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R L BOWMAN

Publications and source records attributed to R L BOWMAN.

18 recordsLinked to original sources

FLUORESCENCE POLARIZATION: MEASUREMENT WITH ULTRAVIOLET-POLARIZING FILTERS IN A SPECTROPHOTOFLUOROMETER.

Ultraviolet-polarizing filters with large apertures facilitate the use of spectrophotofluorometers for measuring fluorescence polarization. Such filters (i) allow the use of polarized exciting light even in the ultraviolet region, (ii) make it possible to correct for grating anomalies which influence the observed polarization, and (iii) because of good transmission properties allow the entrance of sufficient light so that small slits and dilute solutions can be used. Under optimum conditions, polarization can be measured with a precision of +/-0.002. Results obtained are in excellent agreement with values cited elsewhere and indicate that standard spectrophotofluorometers can easily be adapted for accurate measurement of polarization.

Coloring Agents↗

CONSTANT VOLUME, SELF-FILLING NANOLITER PIPETTE: CONSTRUCTION AND CALIBRATION.

Pipettes with volumes ranging from less than one nanoliter to 200 nanoliters can be constructed by means of a simple mechanical system and calibrated by radioisotope and fluorescence techniques. Biological fluids can be transferred with a repeatability of 1 percent by this self-filling pipette of constant volume.

Calibration↗

FREEZING-POINT DEPRESSION: NEW METHOD FOR MEASURING ULTRAMICRO QUANTITIES OF FLUIDS.

Accurate determination of the solute content of millimicroliter samples of biological fluids can conveniently be made with an instrument consisting of a thermoelectric cooler controlled by an electronic proportional servo system. Accuracies to within less than 2 percent have been achieved in determining the osmolarity of body fluids.

Body Fluids↗