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R L Erikson

Publications and source records attributed to R L Erikson.

135 records · Page 8Linked to original sources
PubMed1969

L-cell virion ribonucleic acid.

"L-cell virion," obtained from chronically infected 929 L-cell lines, was purified on 70% sucrose pads and its ribonucleic acid (RNA) was extracted and analyzed on sucrose gradients. Two RNA components were isolated from the virion, a rapidly sedimenting species (82S) and a more slowly sedimenting species (6S). When the fast-moving component was heated to 45 C, it yielded a homogeneous peak which now sedimented at approximately 72S. Upon heating to 70 C, the peak dissociates into a smaller homogeneous RNA species with a sedimentation coefficient of approximately 33S. Ribonuclease treatment and base composition analysis revealed that the fast-moving species is a single-stranded molecule. Base ratio analysis also revealed that the small (6S) RNA component is similar in its base composition to L-cell ribosomal RNA and distinctly different from the base composition of the large (72S) RNA component. Actinomycin D inhibits production of the L-cell virion by the L-cell line.

Centrifugation, Density Gradient↗
PubMed1967

Structure and function of bacteriophage R17 replicative intermediate ribonucleic acid. II. Properties of the parental labeled molecule.

Replicative intermediate ribonucleic acid (RNA), designated RI, which contained parental RNA labeled with (32)P was separated by filtration through agarose from the nucleic acids prepared from (32)P-labeled RNA phage-infected Escherichia coli. A larger amount of ribonuclease-sensitive parental label was found in the rapidly sedimenting forms of RI than in the slower sedimenting forms, indicating that parental RNA is displaced to form a single-stranded tail. This result indicates that some phage RNA is generated by asymmetric semiconservative replication of RI, but it does not mean that a portion of the RI duplexes cannot be conserved during generation of phage RNA. Parental RNA was also found in double-stranded RNA with no apparent tails which sedimented with an S value of 13. This RNA was soluble in 2 m NaCl, and its sedimentation rate was unaffected by ribonuclease; nevertheless, single-strand scissions were produced by ribonuclease and were detected after the duplex was converted to its component single strands.

Coliphages↗