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Biomedical subjects

R L Spooner

Publications and source records attributed to R L Spooner.

At least 91 records · Page 5Linked to original sources
PubMed1980

Bovine lymphocyte antigens (BoLA) of bovine lymphocytes and derived lymphoblastoid lines transformed by Theileria parva and Theileria annulata.

The BoLA phenotypes of lymphocytes from seven cattle have been compared with those of 19 lymphoblastoid cell lines derived from them by infection in vitro with either Theileria parva or T. annulata. Two long-established lines were also studied. In all cases except one, the BoLA phenotypes of the lymphoblastoid lines were identical with those of the original animal from whose lymphocytes they were derived. The one exception was a chimeric twin where a minor population appeared to have been transformed by T. parva. The antigens present on this line were present in the parents of the chimera, but not detectable in its own peripheral blood. The implications of these results as they relate to the use of these cell lins in immunizing cattle against East Coast fever and tropical theileriosis are discussed.

Animals↗
PubMed1980

Absorption analysis of boLA sera.

BoLA specificities w1 w2 w5 w8 w9 w10 and w11 have been re-examined by absorption. All of the clusters except w9 showed evidence of sub-group specificities, but only in some cases (w1, w5, w8 and w11) is it suggested that the definition should be revised. The results show a very high degree of reproducibility between sera from different laboratories with clusters of narrow and wide sera for most specificities. These behave in all the cases reported here as linear sub groups or inclusion groups.

Absorption↗
PubMed1979

Analysis of alloantisera against bovine lymphocytes. Joint report of the 1st International Bovine Lymphocyte Antigen (BoLA) workshop.

The results and agreements of the 1 international BoLA workshop, held in Edinburgh, Scotland in August 1978, are reported. Most of these concern the results from a comparison test of 249 alloantisera to bovine lymphocytes, the antisera being contributed by 9 laboratories. These sera were compared directly in Edinburgh on a panel of lymphocytes from 130 cattle of 21 breeds. In the microlymphocytotoxicity test used 75% of the sera reacted. Sixty eight of these sera were grouped into clusters according to their reaction patterns against the lymphocyte panel. Eleven of these clusters were clearly defined and were given workshop BoLA designations. In addition 22 sera were assigned to subgroups of the agreed clusters. There was no evidence that the method of production of the sera had any effect on their specificity. Although genetic data was not available, the phenotypes of the test panel of lymphocytes are consistent with the clusters detecting antigens controlled by multiple alleles at a single autosomal locus. It was agreed to name the genetic region where this putative locus is located BoLA (bovine lymphocyte antigen).

Animals↗
PubMed1979

The production and analysis of antilymphocyte sera following pregnancy and skin grafting of cattle.

This paper presents evidence to show that high titre antilymphocyte sera can be prepared more easily by skin grafting than from parous sera. It also shows that it is possible to analyse complex antilymphocyte sera by absorption with lymphocytes to produce working reagents. Following the 1 International BoLA Workshop the genotype of some of the animals used for skin grafting was ascertained and it was found that in each case where the resulting serum had been studied the main antiserum specificity that had been produced reacted with one of the BoLA specificities present in the skin donor.

Animals↗
PubMed1978

Further studies on bovine serum amylase--the effect of gel buffer pH.

This paper describes the effect of gel buffer pH on the resolution of bovine serum amylase (Amylase I) isozymes in starch gel and the consequences for the understanding of the genetics of this locus. The two main findings are: (1) the existence of a satellite isozyme E to isozyme C which at pH 7.3 has the same mobility as the B isozyme but which at pH 8.0 migrates slower than B, and (2) the finding of three alleles Aml A, Aml B and Aml C in British cattle populations previously reported as having only Aml B and Aml C.

Alleles↗
PubMed1977

Polymorphic variation in the amount of sialic acid attached to bovine transferrin.

In this paper family studies are presented which support the hypothesis of polymorphism in the process controlling sialic acid binding to bovine transferrin which modifies its phenotype as seen in starch gel electrophoresis. It has been shown that this polymorphism is controlled by locus Tfs with two alleles Tfs A and Tfs a. Tfs a/a animals have the abnormal phenotype with the two faster bands of the four bands of a normal transferrin allele being virtually absent. Tfs A/a and Tfs A/A are phenotypically normal. Limited evidence is presented which suggests that the Tf and Tfs loci are not linked.

Alleles↗
PubMed1977

Ox erythrocyte agglutinability. 4. The effect of neuraminidase treatment on the agglutinability of cells and ghosts.

1. The inherited differential agglutinability of cattle erythrocytes is shown to be similarly expressed on ghosts and intact cells. 2. Removal of virtually all sialic acid by prolonged neuraminidase treatment does not alter the agglutinability status of ghosts prepared from either high or low agglutinable cells. Hence the differing sialic acid content of the two cell types is not responsible for the differential agglutinability. 3. The significance of these findings with respect to other well defined agglutination systems and current theories of membrane structure is discussed.

Animals↗
PubMed1976

An IGG1 allotype in cattle.

This paper presents evidence for an IgG1 allotype detected by a sheep antibovine serum. The character which appears to be inherited in a simple Mendelian way has been named G1a1.

Animals↗
PubMed1975

Quantitative studies on bovine immunoglobulins.

The influence of serum immunoglobulins on the incidence of calf pneumonia, and the relationship between sub-clinical pneumonia, and calf serum Ig levels, is discussed. Monospecific antisera were used to measure levels of IgG1, IgG2, IgM and IgA in the sera of bull calves aged around 2 1/2 weeks. These calves were selected retrospectively according to their disease record up to six months of age. A clear association was found between low levels of IgG1, IgG2 and IgA in the "2 1/2-week" sample and subsequent susceptibility to pneumonia at around 2 1/2 months of age. Calves showing signs of pneumonia had low levels of IgG1 (45-5 per cent had less than 8 mg per ml compared with "now-pneumonic" calves which had relatively high levels (only 9.5 per cent had less than 8 mg per ml). In contrast, older calves bled at monthly intervals and found to have pneumonic lesions at slaughter had significantly evevated levels of both IgGa and IgG2.

Age Factors↗
PubMed1975

Quantitative studies on bovine immunoglobulins, normal plasma levels of IgG2, IgG1, IgM and IgA.

Using monospecific sheep antisera, the mean plasma immunoglobulin concentrations in 82 Ayrshire milking cows were found, by the single radial diffusion method, to be: IgG2 10-91 plus or minus 4-30, IgG1 9-76 plus or minus 2-59 and IgM 2-71 plus or minus 1-22 mg/ml (plus or minus SD). IgA levels in the same group of 82 cows were determined by a simple, sensitive coated tube radioimmunoassay and had a mean value of 0-32 plus or minus 0-16 mg/ml. The frequency distributions of IgG1, IgM and IgA were approximately log normal, whereas that of IgG2 was bimodal with two populations with means of approximately 7 and 14 mg/ml. An 18-fold difference was found between high and low IgG2 levels whereas only three- to four-fold differences were recorded for IgG1 levels in the same population. Eight-fold differences were found for IgM and IgA levels. The effect of age (2-9 years) on sampling could be disregarded for IgG1, IgG, IgM and IgA levels, but cows above 6 years of age had significantly higher IgG2 levels.

Age Factors↗