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R Konno

Publications and source records attributed to R Konno.

84 records · Page 5Linked to original sources
PubMed1984

Brain and kidney D-amino acid oxidases are coded by a single gene in the mouse.

Mutant mice (ddY/DAO-) lacking D-amino acid oxidase in the kidney also lacked this enzyme in the brain. Genetic cross experiments showed that the inheritance of the enzyme in the brain was the same as that in the kidney. The deficiency in the enzyme in the brain could not be separated from that in the kidney. The brain and kidney enzymes showed similar substrate specificities. These results suggest that brain and kidney D-amino acid oxidases are coded by the same gene in the mouse.

Animals↗
PubMed1983

Mouse mutant deficient in D-amino acid oxidase activity.

D-Amino acid oxidase activity in the kidney homogenates of mice of seven strains was measured to search for a mutant for this enzyme. There was a consistent sex difference in the enzyme activity in these strains: male mice showed higher levels of the enzyme activity than females. In contrast to other strains, some mice of the ddY strain did not possess enzyme activity. This trait was inheritable, and a mouse stock without enzyme activity (DAO-) was established. The allele (Dao-1c) carried by the DAO- mice was recessive and behaved as a single autosomal gene in inheritance. Heterozygous mice for this gene (Dao-1+/Dao-1c) showed nearly half the enzyme activity of the wild-type homozygotes (Dao-1+/Dao-1+), suggesting that Dao-1c is a null allele and that there is a gene dosage effect on the enzyme activity.

Alleles↗
PubMed1982

Intraspecies and interspecies variations in the substrate specificity of D-amino acid oxidase.

1. Substrate specificity of D-amino acid oxidase was examined in the kidney homogenates of the mouse and the six species of fishes. 2. The enzyme of the mouse did not show a significant intraspecies variation in the substrate specificity. The sex and age of the mouse did not affect the substrate specificity of the enzyme. 3. The degree of similarity in the substrate specificity of the enzymes was quantified as the variation index. The values of the variation index among the enzymes of the fishes paralleled their taxonomic relatedness.

Animals↗
PubMedDate not supplied

Adenoma malignum. Report of a case with cytologic and colposcopic findings and immunohistochemical staining with antimucin monoclonal antibody HIK-1083.

BACKGROUND: Adenoma malignum of the uterine cervix was first described by Gusserow. We report here a case with cytologic, histologic and colposcopic findings and immunohistochemistry for HIK-1083. CASE: A 42-year-old female was noted to have a probable adenoma malignum due to the detection of atypical cells classified as V. On colposcopy, comma-shaped, atypical vessels spread over the entire cervical area. Histologic findings were characteristic of tumor invasion beyond the layer of cervical glandular ducts. Immunohistochemical detection of CEA was negative, but HIK-1083, which recognizes gastric glandular mucous cells, was positive. CONCLUSION: For a definitive diagnosis of adenoma malignum of the cervix, immunohistochemical examination for an appropriate marker, such as HIK-1083, should be added to the routine gynecologic examination, cytologic and histopathologic examination, and colposcopy.

Adenocarcinoma, Mucinous↗
PubMedDate not supplied

Mass screening for cancer of the endometrium in Miyagi Prefecture, Japan.

OBJECTIVE: To survey the results of mass screening for cancer of the endometrium performed over a six-year period in Miyagi Prefecture, Japan. STUDY DESIGN: Materials were cytodiagnostic samples of the endometrium examined by the Miyagi Cancer Society. The samples were classified into two groups: The mass screening group, from whom samples were collected according to the Health and Medical Service Law for the Aged, and the outpatient group, consisting of samples from other patients. The rates of subjects judged to require close examination and the detection rate of cancer of the endometrium in the two groups were compared. RESULTS: In the mass screening group, the rate of subjects judged to require close examination and the detection rate of cancer of the endometrium were 2.3% and 0.11%, respectively, while they were 5.9% and 0.39%, respectively, in the outpatient group; the differences between the two groups were significant. CONCLUSION: To improve the detection rate in mass screening for endometrial cancer according to the Health and Medical Service Law for the Aged, it is necessary to establish a new criterion for selecting subjects.

Endometrial Neoplasms↗
PubMedDate not supplied

Mass screening for cancer of the uterine cervix in Miyagi Prefecture, Japan. Effects and problems.

OBJECTIVE: To evaluate the effects of and problems with mass screening and in Miyagi Prefecture, Japan. STUDY DESIGN: About 3,570,000 females who underwent mass screening for cervical cancer between 1962 and 1994. RESULTS: The screening rate in the female population aged 30 years or older was 9.5% in 1970, gradually increased and exceeded that rate in 1991 but showed only slight changes thereafter. The standardized death rate due to malignant neoplasm of the cervix fell from 12.1 per 100,000 in 1961 to 4.0 in 1994. CONCLUSION: There still remain various problems, such as lack of a further increase in the screening rate, the fixation of examinees and an increase in the incidence of cervical cancer in young females.

Adult↗
PubMedDate not supplied

Cytologic application of p53 overexpression using immunocytochemistry and confocal laser scanning microscopy.

OBJECTIVE: p53 Protein plays an important role in cellular growth control. This study investigated p53 protein expression in cell smears of endometrial carcinomas supplemented by confocal laser scanning microscopy (CLSM). STUDY DESIGN: Imprints from surgical specimens of 20 endometrial carcinomas were used. p53 Protein expression was investigated immunocytochemically using the monoclonal antibody pAb1801. Using CLSM, three-dimensional morphology was studied. RESULTS: Of the 20 cases of endometrial carcinoma, 8 stained positively for p53 protein. p53 Showed heterogeneous intranuclear localization, which appeared to be associated with chromatin structure. CONCLUSION: Immunocytochemical detection of p53 overexpression in cell samples is practical, and CLSM has vast potentials in studying the intranuclear arrangement of chromatin.

Animals↗
PubMedDate not supplied

Prognostic features of cervical dysplasia associated with specific types of HPV DNA and cytologic features characteristic of HPV infection in dysplasia.

OBJECTIVE: To study a possible etiologic relationship between the prognosis of uterine cervical dysplasia, association of type-specific human papillomavirus (HPV) DNA and cytologic features characteristic of HPV infection. STUDY DESIGN: Two hundred thirteen cases of uterine cervical dysplasia were selected in which follow-up survey for more than two years was possible. Frequency of the presence of HPV DNA in the DNA samples was determined by polymerase chain reaction. The cervical scrapings were also examined microscopically for the frequencies of cells with cytologic features characteristic of HPV infection. RESULTS: HPV was positive in 98 cases (46.0%). The high-risk type of HPV was detected at almost the same frequencies in both progressive and regressive states of dysplasia. Cytologic features were more evident in cells infected with the low-risk type of HPV. CONCLUSION: Involvement of an as-yet-unknown factor or factors coupled with infection with the high-risk type of HPV is implicated in the progression of uterine cervical dysplasia. Cytologic features characteristic of HPV infection may serve as a diagnostic marker for a favorable prognosis in dysplasia.

Carcinoma in Situ↗
PubMedDate not supplied

Immunohistochemistry of Caspase3/CPP32 in human stomach and its correlation with cell proliferation and apoptosis.

Caspase3/CPP32 is a member of the interleukin-1 beta-converting enzyme (ICE) or cell death effector (CED)-3 family, which is involved in the induction of apoptosis and has been considered to be correlated with apoptosis because of the most downstream enzyme in their apoptosis inducing pathway. We immunolocalized Caspase3/CPP32 in both normal and neoplastic human gastric mucosa. We then correlated the findings with cell proliferation studied by Ki67 immunostaining and apoptosis, which was tested for by DNA fragmentation in situ using TdT-mediated dUTP biotin nick end labeling (TUNEL) method in order to examine possible biological significance in cell turnover of normal and pathological human gastric tissues. Caspase3/CPP32 immunoreactivity was detected in both the cytoplasm and nuclei of glandular epithelial cells, predominantly in the Ki67 positive proliferative zone and TUNEL positive foveolar epithelium of normal non-neoplastic gastric mucosa (n = 10) and tumor cells of both adenoma (n = 17) and carcinoma (n = 33). We determined the labeling index (LI) of Ki67, Caspase3/CPP32 and TUNEL positive cells by evaluating the number of positive cells in the same areas of serial tissue sections using computer-assisted image analysis. Ki67 LI in adenocarcinoma (78.6 +/- 12.6%) was significantly [p < 0.0001] higher than that of adenoma (43.8 +/- 8.9%) and non-neoplastic gastric mucosa (24.2 +/- 9.0%). Caspase3/CPP32 LI in adenocarcinoma (17.1 +/- 10.3%) was significantly lower [p < 0.0001] than that of gastric adenoma (33.1 +/- 19.8%) and non-neoplastic gastric mucosa (42.4 +/- 15.8%). TUNEL LI in adenocarcinoma (1.9 +/- 2.1%) was significantly [p < 0.0001] lower than that of non-neoplastic gastric mucosa (6.0 +/- 3.5%), but not significantly different from that of adenoma (3.0 +/- 2.9%). These results indicate that gastric adenocarcinoma is associated with an inhibition of apoptosis and the augmentation of proliferative activity of tumor cells compared to non-neoplastic gastric mucosa. There was a tendency to a positive correlation between the Caspase3/CPP32 and TUNEL LI and an inverse correlation between the Caspase3/CPP32 and Ki67 LI, when evaluating all the specimens, although the correlation did not reach statistical significance. These results also suggest that Caspase3/CPP32 is involved in the development or regulation of apoptotic cell death in cell turnover of normal and neoplastic mucosa of the human stomach.

Adenocarcinoma↗