Letter: Immune complexes in kidneys of rats treated with antiserum to luteinising hormone.
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Abortion, Induced↗
Biomedical subjects
R Kofler
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Placental hormones: I. Immunofluorescence studies of the localization of chorionic gonadotrophin, placental lactogen and prolactin in human and rat placenta and in the endometrium of pregnant rats.
Chorionic gonadotrophin (CG), placental lactogen (PL) and prolactin (PRL) were localized in sections of human and rat placenta using the indirect immunofluorescence technique. Antisera were absorbed with homologous and unrelated antigens by affinity chromatography or complexed with their respective antigens. To exclude binding of IgG and immune complexes to Fc-receptor in chorionic tissue, thus leading to erroneous results, sections were preincubated with an excess of rat IgG. The results indicate that, in the human placenta, human CG (hCG) and human PL (hPL) can be identified in the syncytiotrophoblast. Using antibodies to hPRL fluorescence was found in giant cells within the cytotrophoblastic islands in the intervillous space and in cytotrophoblastic cells within placental septa. In the rat placenta rat PL (rPL) and rat PRL (rPRL) could be identified in the giant cells of the basal zone. Furthermore rPRL/rPL was localized in the endometrium and adjacent decidial cells of rat pregnancy. No staining of chorionic cells was detected using antibodies against hCG and bLH, which cross-reacted with rLH. These experiments provide further evidence that the rat placenta is unlikely to produce a CG/LH-like hormone.
Animals↗
Placental hormones: II. Immunofluorescence studies of the localization of prolactin/placental lactogen- and human chorionic gonadotrophin-receptors in human and rat placenta.
Human term placentae and rat placentae of gestation day 15 and 17 were examined for the localization of prolactin (PRL)/placental lactogen (PL) and human chorionic gonadotrophin (hCG) receptors by means of an immunofluorescence technique in order to determine possible target cells for PRL/PL and hCG and to obtain indirect evidence for the localization of steroid producing cells. In the human placenta, PRL/PL- and hCG-receptors were observed in the syncytiotrophoblast of the chorionic villi and in the giant cells of placental septa and cytotrophoblastic islands in the intervillous space. In the rat placenta, PRL/PL- and hCG-binding was localized mainly to the giant cells and vacuolated glycogen cells of the basal zone.
Animals↗