Calcium antagonists--future uses.
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Biomedical subjects
Publications and source records attributed to R Krebs.
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The 4 cases reported here are meant to illustrate the value of colour doppler-echocardiography in the diagnosis of ventricular septal rupture consecutive to an anterior myocardial infarction. With this method, the examination begins with colour doppler ultrasound superimposed on two-dimensional echocardiographic images and is completed by pulsed and continuous wave doppler velocimetry. In all 4 patients an abnormal colour doppler flow was visualized from the outset; it started in the left ventricule and crossed the ventricular septum to enter the right ventricule, thus confirming the diagnosis and locating the septal defect. This abnormal flow was easy to recognize. On the apical "4 cavity" projection it followed a semi-circular anti-clockwise course which occurred at each cardiac cycle. The flow was red at first in presystole at the apex of the left ventricule; it became blue in pre- and middle-systole while traversing the septum, then mosaic-like in middle- or end-systole around the septal defect on the right ventricular side, and finally blue again in pre-diastole within the right ventricle. The blue trans-septal flow persisted at least up to pre-diastole. The diagnosis of septal rupture was confirmed at surgery in 3 cases and at right heart catheterization in 1 case. Colour doppler-echocardiography is a simple, efficient and rapid method, superior to contrast echocardiography or pulsed doppler alone, for direct real-time visualization of the shunt. It makes the septal defect easy to locate, and it avoids invasive exploratory manoeuvres in cases where surgery is contra-indicated and/or when coronary angiography is not mandatory.
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The effects of various doses of human chorionic gonadetropine (HCG) to stimulate ovulation in 86 gilts in which puberty had been induced by administration of 500 IU of pregnant mare serum (PMS) and 250 IU of HCG were established by slaughter. Only 26.9 per cent of the group without HCG had completed ovulation 120 hours from puberty induction, but 93.5 per cent had done so in the group which had received additional 500 IU or HCG 78 hours after the PMS/HCG injection. Ovulation was completed by 71.4 per cent of those sows which had been stimulated, using 250 IU of HCG. More accurate timing of ovulation in animals of one and the same group can be helpful in better insemination timing.
A conclusion derived from the slaughter of 69 gilts was that no role was played by the time intervals tested between puberty induction, using 500 IU of PMS and 250 IU HCG, and subsequent action to stimulate ovulation. Very good follicle maturation and follicle formation as well as the usual uterus and ovary weights were observed, no matter whether 500 IU of HCG were injected to stimulate ovulation 54, 72 or 78 hours after puberty had been induced. Ovulation was very efficiently synchronised by 500 IU of HCG in all three groups in which the ovulation figures relative to follicle formation 120 hours from puberty induction were 92.6, 94.6 or 92.7 per cent.
Eighty gilts were slaughtered following different time intervals from biotechnical puberty induction, using 500 IU or PMS and 250 IU of HCG, and stimulation of ovulation, using 500 IU of HCG, 72 hours after puberty had been induced. The majority of those animals was found to have ovulated between 96 and 120 hours from puberty induction. Only 3.2 per cent of the follicles formed had undergone ovulation after 96 hours but as much as 94,0 per cent after 120. Not only had sexual maturity been reached by all animals, following the above treatment, but genuine superovulation was recordable from the first oestrus, with the average of formed follicles being 23.9. Stimulation of ovulation following puberty induction, therefore, is considered to be useful in providing favourable conditions for effective time-oriented insemination.
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Different frequencies and periods of insemination were checked with the view to optimising puberty induction to young sows, for which purpose 500 IU PMS/250 IU HCG were injected to 571 animals in seven experiments. Insemination was carried out by deadline, since follicle stimulation was exhibited by almost all animals following the above treatment, while only some of them displayed tolerance. Advantage was taken of the induced oestrus, since its ovulation rates were higher than those associated with subsequent heat cycles, and because a synchronised second oestrus was recordable only from some of those animals which exhibited follicle stimulation in response to puberty induction. The most favourable results in terms of pregnancy and number of embryoes, against the background of the great variability in ovulation onset, were obtained, if insemination was applied 72, 96, and 120 hours after gonadotrophin treatment. From among the variants with two inseminations, good results were obtained from those 96 and 120 hours after PMS/HCG treatment.
The PMS and HCG doses by which to induce puberty and successfully use the first induced oestrus were tested in three experimental slaughter series on 197 prepuberal young sows. An injection of 500 International Units of PMS and 250 IU of HCG was found to bear greatest promise. While higher dosages gave higher rates of ovulation and, following insemination, higher numbers of embryos, pregnancy rates as a whole dropped severely. Since a very high percentage of all animals involved exhibited follicle stimulation, following such treatment, while only half of them displayed tolerance, it is strongly suggested to use for all animals deadline-oriented rather than toleranceoriented insemination.
Four experimental series were applied to 342 prepuberal young sows to establish ovulation developments. Different periods of time were allowed to elapse between injection and slaughter. Injected were 400 IU PMS/200 IU HCG or 500 IU PMS/250 IU HCG. Onsets of ovulation were found to be highly differentiated and variable. Some of the animals had completed ovulation 72 hours after application of gonadotrophin, whereas in others had not even started 168 hours after such application. In the majority of all sows involved in the experiments ovulation occurred 96--144 hours from application of the gonadotrophis hormone. In other words, fertile semen should be present in the genital tract of sows in that period of time, if the concept of deadlineoriented insemination is followed.
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