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Biomedical subjects

R L Russell

Publications and source records attributed to R L Russell.

At least 19 recordsLinked to original sources
PubMed1995

Interactional structures of therapist and client participation in adult psychotherapy: P technique and chronography.

In previous studies, the utterances of 6 clients and their therapists in early, middle, and late sessions were rated on 34 and 39 speech categories, respectively. P-technique analyses revealed 4 client and 4 therapist factors. For the present study, therapist and client utterances were assigned factor scores. A 17 x 17 correlation matrix was constructed, consisting of correlations across 4 lagged utterances (therapist-client-therapist-client), with each utterance represented by 4 factor scores and a score for the third from which the utterance was sampled. Principal-components analysis of this matrix revealed 4 therapist-client interaction factors: Mutual Therapeutic Engagement, Therapeutic Negotiation, Undirected Client Reminiscence, and Sustained Therapist Work. Unsuccessful cases deviated most from successful cases on at least 1 factor. Comparisons of interaction chronographs of episodes drawn from a successful and unsuccessful case revealed meaningful differences. Discussion highlights the power of P technique to reveal structures of psychotherapeutic discourse.

Adult↗
PubMed1995

The paradox of the contented working woman in intercollegiate athletics administration.

We investigated the paradox of the contented working woman among middle and first-line intercollegiate athletics administrators. In this paradox, women report lower salaries than men but express comparable job satisfaction. The sample comprised 143 women and 371 men in 106 National Collegiate Athletic Association (NCAA) Division I-A institutions. Job satisfaction was measured with the Job Descriptive Index (Smith, Kendall & Hulin, 1969) and Job in General (Ironson, Smith, Brannick, Gibson & Paul, 1989) scales. Analyses revealed the existence of the paradox: Women were paid significantly less, but were equally satisfied with their jobs. Neither gender was satisfied with promotion opportunities or pay, but both were highly satisfied with their jobs in general--another apparent paradox. The disproportionate number of male respondents raised questions about gender equity in athletics administration.

Administrative Personnel↗
PubMed1995

Introduction to the special section on multivariate psychotherapy process research: structure and change in the talking cure.

In response to the search for novel investigative strategies to apply in the study of psychotherapy processes, several investigators have turned to the use of factor-analytic methods of investigation. Four studies are presented that use such methods to investigate the structure of, and change in, aspects of the client and therapist discourse and symptoms over the course of treatments. The corpora of process data, culled from investigations taking place on three different continents, represent some of the largest samples of in-therapy behavior that have been analyzed in microanalytic detail. Providing both methodological advances and important substantive findings, the studies included were conducted to stimulate further use and development of factor-analytic methods to identify and track key processes over the course of psychotherapeutic treatment.

Humans↗
PubMed1992

A reevaluation of estimates of child therapy effectiveness.

In a recent review of nonbehavioral child and adolescent psychotherapy, it was concluded that the magnitude of methodological flaws in this body of research precluded an evaluation of effectiveness. However, recent quantitative reviews of child and adolescent therapy have concluded that nonbehavioral therapy is relatively ineffective compared with behavioral therapy. A sample of nonbehavioral studies was reviewed to evaluate the adequacy of estimates of effectiveness. Three contributions to inaccurate effect estimation were considered: methodological quality, investigator allegiance effects, and treatment representativeness. It was concluded that all three factors contribute to inaccurate estimates of the effectiveness of child and adolescent psychotherapy. Recommendations for improving child therapy research are considered in light of these revealed problems.

Adolescent↗
PubMed1991

Correlation of cyclosporine and metabolite binding to cyclophilin and a 50 kDa binding protein with in vitro immunosuppression: a preliminary report.

Seven purified cyclosporine (CsA) metabolites were analyzed for binding to cyclophilin and to a 50 kDa protein purified from a JURKAT cell line. In addition, the potency of the seven metabolites, relative to CsA, was obtained using a primary mixed lymphocyte culture (MLC) suppression assay. CsA, M1, 17, and 21 were found to be immunosuppressive in the concentration range used (0-500 ng/mL). These results were then compared to protein binding. CsA and metabolite 17 (M17) bound to both proteins. Conversely, M1, 13, 21, and 26 bound only to cyclophilin, while M8 and M18 bound only to the 50 kDa protein.

Amino Acid Isomerases↗
PubMed1991

Language change in child psychotherapy: a meta-analytic review.

Eighteen child therapy outcome studies, containing 26 treatment/control comparisons on at least one language measure, were evaluated. Four questions were addressed: (a) Does child therapy positively impact children's language proficiencies, and are its effects comparable to those obtained on nonlanguage outcome measures? (b) Do outcome effects vary across individual versus group treatment? (c) Does the type of emphasis on verbal process in treatment affect the degree of gain in the children's language proficiencies at outcome? and (d) Do effect sizes on language measures differ depending on type of presenting problem? Results indicate that child therapy has a significant positive impact on children's language proficiencies and that individual is superior to group treatment. Several trends emerged suggesting relationships between the magnitude of language change and type of emphasis on verbal processes in therapy and the childs' presenting problems. Further research on language variables is recommended.

Child↗
PubMed1991

A preliminary study to evaluate an in vitro assay for determining patient whole blood immunosuppressive cyclosporine A and metabolite activity: comparison with cytosolic binding assays using cyclophilin or a 50-kilodalton binding protein, and the Abbott TDx cyclosporine A parent, and parent and metabolites assays.

Thirty-five allograft recipients undergoing cyclosporine A (CsA) therapy were randomly selected to evaluate a "novel" in vitro assay that determines CsA and metabolite immunosuppressive activity in whole blood. The assay uses a third party mixed lymphocyte culture (MLC) system to which patient whole blood extracts containing CsA and metabolites are added. The ability of the extracted CsA and metabolites to inhibit proliferation in this system is proportional to the immune suppressive activity in whole blood. Comparison of the MLC suppression assay against Abbott TDx parent, TDx parent and metabolites, and radioreceptor assays utilizing cyclophilin or a 50-kDa binding protein isolated from JURKAT cytosol gave the following correlation coefficients: r = 0.612, r = 0.672, r = 0.362, and r = 0.775, respectively.

Amino Acid Isomerases↗
PubMed1991

Immunoelectron microscopic examination of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus-infected Lymantria dispar cells: time course and localization of major polyhedron-associated proteins.

Immunoelectron microscopy was employed to examine the temporal expression and localization of two proteins involved in baculovirus polyhedron assembly (polyhedrin and p10) of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (OpMNPV) in infected Lymantria dispar cells. In addition, the association of p10 with the polyhedron envelope (PE) protein was studied. The major capsid protein (p39) was also examined to investigate the association of virion structural proteins with polyhedron formation. In infected cells, p39 did not show a concentrated association with any infected-cell structures other than nucleocapsids and appeared to be randomly distributed over the nucleocapsid surface. Likewise, polyhedrin showed no major concentrations outside of developing or mature polyhedra. The p10 antibody cross-reacted with a protein associated with condensed chromosomes in uninfected cells. In infected cells, p10 is a component of the body of fibrillar structures. The PE protein has been shown to accumulate around the periphery of fibrillar structures. Cells infected with a polyhedrin-minus virus expressing the beta-galactosidase gene under the control of the polyhedrin promoter were examined to determine whether the lack of polyhedra would influence the localization of major polyhedron-associated viral proteins. High concentrations of PE protein accumulating on the periphery of fibrillar structures appeared to be the major difference from wild-type virus-infected cells. The beta-galactosidase protein appeared to be distributed throughout the nucleus and cytoplasm, in contrast with the specific localization of the viral proteins.

Animals↗
PubMed1991

Effect of caffeine intake on psychotic in-patients.

Psychotic in-patients who consume caffeine may present complications in the course of their illness. Two cases are described which illustrate the clinical profile of such patients, whose caffeine-associated complications are often misdiagnosed.

Adult↗
PubMed1991

The posterior nervous system of the nematode Caenorhabditis elegans: serial reconstruction of identified neurons and complete pattern of synaptic interactions.

Serial-section electron microscopy has been used to reconstruct the cellular architecture of the posterior nervous system of the nematode Caenorhabditis elegans. Each of 40 neurons in the tail of the adult hermaphrodite can be reproducibly and unambiguously identified by a set of morphological features, including cell body position, fiber geometry and size, and staining properties. A complete list of synapses has been assembled for 2 isogenic animals, and these lists are compared with a third isogenic animal reconstructed by White et al. (1986). The set of neurons and their pattern of synaptic interactions is simple and reproducible. Most of the cells are involved in sensory transduction or in local signal processing to relay signals via a few interneurons to motoneurons and thence to body muscles. Because the tail neurons are well separated and fairly reproducible in position, the hermaphrodite tail lends itself to laser-ablation studies of sensory processing (cf. Chalfie et al., 1985). Most of the synapses in the tail are concentrated in the preanal ganglion. Among the approximately 150 synapses there, about 85% are dyadic chemical synapses. The dyadic synapses are involved in reproducible patterns that have several interesting features. Most neurons synapse onto a few preferred pairs of target cells, in patterns that suggest a combinatorial model of synapse specification that may be open to genetic analysis. Furthermore, most dyadic contacts A----B,C fit a pattern in which the 2 postsynaptic partners are involved elsewhere in unidirectional synapses B----C. Thus, the dyadic synapse may serve to diverge sensory signals into parallel pathways, which then reconverge. This divergence/reconvergence pattern eventually directs processed sensory signals to the ventral cord interneurons PVCL and PVCR. About 80-90% of the synapses fall into repeated classes of synapses. Many of the remaining synapses are widely scattered and irreproducible from one animal to the next. Some of these contacts may be developmental mistakes reflecting a degree of "noise" in synapse specification (Waddington, 1957).

Animals↗
PubMed1990

A baculovirus polyhedron envelope protein: immunogold localization in infected cells and mature polyhedra.

A polyclonal antiserum against a trpE fusion protein containing the complete open reading frame of the polyhedron envelope (PE) protein from the nuclear polyhedrosis virus of Orgyia pseudotsugata (OpMNPV) was used for immunogold staining and electron microscopic examination of polyhedra, isolated polyhedron envelopes, and infected insect cells at selected times postinfection. The antiserum specifically stained the peripheral envelope of mature polyhedra and also stained the envelope structure which remained after polyhedra were dissolved in dilute alkaline solutions. In OpMNPV-infected Lymantria dispar cells, the PE protein was detected by 48 hr postinfection (hr p.i.) but specific localization and staining of developing polyhedra were not evident. However, by 72 hr p.i. substantial and preferential staining of the periphery of developing polyhedra was evident even though a distinct polyhedron envelope was not yet observed. In addition, the periphery of fibrillar structures was stained by the PE antiserum. By 96 hr p.i., mature envelopes surrounded polyhedra and these polyhedron envelopes were stained with the PE antibody. The progression of PE protein staining during polyhedron morphogenesis indicates that the PE protein accumulates and becomes associated with developing polyhedra in the nucleus between 48 and 72 hr p.i. Very late in infection the mature polyhedron envelope forms on the polyhedron surface. The apparent affinity of the PE protein for the surface of maturing polyhedra suggests that it may be a major component of the polyhedron envelope or may form the matrix for the deposition of other components which contribute to the mature envelope. Immunogold staining and protease digestion experiments indicate that protein is an essential component of the polyhedron envelope.

Animals↗
PubMed1990

A capsid-associated protein of the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata: genetic location, sequence, transcriptional mapping, and immunocytochemical characterization.

Two lambda gt11 clones containing overlapping DNA inserts encoding portions of a structural protein gene from Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (OpMNPV) were identified by their immunoreactivity with polyclonal antisera produced against purified polyhedra-derived virus. Sequence analysis of a 3.6-kb region of the baculovirus genome (map units 69.1-71.6) from which the lambda gt11 inserts originated revealed an open reading frame of 1872 nt (624 amino acids) encoding a predicted protein of 70.6 kDa. Northern blot, primer extension, and 3' S1 analysis of this ORF indicated that an mRNA of approximately 2100 nt was transcribed from this gene. The mRNA appears to initiate from a late promoter/mRNA start site consensus sequence GTAAG and is expressed at late times postinfection. A gene fusion containing the C-terminal 368 amino acids of the gene was constructed using a bacterial trpE expression vector. Rabbit antiserum made against the purified fusion protein reacted with a protein of 87 kDa on Western blots of infected cell extracts at 24 hr p.i. and thereafter. The p87 protein was shown to be a component of both budded and polyhedra-derived virus and purified capsids. Immunofluorescence analysis indicated that p87 is expressed late in infection and concentrated in infected cell nuclei.

Amino Acid Sequence↗
PubMed1990

The p6.5 gene region of a nuclear polyhedrosis virus of Orgyia pseudotsugata: DNA sequence and transcriptional analysis of four late genes.

The gene encoding the basic DNA-binding protein (p6.5) of the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata (OpMNPV) was localized by Southern blot analysis using a cDNA probe containing the Autographa californica virus (AcMNPV) p6.9 gene. The OpMNPV p6.5 gene was mapped to the HindIII G fragment at map unit 67. Nucleotide sequence and transcriptional analysis of a 3.26 kb region encompassing this area revealed four open reading frames (ORFs 1 to 4) oriented in the same direction. ORF 1 demonstrated a seven codon overlap with ORF 2. Messenger RNAs initiated upstream of each of the four ORFs late in infection and were coterminal at a single site downstream of the fourth ORF. The conserved late gene promoter/mRNA start site sequence (ATAAG) was present upstream of all the ORFs, but did not appear to be the major site of mRNA initiation for the third ORF, as determined by primer extension analysis. The fourth ORF in this series encoded a predicted peptide of 51 amino acids (6.5K), which was 80% similar to the p6.9 basic DNA-binding protein of AcMNPV.

Amino Acid Sequence↗
PubMed1989

Quality assurance's role in reducing liability costs.

This article provides information on containing liability protection costs. The authors, experienced corporate and institutional liability protection specialists who provide risk management services to the medical and business community, describe the causes of the high cost of liability insurance and cost-saving alternatives to traditional insurance. Quality assurance's role in working with risk management to prevent losses and reduce costs in a self-insured program is covered. Insurance costs rise and fall in inevitable cycles, but the medical community can protect itself from these cycles. Quality assurance and risk management can work together to reduce costs and prevent losses. The principal conclusion is that through policies, procedures, and information exchange, quality assurance can expand its risk management role to help maintain high quality health care and financial stability. Within a self-insured liability program, direct savings to the institution will result.

Communication↗
PubMed1988

The acetylcholinesterase genes of C. elegans: identification of a third gene (ace-3) and mosaic mapping of a synthetic lethal phenotype.

In C. elegans, the newly identified ace-3 is the third gene affecting acetylcholinesterase (AChE) activity. ace-3 II specifically affects class C AChE and is unlinked to ace-1 X or ace-2 I, which affect the other two AChE classes (A and B, respectively). Strains homozygous for an ace-3 mutation have no apparent behavioral or developmental defect; ace-1 ace-3 and ace-2 ace-3 double mutants are also nearly wild type. In contrast, ace-1 ace-2 ace-3 triple mutant animals are paralyzed and developmentally arrested; their embryonic development is relatively unimpaired, but they are unable to grow beyond the hatching stage. Based on the analysis of genetic mosaics, we conclude that in the absence of ace-2 and ace-3 function, the expression of ace-1(+) in muscle cells, but not in neurons, is essential for postembryonic viability.

Acetylcholinesterase↗