PubMed1988

Polymorphonuclear neutrophils function in untreated patients with chronic myeloid leukemia.

Morphologically mature polymorphonuclear neutrophils (PMN) isolated from the peripheral blood of 30 patients were examined. These cells manifested the diminution of phagocytosis, random migration, chemotaxis, thermotaxis and bactericidal capacity. The surface charge of circulating PMN and the percent representation of cells with receptor for Fc IgG were significantly decreased. Stimulated as well as unstimulated oxygen consumption, production of O2- and H2O2 were significantly decreased, whereas the hexose monophosphate shunt activity was similar to that noticed in controls. The aggregation of leukemic PMN was practically normal, however their adherence was significantly higher. The possible mechanisms responsible for the observed PMN defects are discussed.

Adult↗
PubMed1987

Evidence of polymorphonuclear neutrophils (PMN) activation in patients with insulin-dependent diabetes mellitus.

Polymorphonuclear neutrophils' chemotaxis, surface charge, superoxide anions generation, NBT (nitro blue tetrazolium) reduction and intracellular lysozyme, and beta-glucuronidase content were estimated in patients with type I diabetes mellitus in a similar state of metabolic control. The chemotaxis of diabetic cells toward bacterial chemotactic factors was similar to controls, whereas migration toward complement-derived chemoattractants was significantly reduced. Polymorphonuclear neutrophils isolated from diabetic patients, when unstimulated, produced significantly greater amounts of superoxide anions and reduced NBT more efficiently. They also revealed reduced surface charge and lower intracellular content of lysozyme, whereas beta-glucuronidase content was similar to controls. The results obtained seem to indicate that neutrophils in patients with insulin-dependent diabetes manifest signs of being in the activated state. The possible mechanisms of such stimulation are discussed.

Adolescent↗
PubMed1986

A new micromethod for the evaluation of Fc receptor expression on polymorphonuclear neutrophils.

A simple method for the evaluation of rosetting polymorphonuclear neutrophils is described. It uses small (200 microliter) quantities of heparinized whole blood and is simpler than the micromethod proposed by Buescher and Gallin. The high concentration of neutrophils and paucity of lymphocytes considerably simplify the evaluation of specimens and make the technique particularly useful in granulopenic states. There was a close correlation between results obtained using this technique and those from a standard method requiring the isolation of polymorphonuclear neutrophils.

Bronchitis↗
PubMed1985

Prostatic calculi: a review.

Prostatic calculi are rare in children, infrequent below age 40, and common in males over 50. They may be solitary but usually occur in clusters and are associated with some other disease process (nodular hyperplasia, prostatic carcinoma, metabolic abnormalities). They are most often asymptomatic; however, symptoms that may be attributable to prostatic calculi include reduction of the urinary stream, prostatism, and intense lower back and leg pain. Treatment may be accomplished by transurethral resection, but prostatectomy is the best means to insure complete removal. Prostatic calculi may form by two related mechanisms with obstruction and stasis of prostatic fluid as central entities in both. These mechanisms are calcification of corpora amylacea and simple precipitation of prostatic secretion. They may arise spontaneously, initiating an inflammatory reaction that contributes to their growth, or they may arise as the consequence of another pathologic situation producing acinar obstruction. It appears that infection probably occurs secondary to stone formation.

Adult↗
PubMedDate not supplied

The influence of increasing glucose concentrations on selected functions of polymorphonuclear neutrophils.

The influence of increasing glucose concentrations on some essential functions in polymorphonuclear neutrophils (PMN) was evaluated in vitro in 20 healthy persons. PMN chemotaxis, adherence, phagocytosis and bactericidal capacity were estimated. Higher than physiologic glucose concentrations were found to diminish chemotaxis, phagocytosis and bactericidal capacity. The results obtained may indicate another possible mechanism that accounts for the increased susceptibility to infection observed in diabetic patients. However, PMN adherence rose parallel with increasing glucose concentrations reaching maximum values at 300 mg/dl (16.6 mmol/l). The changes of PMN adherence may suggest the participation of these cells in the development of microangiopathic injuries.

Adult↗