Isolation and biological properties of endogenous baboon (Papio cynocephalus) type C viruses.
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Biomedical subjects
Publications and source records attributed to R L Heberling.
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A-type particles have been detected by electron microscopy in placentas from four strains of mice: AKR, Balb/c, C3H, and DBA/2N. Complete structures were observed within rough endoplasmic reticulum cisternae of the placental labyrinth cytotrophoblasts. A marked prevalence of such particles was observed in the DBA/2 N mice. Similar particles were also found in small cytotrophoblastic elements of the junctional zone (located between labyrinth and maternal decidua) of all mouse strains studied. The numbers of junctional zone particles in all four strains were roughly equivalent.
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Ultrastructural investigations have revealed the presence of apparent endogenously derived C-type particles in the placental villi of each of 13 baboons studied. Both budding and mature forms were observed in the syncytiotrophoblast of these animals at various stages of pregnancy.
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Herpesvirus recovery from normal and experimentally infected simian tissues prepared into 10 to 20% homogenates was compared with that obtained by explanting these same tissues. The explant procedure was by far the more sensitive of the two methods. As a result, 8 herpesvirus isolations were obtained by conventional virus isolation procedures in contrast to 74 isolates when explants were made from these same tissue sources. There is also evidence to suggest that the explant method is more sensitive than the use of tissue homogenates for recovery of other viruses as well.